Biomolecular recognition using submicron laser lithography

Applied Physics Letters

Volumn 73, Issue 3, 1998, Pages 417-419

  Shivashankar, G V ^a   Libchaber, A ^a  

^a ROCKEFELLER UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0000048116     PISSN: 00036951     EISSN: None     Source Type: Journal    
DOI: 10.1063/1.121852     Document Type: Article

References (18)

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11. 2) are prepared by standard sputtering (BAL-TEC MED 020) in argon atmosphere, the target being kept at room temperature. Rate of deposition 5 Å/s. The thickness, 50 Å, is measured in situ using a quartz monitor.
12. Optical path: a near infrared laser diode (SDL Inc.) is collimated to a circular beam of 8 mm diameter. Two telescopic lenses (100 mm focal length) are used for beam steering. To generate patterns smaller than 30 μm, beam steering is used. For larger patterns, the sample stage on which the coverslip is mounted (not shown in Fig. 1) is translated.
13. 11beads/μl (volume 10 μl) are used, concentration depletion at the etching front during lithography is negligible.
14. Lithography: Control parameters of the lithographic process are: laser power, spot size, particle size and concentration, rate of inscription. As we draw a line by moving the sample stage (or beam steering), we do not automatically correct for small z shift of the objective. This leads to slight inhomogeneity of the bead deposition. To pattern biomolecules, a near infrared laser is suitable. At this wavelength there is minimal damage to the biomolecules. Any substrate may work as long as there is localized heating and a solution with particle suspension.
15. G. V. Shivashankar and A. Libchaber, Appl. Phys. Lett. 71, 3727 (1997).
16. 12 particles.
17. 12beads) and incubated at room temperature for 10 min. The construct is identical for sequence A and sequence B.
18. Detection: Complementary sequences of both oligonucleotides (0.05 mg/ml in TE buffer, 20 μl volume) are introduced in the sample cell. The hybridization reaction is carried out for 1 h, at room temperature. After rinsing, detection is done using standard fluorescence microscopy and image intensifier (Hamamatsu).