Expression of an Aspergillus niger Phytase (phyA) in Escherichia coli

Journal of Agricultural and Food Chemistry

Volumn 45, Issue 8, 1997, Pages 3337-3342

  Phillippy, Brian Q ^a   Mullaney, Edward J ^a  

^a SOUTHERN REGIONAL RESEARCH CENTER   (United States)

Author keywords

Aspergillus niger; Inositol phosphates; Phosphatase; Phytase; Phytic acid

Indexed keywords

ASPERGILLUS NIGER; ESCHERICHIA COLI;

EID: 0001122323     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf970276z     Document Type: Article

References (30)

1. Bradford, M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 72, 248-254.
2. Downs, D.; Xu, Y. Y.; Tang, J.; Wang, C. S. Proline-rich domain and glycosylation are not essential for the enzymic activity of bile salt-activated lipase. Kinetic studies of T-BAL, a truncated form of the enzyme expressed in Escherichia coli. Biochemistry 1994, 33, 7979-7985.
3. Ehrlich, K. C.; Montalbano, B. G.; Mullaney, E. J.; Dischinger, C. H., Jr.; Ullah, A. H. J. Identification and cloning of a second phytase gene (phyB) from Aspergillus niger (ficuum). Biochem. Biophys. Res. Commun. 1993, 795, 53-57.
4. Gabard, K. A; Jones, R. L. Localization of phytase and acid phosphatase isoenzymes in aleurone layers of barley. Physiol. Plant. 1986, 67, 182-192.
5. Greiner, R.; Konietzny, U.; Jany, Kl.-D. Purification and characterization of two phytases from Escherichia coli. Arch. Biochem. Biophys. 1993, 303, 107-113.
6. Han, Y. W.; Wilfred, A. G. Phytate hydrolysis in soybean and cottonseed meals by Aspergillus ficuum phytase. J. Agric. Food Chem. 1988, 36, 259-262.
7. Heinonen, J. K.; Lahti, R. J. A new and convenient colorimetric determination of inorganic orthophosphate and its application to the assay of inorganic pvrophosphatase. Anal. Biochem. 1981, 113, 313-317.
8. Irving, G. C. J.; Cosgrove, D. J. Inositol phosphate phosphatases of microbiological origin: the inositol pentaphosphate products of Aspergillus ficuum phytases. J. Bacteriol. 1972, 112, 434-438.
9. Kostrewa, D.; Grüninger-Leitch, F.; D'Arcy, A.; Broger, C.; Mitchell, D.; van Loon, A. P. G. M. Crystal structure of phytase from Aspergillus ficuum at 2.5 Å resolution. Nat. Struct. Biol. 1997, 4, 185-190.
10. Laemmli, U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227, 680-685.
11. LaVallie, E. R.; DiBlasio, E. A.; Kovacic, S.; Grant, K. L.; Schendel, P. F.; McCoy, J. M. A thioredoxin gene fusion expression system that circumvents inclusion body formation in the E. coli cytoplasm. Bio/Technology 1993, 11, 187-193.
12. Martins, L. O.; Santos, H. Accumulation of mannosylglycerate and di-myo-inositol-phosphate by Pyrococcus furiosus in response to salinity and temperature. Appl. Environ. Microbiol. 1995, 61, 3299-3303.
13. Maugenest, S.; Martinez, I.; Lescure, A.-M. Cloning and characterization of a cDNA encoding a maize seedling phytase. Biochem. J. 1997, 322, 511-517.
14. Melgaard, M.; Svendsen, I. Different effects of glycosylation on the thermostability of highly homologous bacterial (1,3-1,4)-β-glucanases secreted from yeast. Microbiology 1994, 140, 159-166.
15. Mitchell, D. B.; Vogel, K.; Weimann, B. J.; Pasamontes, L.; van Loon, A. P. G. M. The phytase subfamily of histidine acid phosphatases: isolation of genes for two novel phytases from the fungi Aspergillus terreus and Myceliophthora thermophila. Microbiology 1997, 143, 245-252.
16. Nair, V. C.; Duvnjak, Z. Reduction of phytic acid content in canola meal by Aspergillus ficuum in solid state fermentation process. Appl. Microbiol. Biotechnol. 1990, 34 183-188.
17. Pen, J.; Verwoerd, T. C.; van Paridon, P. A.; Beudeker, R. F.; van den Elzen, P. J. M.; Geerse, K.; van der Klis, J. D.; Versteegh, H. A. J.; van Ooyen, A. J. J.; Hoekema, A. Phytase-containing transgenic seeds as a novel feed additive for improved phosphorus utilization. Bio/Technology 1993, 11, 811-814.
18. Phillippy, B. Q. Identification by two-dimensional NMR of myoinositol tris- and tetrakis(phosphates) formed from phytic acid by wheat phytase. J. Agric. Food Chem. 1989, 37, 1261-1265.
19. Phillippy, B. Q.; Bland, J. M. Gradient ion chromatography of inositol phosphates. Anal. Biochem. 1988, 175, 162-166.
20. Piddington, C. S.; Houston, C. S.; Paloheimo, M.; Cantrell, M.; Miettinen-Oinonen, A.; Nevalainen, H.; Rambosek, J. The cloning and sequencing of the genes encoding phytase (phy) and pH 2.5-optimum acid phosphatase (aph) from Aspergillus niger var. awamori. Gene 1993, 133, 55-62.
21. Sakamoto, S.; Terada, I.; Lee, Y. -C.; Uehara, K.; Matsuzawa, H.; Iijima, M. Efficient production of Thermus protease aqualysin I in Escherichia coli: effects of cloned gene structure and two-stage culture. Appl. Microbiol. Biotechnol. 1996, 45, 94-101.
22. Sandberg, A.-S.; Hulthen, L. R.; Turk, M. Dietary Aspergillus niger phytase increases iron absorption in humans. J. Nutr. 1996, 126, 476-480.
23. Schein, C. H. Production of soluble recombinant proteins in bacteria. Bio/Technology 1989, 7, 1141-1149.
24. Simons, P. C. M.; Versteegh, H. A. J.; Jongbloed, A. W.; Kemme, P. A.; Slump, P.; Bos, K. D.; Wolters, M. G. E.; Beudeker, R. F.; Verschoor, G. J. Improvement of phosphorus availability by microbial phytase in broilers and pigs. Br. J. Nutr. 1990, 64, 525-540.
25. Ullah, A. H. J.; Cummins, B. J. Purification, N-terminal amino acid sequence and characterization of pH 2.5 optimum acid phosphatase (E.C. 3.1.3.2) from Aspergillus ficuum. Prep. Biochem. 1987, 17, 397-422.
26. Ullah, A. H. J.; Gibson, D. M. Extracellular phytase (E.C. 3.1.3.8) from Aspergillus ficuum NRRL 3135: purification and characterization. Prep. Biochem. 1987, 17, 63-91.
27. van Hartingsveldt, W.; van Zeijl, C. M. J.; Harteveld, G. M.; Gouka, R. J.; Suykerbuyk, M. E. G.; Luiten, R. G. M.; van Paridon, P. A.; Selten, G. C. M.; Veenstra, A. E.; van Gorcom, R. F. M.; van den Hondel, C. A. M. J. J. Cloning, characterization, and overexpression of the phytase-encoding gene (phyA) of Aspergillus niger. Gene 1993, 127, 87-94.
28. Verwoerd, T. C.; van Paridon, P. A.; van Ooyen, A. J. J.; van Lent, J. W. M.; Hoekema, A.; Pen, J. Stable accumulation of Aspergillus niger phytase in transgenic tobacco leaves. Plant. Physiol. 1995, 109, 1199-1205.
29. Yamada, K.; Minoda, Y.; Yamamoto, S. Phytase from Aspergillus terreus. Part 1. Production, purification, and some general properties of the enzyme. Agric. Biol. Chem. 1968, 32, 1275-1282.
30. Yoon, S. J.; Choi, Y. J.; Min, H. K.; Cho, K. K.; Kim, J. W.; Lee, S. C.; Jung, Y. H. Isolation and identification of phytase-producing bacterium, Enterobacter sp. 4, and enzymatic properties of phytase enzyme. Enzyme Microb. Technol. 1996, 18, 449-454.