Preparation and primary application of monoclonal antibodies against a novel ribosome-inactivating protein Moschatin from pumpkin seeds

Acta Biochimica et Biophysica Sinica

Volumn 36, Issue 2, 2004, Pages 105-110

  Xia, Heng Chuan ^a   Hu, Wei Guo ^a   Yang, Xin Xiu ^a   Li, Feng ^a   Zhang, Zu Chuan ^a  

^a INSTITUTE OF BIOCHEMISTRY AND CELL BIOLOGY   (China)

Author keywords

Affinity chromatography; Monoclonal antibody; Moschatin; Western blot

Indexed keywords

IMMUNOGLOBULIN G; IMMUNOGLOBULIN M; MONOCLONAL ANTIBODY; MOSCHATIN; RIBOSOME INACTIVATING PROTEIN; SEPHAROSE; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ANTIBODY AFFINITY; ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; HYBRIDOMA; IMMUNOGLOBULIN PRODUCTION; MOUSE; NONHUMAN; PROTEIN ANALYSIS; PROTEIN PURIFICATION; PUMPKIN; WESTERN BLOTTING;

ANIMALIA;

EID: 9644279223     PISSN: 05829879     EISSN: None     Source Type: Journal    
DOI: 10.1093/abbs/36.2.105     Document Type: Article

References (29)

1. Endo Y, Tsurugi K. RNA N-glycosidase activity of ricin A-chain. Mechanism of action of the toxic lectin ricin on eukaryotic ribosomes. J Biol Chem, 1987, 262(17): 8128-8130
2. Zhang JS, Liu WY. The mechanism of action of trichosanthin on eukaryotic ribosomes - RNA N-glycosidase activity of the cytotoxin. Nucleic Acids Res, 1992, 20(10): 1271-1275
3. Stirpe F, Barbieri L. Ribosome-inactivating proteins up to date. FEBS Lett, 1986, 195(1-2): 1-8
4. Lambert JM, Blattler WA, McIntyre GD, Goldmacher VS, Scott CF Jr. Immunotoxins containing single-chain ribosome-inactivating proteins. Cancer Treat Res, 1988, 37: 175-209
5. Park SW, Stevens NM, Vivanco JM. Enzymatic specificity of three ribosome-inactivating proteins against fungal ribosomes, and correlation with antifungal activity. Planta, 2002, 216(2): 227-234
6. Ng TB, Parkash A. Hispin, a novel ribosome inactivating protein with antifungal activity from hairy melon seeds. Protein Expr Purif, 2002, 26(2): 211-217
7. Lam SK, Ng TB. Hypsin, a novel thermostable ribosome-inactivating protein with antifungal and antiproliferative activities from fruiting bodies of the edible mushroom Hypsizigus marmoreus. Biochem Biophys Res Commun, 2001, 285(4): 1071-1075
8. Nielsen K, Payne GA, Boston RS. Maize ribosome-inactivating protein inhibits normal development of Aspergillus nidulans and Aspergillus flavus. Mol Plant Microbe Interact, 2001, 14(2): 164-172
9. Ng TB, Chan WY, Yeung HW. Proteins with abortifacient, ribosome inactivating, immunomodulatory, antitumor and anti-AIDS activities from Cucurbitaceae plants. Gen Pharmacol, 1992, 23(4): 579-590
10. Ng TB, Liu WK, Sze SF, Yeung HW. Action of alpha-momorcharin, a ribosome inactivating protein, on cultured tumor cell lines Gen Pharmacol, 1994, 25(1): 75-77
11. Kong M, Ke YB, Zhou MY, Ke XY, Lu B, Nie HL. Study on trichosanthin induced apoptosis of leukemia K562 cells. Shi Yan Sheng Wu Xue Bao, 1998, 31(3): 233-243
12. Bolognesi A, Tazzari PL, Olivieri F, Polito L, Falini B, Stirpe F. Induction of apoptosis by ribosome-inactivating proteins and related immunotoxins. Int J Cancer, 1996, 68(3): 349-355
13. Olmo N, Turnay J, de Buitrago GG, de Silanes IL, Gavilanes JG, Lizarbe MA. Cytotoxic mechanism of the ribotoxin alpha-sarcin. Induction of cell death via apoptosis. Eur J Biochem, 2001, 268(7): 2113-2123
14. Benigni F, Canevari S, Gadina M, Adobati E, Ferreri AJM, di Celle EF, Comolli R et al. Preclinical evaluation of the ribosome-inactivating proteins PAP-1, PAP-S and RTA in mice. Int J Immunopharmacol, 1995, 17(10): 829-839
15. Zhao J, Ben LH, Wu YL, Hu W, Ling K, Xin SM, Nie HL et al. Anti-HIV agent trichosanthin enhances the capabilities of chemokines to stimulate chemotaxis and G protein activation, and this is mediated through interaction of trichosanthin and chemokine receptors. J Exp Med, 1999, 190(1): 101-111
16. Wachinger M, Samtleben R, Gerhauser C, Wagner H, Erfle V. Bryodin, a single-chain ribosome-inactivating protein, selectively inhibits the growth of HIV-1-infected cells and reduces HIV-1 production. Res Exp Med (Berl), 1993, 193(1): 1-12
17. Au TK, Collins RA, Lam TL, Ng TB, Fong WP, Wan DC. The plant ribosome inactivating proteins luffin and saporin are potent inhibitors of HIV-1 integrase. FEBS Lett, 2000, 471(2-3): 169-172
18. Peumans WJ, Hao Q, van Damme EJ. Ribosome-inactivating proteins from plants: More than RNA N-glycosidases? FASEB J, 2001, 15(9): 1493-1506
19. Lamb CJ, Ryals JA, Ward ER, Dixon RA. Emerging strategies for enhancing crop resistance to microbial pathogens. Biotechnology (NY), 1992, 10(11): 1436-1445
20. Girbes T, Ferreras JM, Iglesias R, Citores L, De Torre C, Carbajales ML, Jimenez P et al. Recent advances in the uses and applications of ribosome-inactivating proteins from plants. Cell Mol Biol (Noisy-le-grand), 1996, 42(4): 461-471
21. Nielsen K, Boston RS. Ribosome-inactivating proteins: A plant perspective. Annu Rev Plant Physiol Plant Mol Biol, 2001, 52: 785-816
22. Xia HC, Li F, Li Z, Zhang ZC. Purification and characterization of Moschatin, a novel type I ribosome-inactivating protein from the mature seeds of pumpkin (Cucurbita moschata), and preparation of its immunotoxin against human melanoma cells. Cell Res, 2003, 13(5): 369-374
23. Gao WD, Cao HT, Ji RH, Zhang ZC. Isolation and characterization of protein-biosynthesis inhibiting proteins from seeds of Luffa cylindrica. Acta Biochim Biophys Sin, 1994; 26(3): 289-295
24. Harlow E, Lane D. Antibodies: A Laboratory Manual. New York: Cold Spring Harbor Laboratory Press, 1988
25. Douillard JY, Hoffman T. Enzyme-linked immunosorbent assay for screening monoclonal antibody production using enzyme-labeled second antibody. Methods Enzymol, 1983, 92: 168-174
26. Yan M, Yang XX, Zhang RP, Ping BF, Li Z, Zhang ZC. Preparation and use of a monoclonal antibody against Luffin b. Acta Biochim Biophys Sin, 2001, 33(1): 93-98
27. Beatty JD, Beatty BG, Vlahos WG. Measurement of monoclonal antibody affinity by non-competitive enzyme immunoassay. J Immunol Methods, 1987, 100(1-2): 173-179
28. Stirpe F, Barbieri L, Battelli MG, Soria M, Lappi DA. Ribosome-inactivating proteins from plants: present status and future prospects. Biotechnology (NY), 1992, 10(4): 405-412
29. Singh RC, Singh V. Current status of ribosome inactivating proteins. Indian J Biochem Biophys, 2000, 37(1): 1-5