Spectroscopic evidence for inner-sphere coordination of metal ions to the active site of a hammerhead ribozyme

Journal of the American Chemical Society

Volumn 120, Issue 18, 1998, Pages 4518-4519

  Cunningham, Lynette A ^a   Li, Jing ^a   Lu, Yi ^a  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0000262982     PISSN: 00027863     EISSN: 15205126     Source Type: Journal    
DOI: 10.1021/ja973251p     Document Type: Letter

References (49)

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43. thio is similar in magnitude which may indicate that the coordination geometry is not particularly different between the two systems.
44. thio possesses the correct structural configuration for cleavage activity (see footnotes 23 and 26)
45. thio does not result in any significant cleavage above the background level
46. 2, an immediate appearance of the broad 300-nm band was observed
47. thio and 40 μM ribozyme (in. 200 mM PIPES)
48. thio remains constant from 5 to 50 equiv of Hg(II). However, the cleavage activity of Sp ESthio is inhibited by Hg(II) above 5 equiv (0.5 μ\u;M), probably due to disruption of the active site
49. 2 is completely inactive toward C-5 mercuration and (b) the rate of mercuration of heteropolynucleotides using HgOAc, the best reagent for C-5 mercuration, is considerably slower than that of mononucleotides and requires high temperature and prolonged incubation