Tri1 in Fusarium graminearum Encodes a P450 Oxygenase

Applied and Environmental Microbiology

Volumn 70, Issue 4, 2004, Pages 2044-2051

  McCormick, S P ^a,c   Harris, L J ^b   Alexander, N J ^a   Ouellet, T ^b   Saparno, A ^b   Allard, S ^b   Desjardins, A E ^a  

^a NATIONAL CENTER FOR AGRICULTURAL UTILIZATION RESEARCH   (United States)

^b AGRICULTURE AND AGRI FOOD CANADA   (Canada)

^c AGRICULTURAL RESEARCH SERVICE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CONTAMINATION; CROPS; DNA; ENZYME KINETICS; GENES; PLANT CELL CULTURE;

GENE DISRUPTANTS; GENE ENCODING;

MICROBIOLOGY;

15 ACETYLVOMITOXIN; 3 DEACETYLCALONECTRIN; CALONECTRIN; CYTOCHROME P450; CYTOCHROME P450 OXYGENASE; MYCOTOXIN; NATURAL PRODUCT; OXYGENASE; TRICHOTHECENE DERIVATIVE; UNCLASSIFIED DRUG; VOMITOXIN;

GENE EXPRESSION;

AMINO ACID SEQUENCE; ARTICLE; DNA LIBRARY; DNA SEQUENCE; FOOD CONTAMINATION; FUNGAL GENE; FUNGUS CULTURE; FUSARIUM GRAMINEARUM; FUSARIUM SPOROTRICHIOIDES; GENE; GENE DISRUPTION; GENE EXPRESSION; GENE FUNCTION; GIBBERELLA; GIBERRELLA ZEAE; GRAIN; LH1 GENE; MAIZE; NONHUMAN; NORTH AMERICA; NUCLEOTIDE SEQUENCE; OXYGENATION; SEQUENCE HOMOLOGY; TRI1 GENE; WHEAT;

BASE SEQUENCE; CYTOCHROME P-450 ENZYME SYSTEM; DNA PRIMERS; DNA, FUNGAL; EXPRESSED SEQUENCE TAGS; FUSARIUM; GENE TARGETING; GENES, FUNGAL; MODELS, BIOLOGICAL; MOLECULAR SEQUENCE DATA; PHENOTYPE; PLANT DISEASES; TRICHOTHECENES;

FUNGI; FUSARIUM; FUSARIUM SPOROTRICHIOIDES; GIBBERELLA; GIBBERELLA ZEAE; MYXOGASTRIA; TRITICUM AESTIVUM; ZEA MAYS;

EID: 9944226571     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.70.4.2044-2051.2004     Document Type: Article

References (30)

1. Bowden, R. L., and J. F. Leslie. 1992. Nitrate-nonutilizing mutants of Gibberella zeae (Fusarium graminearum) and their use in determining vegetative compatibility. Exp. Mycol. 16:308-315.
2. Brown, D. W., S. P. McCormick, N. J. Alexander, R. H. Proctor, and A. E. Desjardins. 2002. Inactivation of a cytochrome P-450 is a determinant of trichothecene diversity in Fusarium species. Fungal Genet. Biol. 36:224-233.
3. Brown, D. W., S. P. McCormick, N. J. Alexander, R. H. Proctor, and A. E. Desjardins. 2001. A genetic and biochemical approach to study trichothecene diversity in Fusarium sporotrichioides and F. graminearum. Fungal Genet. Biol. 32:121-133.
4. Desjardins, A. E., R. H. Proctor, G. Bai, S. P. McCormick, G. Shaner, G. Beuchley, and T. M. Hohn. 1996. Reduced virulence of trichothecene-non-producing mutants of Gibberella zeae in wheat field tests. Mol. Plant-Microbe Interact. 9:775-781.
5. Diatchenko, L., Y.-F. C. Lau, A. P. Campbell, A. Chenchik, F. Moqadam, B. Huang, S. Lukyanov, K. Lukyanov, N. Gurskaya, E. D. Sverdlov, and P. D. Siebert. 1996. Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries. Proc. Natl. Acad. Sci. USA 93: 6025-6030.
6. Helliwell, C. A., P. M. Chandler, A. Poole, E. S. Dennis, and W. J. Peacock. 2001. The CYP88A cytochrome P450, ent-kaurenoic acid oxidase, catalyzes three steps of the gibberellin biosynthesis pathway. Proc. Natl. Acad. Sci. USA 98:2065-2070.
7. Hesketh, A. R., L. Gledhill, B. W. Bycroft, P. M. Dewick, and J. Gilbert. 1993. Potential inhibitors of trichothecene biosynthesis in Fusarium culmorum epoxidation of a trichodiene derivative. Phytochemistry 32:93-104.
8. Hohn, T. M., R. Krishna, and R. H. Proctor. 1999. Characterization of a transcriptional activator controlling trichothecene toxin biosynthesis. Fungal Genet. Biol. 26:224-235.
9. Jurgenson, J. E., R. L. Bowden, K. A. Zeller, J. F. Leslie, N. J. Alexander, and R. D. Plattner. 2002. A genetic map of Gibberella zeae (Fusarium graminearum). Genetics 160:1451-1460.
10. Kimura, M., I. Kaneko, M. Komiyama, A. Takasuki, H. Koshino, K. Yoneyama, and I. Yamaguchi. 1998. Trichothecene 3-O-acetyltransferase protects both the producing organism and transformed yeast from related mycotoxins. J. Biol. Chem. 273:1654-1661.
11. Kimura, M., T. Tokai, K. O'Donnell, T. J. Ward, M. Fujimura, H. Hamamoto, T. Shibata, and I. Yamaguchi. 2003. The trichothecene biosynthesis gene cluster of Fusarium graminearum F15 contains a limited number of essential pathway genes and expressed non-essential genes. FEBS Lett. 539:105-110.
12. Lee, T., D. W. Oh, H. S. Kim, J. Lee, Y. H. Kim, S. H. Yun, and Y. W. Lee. 2001. Identification of deoxynivalenol- and nivalenol producing chemotypes of Gibberella zeae by using PCR. Appl. Environ. Microbiol. 67: 2966-2972.
13. Lee, T., Y.-K. Han, K.-H Kim, S.-H Yun, and Y.-W. Lee. 2002. Tri13 and Tri7 determine deoxynivalenol- and nivalenol-producing chemotypes of Gibberella zeae. Appl. Environ. Microbiol. 68:2148-2154.
14. McCormick, S. P., and N. J. Alexander. 2002. Fusarium Tri8 encodes a trichothecene C-3 esterase. Appl. Environ. Microbiol. 68:2959-2964.
15. McCormick, S. P., and T. M. Hohn. 1997. Accumulation of trichothecenes in liquid cultures of a Fusarium sporotrichioides mutant lacking a functional trichothecene C-15 hydroxylase. Appl. Environ. Microbiol. 63:1685-1688.
16. McCormick, S. P., T. M. Hohn, and A. E. Desjardins. 1996. Isolation and characterization of Tri3, a gene encoding 15-O-acetyltransferase from Fusarium sporotrichioides. Appl. Environ. Microbiol. 62:353-359.
17. McCormick, S. P., N. J. Alexander, S. E. Trapp, and T. M. Hohn. 1999. Disruption of TRI101, the gene encoding trichothecene 3-O-acetyltransferase, from Fusarium sporotrichioides. Appl. Environ. Microbiol. 65:5252-5256.
18. Meek, I. B., A. W. Peplow, C. Ake, Jr., T. D. Phillips, and M. N. Beremand. 2003. Tri1 encodes the cytochrome P450 monooxygenase for C-8 hydroxylation during trichothecene biosynthesis in Fusarium sporotrichioides and resides upstream of another new Tri gene. Appl. Environ. Microbiol. 69: 1607-1613.
19. Miller, J. D., and B. A. Blackwell. 1986. Biosynthesis of 3-acetyldeoxynivalenol and other metabolites by Fusarium culmorum HLK 1503 in a stirred jar fermentor. Can. J. Bot. 64:1-5.
20. Nirenberg, H. I., and K. O'Donnell. 1998. New Fusarium species and combinations within the Gibberella fujikuroi species complex. Mycologia 90:434-458.
21. Peplow, A. W., I. B. Meek, M. C. Wiles, T. D. Phillips, and M. N. Beremand. 2003. Tri16 is required for esterification of position C-8 during trichothecene mycotoxin production by Fusarium sporotrichioides. Appl. Environ. Microbiol. 69:5935-5940.
22. Proctor, R. H., T. M. Hohn, and S. P. McCormick. 1995. Reduced virulence of Gibberella zeae caused by disruption of a trichothecene toxin biosynthetic gene. Mol. Plant-Microbe Interact. 8:593-601.
23. Rojas, M. C., P. Hedden, P. Gaskin, and B. Tudzynski. 2001. The P450-1 gene of Gibberella fujikuroi encodes a multifunctional enzyme in gibberellin biosynthesis. Proc. Natl. Acad. Sci. USA 98:5838-5843.
24. Sambrook, J., and D. W. Russell. 2001. Molecular cloning: a laboratory manual, 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
25. Sinha, R. C., and M. E. Savard. 1996. Comparison of immunoassay and gas chromatography methods for the detection of the mycotoxin deoxynivalenol in grain samples. Can. J. Plant Pathol. 18:233-236.
26. Stevens, R. R. 1974. Mycology guidebook, p. 703. University of Washington Press, Seattle.
27. Tilburn, J., S. Sarker, D. A. Widdick, E. A. Espeso, M. Orejas, J. Mungroo, M. A. Penalva, and H. N. Arst, Jr. 1995. The Aspergillus PacC zinc finger transcription factor mediates regulation of both acid- and alkaline-expressed genes by ambient pH. EMBO J. 14:779-790.
28. Trail, F., J.-R. Xu, P. San Miguel, R. G. Halgren, and H. C. Kistler. 2003. Analysis of expressed sequence tags from Gibberella zeae (anamorph Fusarium graminearum). Fungal Genet. Biol. 38:187-197.
29. Tudzynski, B., M. C. Rojas, P. Gaskin, P., and P. Hedden. 2002. The gibberellin 20-oxidase of Gibberella fujikuroi is a multifunctional monooxygenase. J. Biol. Chem. 277:21246-21253.
30. Turgeon, B. G., R. C. Garber, and O. C. Yoder. 1987. Development of a fungal transformation system based on selection of sequences with promoter activity. Mol. Cell. Biol. 17:3297-3305.