An erythroid and megakaryocytic common precursor cell line (B1647) expressing both c-mpl and erythropoietin receptor (Epo-R) proliferates and modifies globin chain synthesis in response to megakaryocyte growth and development factor (MGDF) but not to erythropoietin (Epo)

British Journal of Haematology

Volumn 98, Issue 3, 1997, Pages 549-559

  Bonsi, Laura ^a   Grossi, Alberto ^b   Strippoli, Pierluigi ^a   Tumietto, Fabio ^a   Tonelli, Roberto ^a   Vannucchi, Alessandro M ^b   Ronchi, Antonella ^c   Ottolenghi, Sergio ^c   Visconti, Giovannella ^b   Avanzi, Gian Carlo ^d   Pegoraro, Luigi ^d   Bagnara, Gian Paolo ^a,e  

^a UNIVERSITY OF BOLOGNA   (Italy)

^b UNIVERSITY OF FLORENCE   (Italy)

^c UNIVERSITY OF MILAN   (Italy)

^d UNIVERSITY OF TURIN   (Italy)

^e Institute of Histology   (Italy)

Author keywords

Cell line; Erythropoietin; Globin chain synthesis; MGDE megakaryocyte

Indexed keywords

ERYTHROPOIETIN; ERYTHROPOIETIN RECEPTOR; GLOBIN; MEGAKARYOCYTE GROWTH AND DEVELOPMENT FACTOR; MESSENGER RNA; THROMBOPOIETIN;

ACUTE GRANULOCYTIC LEUKEMIA; ARTICLE; CELL PROLIFERATION; CYTOGENETICS; ERYTHROID CELL; ERYTHROPOIESIS; GLOBIN SYNTHESIS; HORMONE ACTION; HUMAN; HUMAN CELL; IMMUNOPHENOTYPING; MEGAKARYOCYTE; MEGAKARYOPOIESIS; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; STEM CELL;

EID: 9844253336     PISSN: 00071048     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2141.1997.2793096.x     Document Type: Article

References (35)

1. Avanzi, G.C., Brizzi, M.F., Giannotti, J., Ciarletta, A., Yang, Y.C., Pegoraro, L. & Clark, S.C. (1990) M-07e human leukaemic factor-dependent cell line provides a rapid and sensitive bioassay for the human cytokines GM-CSF and IL3. Journal of Cellular Physiology, 145, 458-464.
2. Bartley, T.D., Bogenberger, J., Hunt, P., Li, Y.-S., Lu, H.S., Martin, F., Chang, M.-S., Samal, B., Nichol, J.L., Swift, S., Johnson, M.J., Hsu, R.-Y., Parker, V.P., Suggs, S., Skrine, J.D., Merewether, L.A., Clogston, C., Hsu, E., Hokom, M.M., Hornkohl, A., Choi, E., Pangelinan, M., Sun, Y., Mar, V., McNinch, J., Simonet, L., Jacobsen, F., Xie, C., Shutter, J., Chute, H., Basu, R., Selander, L., Trollinger, D., Sieu, L., Padilla, D., Trail, G., Elliot, G., Izumi, R., Covey, T., Crouse, J., Garcia, A., Xu, W., del Castillo, J., Biron, J., Cole, S., Hu, M.C.-T., Pacific, R., Ponting, I., Saris, C., Wen, D., Yung, Y.P., Lin, H. & Bosselman, R.A. (1994) Identification and cloning of a megakaryocyte growth and development factor that is a ligand for the cytokine receptor Mpl. Cell, 77, 1117-1124.
3. Chomczynski, P. & Sacchi, N. (1987) Single-step-method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry, 162, 156-159.
4. Church, G.M. & Gilbert, W. (1984) Genomic sequencing. Proceedings of the National Academy of Sciences of the United States of America, 81, 1991-1995.
5. Columbyova, L., Loda, M. & Scadden, D.T. (1995) Thrombopoietin receptor expression in human cancer cell line and primary tissues. Cancer Research, 55, 3509-3512.
6. de Sauvage, F.J., Hass, P.E., Spencer, S.D., Malloy, B.E., Gurey, A.L., Spencer, S.A., Darbonne, W.C., Henzel, W.J., Wong, S.C., Kuang, W.-J., Oles, K.J., Hultgren, B., Solberg, L.A., Jr, Goeddel, D.V. & Eaton, D.L. (1994) Stimulation of megakaryocytopoiesis and thrombopoiesis by the c-Mpl ligand. Nature, 369, 533-538.
7. 3H-serotonin by megakaryocytes and their physiologic and morphological response to stimuli for the platelet release reaction. Laboratory Investigation, 36, 321-328.
8. Fraser, P., Hurst, J., Collis, P. & Grosveld, F. (1990) DNase I hypersensitive sites 1, 2 and 3 of the human β-globin dominant control region direct position-independent expression. Nucleic Acids Research, 18, 3503-3508.
9. Fugman, D.A., Witte, D.P., Jones, C.L.A., Aronow, B.J. & Lieberman, M.A. (1990) In vitro establishment and characterization of a human megakaryoblastic cell line. Blood, 75, 1252-1261.
10. Hoffman, R. (1989) Regulation of megakaryocytopoiesis. Blood, 74, 1196-1212.
11. Iscove, N.N., Senn, S., Till, J.E. & McCulloch, E.A. (1971) Colony formation by normal and leukemic human marrow cells in culture: effect of conditioned medium from normal leukocytes. Blood, 37, 1-5.
12. Itano, M., Tsuchiya, S., Minegishi, N., Fujie, H., Minigishi, M., Morita, S., Yambe, T., Ohashi, Y., Masuda, T., Koike, T. & Konno, T. (1995) Establishment and characterization of a novel human immature megakaryoblastic leukemia cell line, M-MOK, dependent on fibroblast for its viability. Experimental Hematology, 23, 1301-1309.
13. Kaushansky, K., Lok, S., Holly, R.D., Broudy, V.C., Lin, N., Bailey, M.C., Forstrom, J.W., Buddle, M.M., Oort, P.J., Hagen, F.S., Roth, G.J., Papayannopoulou, T. & Foster, D.C. (1994) Promotion of megakaryocyte progenitor expansion and differentiation by the c-mpl ligand thrombopoietin. Nature, 369, 568-571.
14. Kitamura, T., Tange, T., Terasawa, T., Chiba, S., Kuwaki, T., Miyagawa, K., Piao, Y.F., Miyazono, K., Urabe, A. & Takaku, F. (1989) Establishment and characterization of a unique human line that proliferates dependently on GM-CSF, IL3 or erythropoietin. Journal of Cellular Physiology, 140, 323-334.
15. Kobayashi, M., Laver, J.H., Kato, T., Miyazaki, H. & Ogawa, M. (1995) Recombinant human thrombopoietin (Mpl ligand) enhances proliferation of erythroid progenitors. Blood, 86, 2494-2499.
16. Komatsu, N., Nakauchi, H., Miwa, A., Ishihara, T., Eguchi, M., Moroi, M., Okada, M., Sato, Y., Wada, H., Yawata, Y., Suda, T. & Miura, Y. (1991) Establishment and characterization of a human leukaemic cell line with megakaryocytic features: dependency on granulocyte-macrophage colony-stimulating factor, interleukin 3, or erythropoietin for growth and survival. Cancer Research, 51, 341-348.
17. Levtin, N.L., Linch, D.C., Beardsley, G.P., McIntyre, K.W. & Nathan, D. (1984) Augmentation of fetal hemoglobin production in anemic monkeys by hydroxyurea. New England Journal of Medicine, 310, 869-873.
18. Martin, P. & Papayannopoulou, T. (1982) HEL cells: a new human erythroleukemia cell line with spontaneous and induced globin expression. Science, 216, 1233-1235.
19. Matsumura, I., Kanakura, Y., Kato, T., Ikeda, H., Ishikaw, J., Horikawa, Y., Hashimoto, K., Moriyama, Y. Tsujimura, T., Nishiura, T., Miyazaki, H. & Matsuzawa, Y. (1995) Growth response of acute myeloblastic leukemia cells to recombinant human thrombopoietin. Blood, 86, 703-709.
20. Methia, N., Louache, F., Vainchenker, W. & Wendling, F. (1993) Oligodeoxynucleotides antisense to the proto-oncogene c-mpl specifically inhibit in vitro megakaryocytopoiesis. Blood, 82, 1395-1401.
21. Mouthon, M.A., Freud, M., Titeux, M., Katz, A., Guichard, J., Breton-Gorius, J. & Vainchenker, W. (1994) Growth and differentiation of the human megakaryoblastic cell line (ELF-153): a model for early stages of megakaryocytopoiesis. Blood, 84, 1085-1097.
22. Ogura, M., Morishima, Y., Ohno, R., Kato, Y., Hirabayashi, N., Nagura, H. & Saito, H. (1985) Establishment of a novel human megakaryoblastic leukemia cell line, MEG-01, with positive Philadelphia chromosome. Blood, 66, 1384-1392.
23. Papayannopoulou, T., Brice, N., Farrer, D. & Kaushansky, K. (1996) Insights into the cellular mechanisms of erythropoietin-thrombopoietin synergy. Experimental Hematology, 24, 660-669.
24. Perrine, S.P., Ginder, G.D., Faller, D.V., Dover, G.H., Ikuta, T., Witkowska, E., Shi-Ping, C., Vichinsky, E.P. & Olivieri, N.F. (1993) A short-term trial of butyrate to stimulate fetal-globin gene expression in the β-globin disorders. New England Journal of Medicine, 328, 81-86.
25. Rossi, E.C. (1972) The effect of albumin upon the loss of enzymes from washed platelets. Journal of Laboratory and Clinical Medicine, 79, 240-246.
26. Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular Cloning. Cold Spring Harbor Laboratory, New York.
27. Seabright, M.A. (1971) A rapid banding technique for human chromosomes. Lancet, ii, 971-972.
28. Takeuchi, K., Ogura, M., Saito, H., Satoh, M. & Takeuchi, M. (1991) Production of platelet-like particles by a human megakaryoblastic leukemia cell line (MEG-01). Experimental Cell Research. 1993, 223-226.
29. Tsuyuoka, R., Takahashi, T., Suzuki, A., Sasaki, Y., Kishiko, N., Fukumoto, M., Ohmori, K., Ohno, Y. & Nakao, K. (1995) A newly established megakaryoblastic/erythroid cell line that differentiates to red cells in the presence of erythropoietin and produces platelet-like particles. Stem Cells, 13, 54-64.
30. Vannucchi, A.M., Grossi, A., Paoletti, E. Rafanelli, D., Bacci, P. & Rossi Ferrini, P. (1992) Constitutive expression of a megakaryocytic functional property by murine erythroleukemia (Friend) cells: the incorporation of serotonin. Experimental Hematology, 20, 1296-1301.
31. Vannucchi, A.M., Grossi, A., Rafanelli, D., Vannucchi, L. & Rossi Ferrini, P. (1988) Serotonin incorporation as a marker of murine megakaryocyte proliferation in liquid cultures. Experimental Hematology, 16, 916-921.
32. Vannucchi, A.M., Paoletti, F. & Grossi, A. (1994) A common megakaryocytic and erythrocytic precursor in murine erythroleukemia (Friend) cells. Experimental Hematology, 22, 110-111.
33. Vigon, I., Florindo, C., Fichelson, S., Guenet, J., Mattei, M., Soury, M., Cosman, D. & Gisselbrecht, S. (1993) Characterization of the murine MPL proto-oncogene, a member of the hemopoietic cytokine receptor family: molecular cloning, chromosomal location and evidence for a function in cell growth. Oncogene, 8, 2607-2615.
34. Vigon, I., Mornon, J.P., Cocault, L., Mitjavila, M.T., Tamborin, P., Gisselbrecht, S. & Souyri, M. (1992) Molecular cloning and characterization of MPL, the human homolog of the v-mpl oncogene: identification of a member of the hematopoietic growth factor receptor superfamily. Proceedings of the National Academy of Sciences of the United States of America, 89, 5640-5644.
35. Wendling, F., Maraskovsky, E., Debili, N., Florindo, C., Teepe, M., Titeux, M., Methia, N., Breton-Gorius, J., Cosman, D. & Vainchenker, W. (1994) c-Mpl ligand is a humoral regulator of megakaryocytopoiesis. Nature, 369, 571-574.