Lipofuscin in the retina: Quantitative assay for an unprecedented autofluorescent compound (pyridinium bis-retinoid, A2-E) of ocular age pigment

Experimental Eye Research

Volumn 65, Issue 5, 1997, Pages 639-643

  Reinboth, J J ^a   Gautschi, K ^a   Munz, K ^a   Eldred, G E ^b   Reme Ch E ^a  

^a UNIVERSITY HOSPITAL ZURICH   (Switzerland)

^b Dept of Clinical Chemistry ^*

Author keywords

fluorophores; high pressure liquid chromatography (HPLC); lipofuscin; N retinylidene N retinylethanolamin e (A2 E); pyridinium bis retinoid; retinal pigment epithelium

Indexed keywords

LIPOFUSCIN; RETINOID;

ACCURACY; AGE; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MALE; NONHUMAN; PIGMENT EPITHELIUM; PRIORITY JOURNAL; QUANTITATIVE ASSAY; RAT;

EID: 0030663593     PISSN: 00144835     EISSN: None     Source Type: Journal    
DOI: 10.1006/exer.1997.0367     Document Type: Article

References (17)

1. Berg, K. Moan, J. 1994, Lysosomes as photochemical targets. Int. J. Cancer, 59, 814, 22.
2. Burns, R. P. Feeney-Burns, L. 1980, Clinico-morphologic correlations of drusen of Bruch's membrane. Trans. Am. Ophthalmol. Soc. 78, 206, 25.
3. Colombo, J. P. 1978, Zuverlässigkeit von Labormethoden und qualitätskontrolle. Klinische chemie. Theorie, Praxis, Interpretation, 57, 67, S. Karger Verlag, Basel: Switzerland.
4. Donaldson, H. H. 1924, Growth of parts and systems of the body in weight. The rat. Data and reference tables for the albino rat and Norway rat, Donaldson, H. H. 181, 298, Philadelphia, GA; U.S.A.
5. Eldred, G. E. Katz, M. L. 1988, Fluorophores of the human retinal pigment epithelium: separation and spectral characterization. Exp. Eye Res. 47, 71, 86.
6. Eldred, G. E. 1993a, Retinoid reaction products in age related degeneration. Retinal degeneration: clinical and laboratory applications, Hollyfield, J. G.LaVail, M. M.Anderson, R. E. 15, 25, Plenum Press, New York, U.S.A.
7. Eldred, G. E. Lasky, M. R. 1993, Retinal age pigments generated by self-assembling lysosomotropic detergents. Nature, 361, 724, 6.
8. Eldred, G. E. 1995, Lipofuscin fluorophore inhibits lysosomal protein degradation and may cause early stages of macular degeneration. Gerontol. 41, 15, 28.
9. Feeney-Burns, L. Eldred, G. E. 1983, The fate of the phagosome: conversion to 'age pigment' and impact in human retinal epithelium. Trans. Ophthalmol. Soc. U.K. 103, 416, 21.
10. Feeney-Burns, L. Hilderbrand, E. S. Eldridge, S. 1984, Aging human RPE: morphometric analysis of macular, equatorial and peripheral cells. Invest. Ophthalmol. Vis. Sci. 25, 195, 200.
11. Gaillard, E. R. Atherton, S. J. Eldred, G. E. Dillon, J. 1995, Photophysical studies on human retinal lipofuscin. Photochem. Photobiol. 61, 448, 53.
12. Hogan, M. J. 1972, Role of the retinal pigment epithelium in macular disease. Trans. Am. Acad. Ophthalmol. Otolaryngol. 76, 64, 80.
13. Katz, M. L. 1989, Incomplete proteolysis may contribute to lipofuscin accumulation in the retinal pigment epithelium. Adv. Exp. Med. Biol. 266, 109, 16.
14. Kopitz, J. Monahan, D. Stogsdill, P. L. Cantz, M. Eldred, G. E. 1996, Evidence that an unprecedented vitamin A derivative may underlie the leading cause of age-related blindness. Am. J. Pathol. 148, 1, 7.
15. Matsumoto, Y. Watanage, T. Suga, T. Fujitani, H. 1989, Inhibitory effects of quarternary ammonium compounds on lysosomal degradation of endogenous proteins. Chem. Pharm. Bull. 37, 516, 18.
16. Sakai, N. Decatur, J. Nakanishi, K. Eldred, G. E. 1996, Ocular age pigment 'A2-E': an unprecedented pyridinium bisretinoid. J. Am. Chem. Soc. 118, 1559, 60.
17. Seglen, P. O. 1983, Inhibitors of lysosomal function. Meth. Enzymol. 96, 737, 64.