Centrifugal recovery and dissolution of recombinant Gly-IGF-II inclusion-bodies: The impact of feedrate and re-centrifugation on protein yield

Bioseparation

Volumn 6, Issue 3, 1996, Pages 185-192

  Wong, H H ^a   O'Neill, B K ^a   Middelberg, A P J ^a  

^a UNIVERSITY OF ADELAIDE   (Australia)

Author keywords

centrifugation; downstream processing; inclusion body; process interactions; recombinant protein

Indexed keywords

CELLS; CENTRIFUGATION; CENTRIFUGES; DISSOLUTION; ENZYMES; OPTIMIZATION; SOLUBILITY;

CENTRIFUGAL RECOVERY; INCLUSION BODIES; INSULIN LIKE GROWTH FACTOR; RE CENTRIFUGATION;

PROTEINS;

RECOMBINANT PROTEIN; SOMATOMEDIN B;

ARTICLE; BIOREACTOR; BIOSYNTHESIS; BIOTECHNOLOGY; CELL INCLUSION; CENTRIFUGATION; CHEMISTRY; ESCHERICHIA COLI; EVALUATION; FERMENTATION; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY;

BIOREACTORS; BIOTECHNOLOGY; CENTRIFUGATION; ESCHERICHIA COLI; EVALUATION STUDIES; FERMENTATION; HUMANS; INCLUSION BODIES; INSULIN-LIKE GROWTH FACTOR II; RECOMBINANT PROTEINS;

ESCHERICHIA COLI;

EID: 0030175128     PISSN: 0923179X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (2)

1. Collis M A, O'Neill, B K and Middelberg, A P J (1995). Thermal deactivation affects disruption of Escherichia coli. Biotechnol. Tech., 9: 91-94.
2. Hoare M and Dunnill P (1989). Biochemical engineering challenges of purifying useful proteins, Phil. Trans. R. Soc. Lond. B 324: 497-507.