Distribution of viable and non-viable bacteria in the gastrointestinal tract of gnotobiotic and conventional rats

Microbial Ecology in Health and Disease

Volumn 11, Issue 4, 1999, Pages 218-225

  Kleessen, Brigitta ^a   Noack, Jutta ^a   Blaut, Michael ^a  

^a GERMAN INSTITUTE OF HUMAN NUTRITION   (Germany)

Author keywords

Conventional rat; Gnotobiotic rat; In situ hybridization; Intestinal microflora; Viability staining

Indexed keywords

EID: 0000395173     PISSN: 0891060X     EISSN: None     Source Type: Journal    
DOI: 10.1080/08910609908540831     Document Type: Article

References (27)

1. Finegold SM, Sutler VL, Mathisen GE. Normal indigenous flora. In: Hentges DJ, ed. Human intestinal microflora in health and disease. New York: Academic Press, Inc, 1983: 3-31.
2. Mitsuoka T. Recent trends in research on intestinal flora. Bifidobacteria Microflora 1982; 1: 3-24.
3. Welling GW, Elfferich P, Raangs GC, Wildeboer-Veloo ACM, Jansen GJ, Degener JE. 16S ribosomal RNA-targeted oligonucleotide probes for monitoring of intestinal tract bacteria. Scand J Gastroenterol, 1997; 32 Suppl. 222: 17-19.
4. Raymond L, Kepner JR, Pratt JR. Use of fluochromes for direct enumertion of total bacteria in environmental samples: past and present. Microbiol Rev. 1994; 58: 603-615.
5. Roth BL, Poot M, Yue ST, Millard PJ. Bacterial viability and antibiotic susceptibility testing with Sytox green nucleic acid stain. Appl Environ Microbiol. 1997; 63: 2421-31.
6. Tsuji T, Kawasaki Y, Takeshima S, Sekiya T, Tanaka S. A new fluorescence staining assay for visualizing living microoganisms in soil. Appl Environ Microbiol. 1995; 61: 3415-21.
7. Jacobsen CN, Rasmussen J, Jakobsen M. Viability staining and flow cytometric detection of Listeria monocytogenes. J Microbiol Methods. 1997; 28: 35-43.
8. Lebaron P, Parthuisot N, Catale P. Comparison of blue nucleic acid dyes for flow cytometric enumeration of bacteria in aquatic systems. Appl Environ Microbiol. 1998; 64: 1725-30.
9. Molecular Probes, Inc. 1996. LIVE/DEAD BacLight® bacterial viability kit (L-7007) instruction manual with appendix. Molecular Probes, Inc., Eugene, Oreg.
10. Jansen GJ, Wilkinson MHF, Deddens B, Van der Waaij D. Characterization of human faecal flora by means of an improved fluoro-morphometrical method. Epidemiol Infect. 1993; 111: 265-72.
11. Amann RI, Springer N, Ludwig W, Görtz H-D, Schleifer K-H. Identification in situ and phylogeny of uncultured bacterial endosymbionts. Nature 1991; 351: 161-4.
12. Amann RI, Ludwig W, Schleifer K-H. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol Rev. 1995; 59: 143-69.
13. Franks AH, Harmsen HJM, Raangs GC, Jansen GJJ, Schut F, Welling GW. Variations of bacterial populations in human feces measured by fluorescent in situ hybridizatin with group-specific 16S rRNA-targeted oligonucleotide probes. Appl Environ Microbiol. 1998; 64: 3264-9.
14. DeLong EF, Wickham GS, Pace NR. Phylogenetic stains: ribosomal RNA-based probes for the identification of single cells. Science 1989; 243: 1360-3.
15. Manz W, Szewzyk U, Eriksson P, Amann RI, Schleifer K-H, Stenström T-A. In situ identification of bacteria in drinking water and adjoining biofilms by hybridization with 16S and 23S rRNA-directed fluorescent oligonucleotide probes. Appl Environ Microbiol. 1993; 59: 2293-8.
16. Kleessen B, Bunke H, Tovar K, Noack J, Sawatzki K. Influence of two infant formulas and human milk on the development of the faecal flora in newborn infants. Acta Paediatr. 1995; 84: 1347-56.
17. Roszak DB, Colwell RR. Survival strategies of bacteria in the natural environment. Microbiol Rev. 1987; 51: 365-79.
18. Cummings JH, Englyst HN. Fermentation in the human large intestine and the available substrates. Am J Clin Nutr. 1987; 45: 1243-55.
19. Lewis DL, Gattie DK. The ecology of quiescent microbes. ASM News. 1991; 57: 27-32.
20. Schaechter M, Maaloe O, Kjeldgaard NO. Dependency on medium and temperature of cell size and chemical composition during balanced growth of Salmonella typhimurium. J Gen Microbiol. 1958; 19: 592-606.
21. Kaplan R, Apiron D. The fate of ribosomes in Escherichia coli cells starved for a carbon source. J Biol Chem. 1975; 250: 1854-63.
22. Flardh K, Cohen PS, Kjelleberg S. Ribosomes exist in large excess over the apparent demand for protein synthesis during carbon starvation in marine Vibrio sp. Strain CCUG 15956. J Bacteriol. 1991; 174: 6780-8.
23. Poulsen LK, Licht TR, Rang C, Krogfelt KA, Molin S. Physiological state of Escherichia coli BJ4 growing in the large intestine of streptomycin-treated mice. J Bacteriol. 1995; 177: 5840-5.
24. Krogfelt KA, Poulsen LK, Molin S. Identification of coccoid Escherichia coli BJ4 in the large intestine of streptomycin-treated mice. Infect Immun. 1993; 61: 5029-34.
25. Poulsen LK, Ballard G, Stahl DA. Use of rRNA fluorescence in situ hybridization for measuring the activity of single cells in young and established biofilms. Appl Environ Microbiol. 1993; 59: 1354-60.
26. Langendijk PS, Schut F, Jansen GJ, Raangs GC, Kamphuis CR, Wilkinson MHF, Welling GW. Quantitative fluorescence in situ hybridization of Bifidobacterium spp. with genus-specific 16S rRNA-targeted probes and its application in faecal samples. Appl Environ Microbiol. 1995; 61: 3069-75.
27. Macfarlane GT, Macfarlane S. Factors affecting fermentation reactions in the large bowel. Proc Nut Soc. 1993; 52: 367-73.