Cloning and expression of the porcine obese gene

Animal Biotechnology

Volumn 8, Issue 2, 1997, Pages 191-206

  Bidwell, Christopher A ^a   Ji, Shaoquan ^a,b   Frank, G Robert ^a,b   Cornelius, Steven G ^a,b   Willis, Gawain M ^a,b   Spurlock, Michael E ^a,b  

^a Purdue University ^*  (United States)

^b Purina Mills LLC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0000486969     PISSN: 10495398     EISSN: None     Source Type: Journal    
DOI: 10.1080/10495399709525882     Document Type: Article

References (18)

1. Briley, G.P. and C.A. Bidwell, 1994. Construction of a cosmid library using agarose embedded DNA. Biotechniques 17:278-280.
2. Campfield, L. A., F. J. Smith, Y. Guisez, R. Devos and P. Burn. 1995. Recombinant mouse ob protein: Evidence for a peripheral signal linking adiposity and central neural networks. Science 269:546-549.
3. Chomczynski, P., and N. Sacchi. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:156-159.
4. Frederich, R.C., A. Hamann, S. Anderson, B. Lollmann, B.B. Lowell, and J.S. Flier. 1995. Leptin levels reflect body lipid content in mice: Evidence for diet-induced resistance to leptin action. Nature Medicine 1:1311-1314.
5. Gong, D.-W., S. Bi, R.E. Pratley and B.D. Weintraub. 1996. Genomic structure and promoter analysis of the human obese gene. J. Biol. Chem. 271:3971-3974.
6. Halaas, J.L., K.S. Gajiwala, M. Maffei, S.L. Cohen, B.T. Chait, D. Rabinowitz, R.L. Lallone, S.K. Burley and J.M. Friedman. 1995. Weight-reducing effects of the plasma protein encoded by the obese gene. Science 269:543-546.
7. Hwang, C.-S., S. Mandrup, O.A. MacDougald, D.E. Geiman and M.D. Lane. 1996. Transcriptional activation of the mouse obese (ob) gene by CCAAT/enhancer binding protein α. Proc. Natl. Acad. Sci. U.S.A. 93:873-877.
8. Kozak, M. Interpreting cDNA sequences: some insights from studies on translation. Mammalian Genome 7: 563-574.
9. Loh, E.Y., J.F. Elliot, S. Cwirlia, L.L. Lanier, and M.M. Davis. 1989. Polymerase chain reaction with single sided specificity: Analysis of T-cell receptor δ chain. Science 243: 217-220.
10. Malik, K. F., and W. S. Young, III. 1996. Localization of binding sites in the central nervous system for leptin (OB protein) in normal, obese (ob/ob), and diabetic (db/db) C57BL/6J mice. Endocrinology 137:1497-1500.
11. Neuenschwander, S., G. Rettenberger, E. Meijerink, H. Jorg, and G. Stranzinger. 1996. Partial characterization of porcine obesity gene (OBS) and its localization to chromosome 18 by somatic cell hybrids. Animal Genetics 27: 275-278.
12. Pellymounter, M.A., M.J. Cullen, M.B. Baker, R. Hecht, D. Winters, T. Boone and F. Collins. 1995. Effects of the obese gene product on body weight regulation in ob/ob mice. Science 269:540-543.
13. Sambrook, J., E.F. Fritsch, Manniatis T. 1989 Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press. New York.
14. Sasaki, S., A.C. Clutter, and D. Pomp. 1996. Assignment of the porcine obese (leptin) gene to chromosome 18 by linkage analysis of a new PCR based polymorphism. Mammalian Genome 7: 471-472.
15. Schwartz, M.W., D.G. Baskin, T.R. Bukowski, J.L. Kuijper, D. Foster, G. Lasser, D.E. Prunkard, D. Porte, Jr., S.C. Woods, R.J. Seeley, and D.S. Weigle. 1996. Specificity of leptin action on elevated blood glucose levels and hypothalamic neuropeptide Y gene expression in ob/ob mice. Diabetes 45:531-535.
16. Stephens, T.W., M. Baslnski, P.K. Bristow, J.M. Bue-Valleskey, S.G. Burgett, L. Craft, J. Hale, J. Hoffman, H.M. Halung, A. Kriauclunas, W. MacKellar, P.R. Rostack, Jr., B. Schoner, D. Smith, F.C. Tinsley, X. Zhang, and M. Helman. 1995. The role of neuropeptide Y in the antiobesity action of the obese gene product. Nature 377:530-532.
17. Zhang, Y., R. Proenca, M. Maffei, M. Barone, L. Leopold, and J.M. Freidman. 1994. Positional cloning of the mouse obese gene and its human homologue. Nature 372: 425-432.
18. Zheng, D., J.P. Jones, S.J. Usala, and G.L. Dohm. 1996. Differential expression of ob mRNA in rat adipose tissues in response to insulin. Biochem. Biophys. Res. Comm. 218:434-437.