Photodamage to human RPE cells by A2-E, a retinoid component of lipofuscin

Investigative Ophthalmology and Visual Science

Volumn 41, Issue 8, 2000, Pages 2303-2308

  Schütt, Florian ^c   Davies, Sallyanne ^c   Kopitz, Jürgen ^a   Holz, Frank G ^b   Boulton, Michael E ^c  

^a MANCHESTER ROYAL EYE HOSPITAL   (United Kingdom)

^b UNIVERSITY OF HEIDELBERG   (Germany)

^c NONE

Author keywords

[No Author keywords available]

Indexed keywords

ACRIDINE ORANGE; LIPOFUSCIN; LOW DENSITY LIPOPROTEIN; N RETINYLIDENE N RETINYLETHANOLAMINE; RETINOID; UNCLASSIFIED DRUG;

ARTICLE; BIOACCUMULATION; CELL CULTURE; CELL VIABILITY; CONTROLLED STUDY; HUMAN; HUMAN CELL; LIGHT; LYSOSOME; PATHOGENESIS; PH; PHOTOREACTIVITY; PHOTOTOXICITY; PRIORITY JOURNAL; RETINA MACULA AGE RELATED DEGENERATION; RETINA PIGMENT EPITHELIOPATHY; STARGARDT DISEASE;

ACRIDINE ORANGE; CELL SURVIVAL; CELLS, CULTURED; FLOW CYTOMETRY; FLUORESCENT DYES; HUMANS; LIGHT; LIPOFUSCIN; LYSOSOMES; MICROSCOPY, FLUORESCENCE; PIGMENT EPITHELIUM OF EYE; RETINAL PIGMENTS; RETINOIDS;

EID: 0000117096     PISSN: 01460404     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (38)

1. Sohal RS, ed. Age Pigments. Amsterdam: Elsevier, 1981.
2. Brizzee KR, Ordy JM. Cellular features, regional accumulation, and prospects of modification of age pigments in mammals. In: Sohal RS, ed. Age Pigments. Amsterdam: Elsevier; 1981:101-154.
3. Boulton M, Marshall J. Effects of increasing numbers of phagocytic inclusions on human retinal pigment epithelial cells in culture: a model for aging BrJ Ophthalmol. 1986;70:808-815.
4. Dorey CK, Wu G, Ebenstein D, Garsd A, Weiter JJ. Cell loss in the aging retina: relationship to lipofuscin accumulation and macular degeneration. Invest Ophthalmol Vis Sci. 1989;30:1691-1699.
5. Herman ER. Retinal pigment epithelium: lysosomal enzymes and aging. Br J Ophthalmol. 1994;78:82-83.
6. Holz FG, Bellmann C, Margaritidis M, Schütt F, Otto TP, Völcker HE. Patterns of increased in vivo autofluorescence in the junctional zone of geographic atrophy of the retinal pigment epithelium associated with age-related macular degeneration. Graefes Arch Clin Exp Ophthalmol. 1999;237:145-152.
7. Kennedy CJ, Rakoczy PE, Constable IJ. Lipofuscin of the retinal pigment epithelium: a review. Eye. 1995;9:763-771.
8. Bird AC. Age-related macular disease. Br J Ophthalmol. 1996;80: 1-2.
9. Allikmets R, Shroyer NF, Singh N, et al. Mutation of the Stargardt disease gene (ABCR) in age-related macular degeneration. Science. 1997;277:1805-1807.
10. Frangieh GT, Green WR, Fine SL. A histopathologic study of Best's macular dystrophy. Arch Ophthalmol. 1982;100:1115-1121.
11. Weingeist TA, Kohrin JL, Watz KE. Histopathology of Best's macular dystrophy Arch Ophthalmol. 1982;100:1108-1114.
12. Lopez PF, Maumenee IH, de la Cruz Z, Green WR. Autosomal-dominant fundus flavimaculatus: clinicopathologic correlation. Ophthalmology. 1990;97:798-809.
13. Eagle RC, Lucier AC, Bernadino VB, Janoff M. Retinal pigment epithelial abnormalities in fundus flavimaculatus: a light and electron microscopical study. Ophthalmology. 1980;87:1189-2000.
14. Steinmetz RL, Gamer A, Maguire JI, Bird AC. Histopathology of incipient fundus flavimaculatus. Ophthalmology. 1991;98:953-956.
15. Feeney-Burns L, Eldred GE The fate of the phagosome: conversion to age pigment and impact in human retinal pigment epithelium. Trans Ophthalmol Soc UK. 1983;103:416-421.
16. Katz ML, Gao CL, Rice LM. Formation of lipofuscin-like fluorophores by reaction of retinal with photoreceptor outer segments and liposomes. Mech Ageing Dev. 1996;92:159-174.
17. Rozanowska M, Jarvis-Evans J, Korytowski W, Boulton M, Burke JM, Sarna T. Blue light induced reactivity of retinal age pigment: in vitro generation of oxygen reactive species. J Biol Chem. 1995; 270:18825-18830.
18. Rozanowska M, Wessels J, Boulton M, et al Blue light-induced singlet oxygen generation by retinal lipofuscin in non-polar media Free Radic Biol Med. 1998;24:1107-1112.
19. Wassell J, Davies S, Bardsley W, Boulton M. The photoreactivity of the retinal age pigment lipofuscin. J Biol Chem. 1999;274:23828-23832.
20. Davies S, Mulroy L, McGarvey D, Truscott TG, Boulton M. The phototoxicity of lipofuscin [ARVO Abstract]. Invest Ophthalmol Vis Sci. 1998;39:8129. Abstract nr 601.
21. Feeney-Burns L, Berman ER, Rothman H. Lipofuscin of human retinal pigment epithelium. Am J Ophthalmol. 1980;90:783-791.
22. Gaillard ER, Atherton SJ, Eldred G, Dillon J. Photophysical studies on human retinal lipofuscin. Photochem Photobiol. 1995;61:448-453.
23. Eldred GE, Katz ML. The lipid peroxidation theory of lipofuscino-genesis cannot yet be confirmed. Free Radic Biol Med. 1991;10: 445-447.
24. Eldred GE, Lasky MR. Retinal age-pigments generated by self-assembling lysosomotropic detergents. Nature. 1993;361:724-726.
25. Eldred GE. Age pigment structure. Nature. 1993;364:396.
26. Parish CA, Hashimoto M, Nakanishi K, Dillon J, Sparrow J. Isolation and one-step preparation of A2-E and iso-A2-E fluorophores from human retinal pigment epithelium. Proc Natl Acad Sci USA. 1998; 95:14609-14613.
27. Holz FG, Schuett F, Kopitz J, et al. Inhibition of lysosomal degradative functions in RPE cells by a retinoid component of lipofuscin. Invest Ophthalmol Vis Sci. 1999;40:737-743.
28. Dillon J, Gaillard ER, Bilski P, Chignell CF, Reszka KJ. The photochemistry of the retinoids as studied by steady-state and pulsed methods. Photochem Photobiol. 1996;63:680-685.
29. Reinboth JJ, Gautschi K, Munz K, Eldred GE, Reme CE. Lipofuscin in the retina: quantitative assay for an unprecedented autofluorescent compound (pyridinium bis-retinoid, A2-E) of ocular age pigment. Exp Eye Res. 1997;65:639-43.
30. Eldred GE, Katz ML. Fluorophores of the human retinal pigment epithelium: separation and spectral characterization. Exp Eye Res. 1988;47:71-86.
31. Hayes KC, Lindsey S, Stephan ZF, Brecker D. Retinal pigment epithelium possesses both LDL and scavenger receptor activity. Invest Ophthalmol Vis Sci. 1989;30:225-232.
32. Rudel L, Lee J, Morris M, Fetts J. Characterization of plasma lipoproteins separated and purified by agarose-column chromatography. Biochem J. 1974;139:89-95.
33. Boulton M, Marshall J, Mellerio J. Retinitis pigmentosa: a preliminary report on tissue culture studies of retinal pigment epithelial cells from eight affected human eyes. Exp Eye Res. 1983;37:307-313.
34. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983;65:55-63.
35. Cubeddu R, Taroni P, Hu DN, Sakai N, Nakanishi K, Roberts JE. Photophysical studies of A2-E, putative precursor of lipofuscin, in human retinal pigment epithelial cells. Photochem Photobiol. 1999;70:172-5.
36. Rozanowska M, Sarna T, Land EJ, Truscott TG. Free radical scavenging properties of melanin interaction of eu-and pheo-melanin models with reducing and oxidising radicals. Free Radic Biol Med. 1999;26:518-525.
37. Dorey CK, Delori FC, Akeo K. Growth of cultured RPE and endothelial cells is inhibited by blue light but not green or red light. Curr Eye Res. 1990;9:549-59.
38. Weng J, Mata NL, Asarian SM, Tzekov RT, Birch DG, Travis GH. Insights into the function of Rim protein in photoreceptors and etiology of Stargardt's disease from the phenotype in aber knockout mice. Cell. 1999;98:13-23.