Single-molecule detection with a two-photon fluorescence microscope with fast-scanning capabilities and polarization sensitivity

Optics Letters

Volumn 24, Issue 24, 1999, Pages 1832-1834

  Farrer, Richard A ^a   Previte, Michael J R ^a   Olson, Christopher E ^a   Peyser, Lynn Anne ^a   Fourkas, John T ^a   So, Peter T C ^b  

^a BOSTON COLLEGE   (United States)

^b Massachusetts Institute of Technology Cambridge   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0000531596     PISSN: 01469592     EISSN: None     Source Type: Journal    
DOI: 10.1364/OL.24.001832     Document Type: Article

References (9)

1. T. Basché, W. E. Moerner, M. Orrit, and U. Wild, eds., Single Molecule Optical Detection, Imaging and Spectroscopy (Verlag-Chemie, Weinheim, Germany, 1997).
2. W. E. Moeraer and M. Orrit, Science 283, 1670 (1999).
3. J. Mertz, C. Xu, and W. W. Webb, Opt. Lett. 20, 2532 (1995).
4. E. J. Sánchez, L. Novotny, G. R. Holtom, and X. S. Xie, J. Phys. Chem. A 101, 7019 (1997).
5. M. K. Lewis, P. Wolanin, A. Gafni, and D. G. Steel, Opt. Lett. 23, 1111 (1998).
6. M. Sonnleitner, G. J. Schütz, and Th. Schmidt, Chem. Phys. Lett. 300, 221 (1999).
7. W. L. Peticolas, J. P. Goldsborough, and K. E. Reickhoff, Phys. Rev. Lett. 10, 43 (1963).
8. In some confocal microscopes the collected fluorescence is reflected back off the scanning mirrors and thus can be focused to a constant position as the excitation position is scanned. However, this scheme is somewhat lossy, and the angle-dependent depolarization of the scanning mirrors would preclude the use of the polarization-senstive detection system described here.
9. P. T. C. So, T. French, W. M. Yu, K. M. Berland, C. Y. Dong, and E. Gratton, in Fluorescence Imaging Spectroscopy and Microscopy, X. F. Wang and B. Herman, eds. (Wiley, New York, 1996), pp. 351-374.