Efficient production of Thermus protease aqualysin I in Escherichia coli: Effects of cloned gene structure and two-stage culture

Applied Microbiology and Biotechnology

Volumn 45, Issue 1-2, 1996, Pages 94-101

  Sakamoto, S ^a   Terada, I ^a   Lee, Y C ^b   Uehara, K ^a   Matsuzawa, H ^b   Iijima, M ^a  

^a JAPAN TOBACCO INC   (Japan)

^b UNIVERSITY OF TOKYO   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

AQUALYSIN 1; GLYCEROL; PROTEINASE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIUM CULTURE; CONTROLLED STUDY; DNA SEQUENCE; ENZYME SYNTHESIS; ESCHERICHIA COLI; MOLECULAR CLONING; NONHUMAN; THERMUS AQUATICUS;

BACTERIOPHAGE T7; BASE SEQUENCE; CLONING, MOLECULAR; CULTURE MEDIA; DNA, BACTERIAL; ENZYME INDUCTION; ESCHERICHIA COLI; GENES, BACTERIAL; GENETIC VECTORS; ISOPROPYL THIOGALACTOSIDE; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; SERINE ENDOPEPTIDASES; THERMUS; TRANSFORMATION, GENETIC;

EID: 0029966471     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002530050654     Document Type: Article

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