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Volumn 108, Issue 46, 2004, Pages 18046-18052

Surface-enhanced raman difference between human insulin and insulin lispro detected with adaptive nanostructures

Author keywords

[No Author keywords available]

Indexed keywords

HUMAN INSULIN; NANOSTRUCTURE MORPHOLOGY; PROTEIN DEPOSITION;

EID: 9944227316     PISSN: 15206106     EISSN: None     Source Type: Journal    
DOI: 10.1021/jp047254h     Document Type: Article
Times cited : (101)

References (72)
  • 34
    • 0031037501 scopus 로고    scopus 로고
    • Nie, S.; Emory, S. R. Science 1997, 275, 1102; J. Phys. Chem. B 1998, 102, 493.
    • (1997) Science , vol.275 , pp. 1102
    • Nie, S.1    Emory, S.R.2
  • 35
    • 0031647707 scopus 로고    scopus 로고
    • Nie, S.; Emory, S. R. Science 1997, 275, 1102; J. Phys. Chem. B 1998, 102, 493.
    • (1998) J. Phys. Chem. B , vol.102 , pp. 493
  • 56
    • 9944259715 scopus 로고    scopus 로고
    • note
    • 2 (10 nm) followed by an Ag layer (8 nm) deposited at a rate of 0.05 nm/s. The fabricated films were characterized by field emission scanning electron microscopy (FE SEM), atomic force microscopy (AFM), and a Lambda 35 spectro-photometer (Perkin-Elmer) equipped with a Labsphere. High-resolution FE SEM images were obtained through MAS Inc. (Raleigh, NC). AFM images were acquired with a Dimension 3100 (Dl Veeco) using a 10 nm Si tip for measuring height profiles of our samples.
  • 57
    • 9944247980 scopus 로고    scopus 로고
    • note
    • This experiment emphasizes the importance of chemical content versus surface tension of drying protein droplets in restructuring at the nanoscale. Note that proteins of other kinds may cause restructuring even in water solution.
  • 64
    • 9944231832 scopus 로고    scopus 로고
    • note
    • A profilometer stylus may scratch soft material, which results in poor accuracy in profile measurements. To avoid this problem, the lowest possible stylus force (5 mg) was applied to our samples. We were able to detect a profile contrast of about 10 for insulin spots. A representative profile is shown in ref 65. Since an area of the spot ring is relatively small, an estimate for the profile contrast in the range 5-12 gives good enough accuracy for the ratio between the local protein density in the central part and average one, which is about 0.4 × 0.1.
  • 65
    • 9944230839 scopus 로고    scopus 로고
    • note
    • -1.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.