Activity-dependent fluorescent labeling of bacterial cells expressing the TOL pathway

Journal of Microbiological Methods

Volumn 60, Issue 1, 2005, Pages 41-46

  Clingenpeel, Scott R ^a   Keener, William K ^b   Keller, Caron R ^c   De Jesus, Karl ^c   Howard, M Hope ^d   Watwood, Mary E ^d  

^a Nucl Eng Idaho State University   (United States)

^b IDAHO NATIONAL LABORATORY   (United States)

^c IDAHO STATE UNIVERSITY   (United States)

^d NORTHERN ARIZONA UNIVERSITY   (United States)

Author keywords

3 Ethynylbenzoate; Bioremediation; Chromogenic; Fluorogenic; Toluene

Indexed keywords

3 ETHYNYLBENZOATE; BENZOIC ACID; BENZOIC ACID DERIVATIVE; CATECHOL; FLUORESCENT DYE; TOLUENE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL CELL; BACTERIUM IDENTIFICATION; CONTROLLED STUDY; ENZYME ANALYSIS; MICROBIAL ACTIVITY; MOLECULAR PROBE; NONHUMAN; PRIORITY JOURNAL;

BENZOATES; FLUORESCENT DYES; MICROSCOPY, FLUORESCENCE; OXYGENASES; PSEUDOMONAS PUTIDA; TOLUENE;

BACTERIA (MICROORGANISMS);

EID: 9644257120     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2004.08.018     Document Type: Article

References (21)

1. M.A. Abril, C. Michan, K.N. Timmis, and J.L. Ramos Regulator and enzyme specificities of the TOL plasmid-encoded upper pathway for degradation of aromatic hydrocarbons and expansion of the substrate range of the pathway J. Bacteriol. 171 1989 6782 6790
2. W.B. Austin, N. Bilow, W.J. Kelleghan, and K.S.Y. Lau Facile synthesis of ethynylated benzoic acid derivatives and aromatic compounds via ethynyltrimethylsilane J. Org. Chem. 46 1981 2280 2286
3. K.H. Engesser, R.B. Cain, and H.J. Knackmuss Bacterial metabolism of side chain fluorinated aromatics: cometabolism of 3-trifluoromethyl(TFM)-benzoate by Pseudomonas putida (arvilla) mt-2 and Rhodococcus rubropertinctus N657 Arch. Microbiol. 149 1988 188 197
4. D.T. Gibson Assay of enzymes of aromatic metabolism J.R. Norris D.W. Ribbons Methods in Microbiology vol. 6A 1972 Academic Press, Inc. New York 463 478
5. S. Harayama, M. Rekik, M. Wubbolts, K. Rose, R.A. Leppik, and K.N. Timmis Characterization of five genes in the upper-pathway operon of TOL plasmid pWW0 from Pseudomonas putida and identification of the gene products J. Bacteriol. 171 1989 5048 5055
6. W.H. Jeffrey, S.M. Cuskey, P.J. Chapman, S. Resnick, and R.H. Olsen Characterization of Pseudomonas putida mutants unable to catabolize benzoate: cloning and characterization of Pseudomonas genes involved in benzoate catabolism and isolation of a chromosomal DNA fragment able to substitute for xylS in activation of the TOL lower-pathway promoter J. Bacteriol. 174 1992 4986 4996
7. G.R. Johnson, and R.H. Olsen Multiple pathways for toluene degradation in Burkholderia sp. strain JS150 Appl. Environ. Microbiol. 63 1997 4047 4052
8. M.E. Kauffman, W.K. Keener, S.R. Clingenpeel, M.E. Watwood, D.W. Reed, Y. Fujita, and R.M. Lehman Use of 3-hydroxyphenylacetylene for activity-dependent, fluorescent labeling of bacteria that degrade toluene via 3-methylcatechol J. Microbiol. Methods 55 2003 801 805
9. W.K. Keener, M.E. Watwood, and W.A. Apel Activity-dependent fluorescent labeling of bacteria that degrade toluene via toluene 2,3-dioxygenase Appl. Microbiol. Biotechnol. 49 1998 455 462
10. W.K. Keener, M.E. Watwood, K.D. Schaller, M.R. Walton, J.K. Partin, W.A. Smith, and S.R. Clingenpeel Use of selective inhibitors and chromogenic substrates to differentiate bacteria based on toluene oxygenase activity J. Microbiol. Methods 46 2001 171 185
11. H. Keil, S. Keil, R.W. Pickup, and P.A. Williams Evolutionary conservation of genes coding for meta pathway enzymes within TOL plasmids pWW0 and pWW53 J. Bacteriol. 164 1985 887 895
12. R.H. Olsen, J.J. Kukor, and B. Kaphammer A novel toluene-3-monooxygenase pathway cloned from Pseudomonas pickettii PKO1 J. Bacteriol. 176 1994 3749 3756
13. G. Paller, R.K. Hommel, and H.P. Kleber Phenol degradation by Acinetobacter calcoaceticus NCIB 8250 J. Basic Microbiol. 35 1995 325 335
14. M.S. Shields, S.O. Montgomery, P.J. Chapman, S.M. Cuskey, and P.H. Pritchard Novel pathway of toluene catabolism in the trichloroethylene-degrading bacterium G4 Appl. Environ. Microbiol. 55 1989 1624 1629
15. R.L. Shriner, R.C. Fuson, D.Y. Curtin, and T.C. Morrill The Systematic Identification of Organic Compounds 6th Edn. 1980 John Wiley New York 167
16. L.P. Wackett, and D.T. Gibson Degradation of trichloroethylene by toluene dioxygenase in whole-cell studies with Pseudomonas putida F1 Appl. Environ. Microbiol. 54 1988 1703 1708
17. G.M. Whited, W.R. McCombie, L.D. Kwart, and D.T. Gibson Identification of cis-diols as intermediates in the oxidation of aromatic acids by a strain of Pseudomonas putida that contains a TOL plasmid J. Bacteriol. 166 1986 1028 1039
18. G.M. Whited, and D.T. Gibson Separation and partial characterization of the enzymes of the toluene-4-monooxygenase catabolic pathway in Pseudomonas mendoccina KR1 J. Bacteriol. 173 1991 3017 3020
19. P.A. Williams, and M.J. Worsey Ubiquity of plasmids in coding for toluene and xylene metabolism in soil bacteria: evidence for the existence for new TOL plasmids J. Bacteriol. 125 1976 818 828
20. M.J. Worsey, and P.A. Williams Metabolism of toluene and xylenes by Pseudomonas putida (arvilla) mt-2: evidence for a new function of the TOL plasmid J. Bacteriol. 124 1975 7 13
21. J. Zeyer, P.R. Lehrbach, and K.N. Timmis Use of cloned genes of Pseudomonas TOL plasmid to effect biotransformation of benzoates to cis-dihydrodiols and catechols by Escherichia coli cells Appl. Environ. Microbiol. 50 1985 1409 1413