Increased yield of PCR product from degenerate primers with nondegenerate, nonhomologous 5′ tails

BioTechniques

Volumn 38, Issue 1, 2005, Pages 34-38

  Regier, Jerome C ^a   Shi, Diane ^a  

^a UNIVERSITY OF MARYLAND BIOTECHNOLOGY INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PRIMER RNA;

5' UNTRANSLATED REGION; AMINO ACID SEQUENCE; ARTHROPOD; ARTICLE; CONTROLLED STUDY; CRUSTACEA; ELECTROPHORESIS; NONHUMAN; REVERSE TRANSCRIPTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY;

ARTHROPODA; CRUSTACEA;

EID: 9744222368     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/05381BM02     Document Type: Article

References (5)

1. Regier, J.C. and J.W. Shultz. 2001. Elongation factor-2: a useful gene for arthropod phylogenetics. Mol. Phylogenet. Evol. 20:136-148.
2. Cho, S., A. Mitchell, J.C. Regier, C. Mitter, R.W. Poole, T.P. Friedlander, and S. Zhao. 1995. A highly conserved nuclear gene for low-level phylogenetics: elongation factor-1α recovers morphology-based tree for heliothine moths. Mol. Biol. Evol. 12:650-656.
3. Rose, T.M., E.R. Schultz, J.G. Henikoff, S. Peitrokovski, C.M. McCallum, and S. Henikoff. 1998. Consensus-degenerate hybrid oligonucleotide primers for amplification of distantly related sequences. Nucleic Acids Res. 26:1628-1635.
4. Rose, T.M., J.G. Henikoff, and S. Henikoff. 2003. CODEHOP (COnsensus-DEgenerate Hybrid Oliognucleotide Primer) PCR primer design. Nucleic Acids Res. 31:3763-3766.
5. Don, R.H., P.T. Cox, B.J. Wainwright, K. Baker, and J.S. Mattick. 1991. 'Touchdown' PCR to circumvent spurious priming during gene ampification. Nucleic Acids Res. 19:4008.