Determination of activable proacrosin/acrosin in bovine sperm using an irreversible isocoumarin serine protease inhibitor

Biology of Reproduction

Volumn 55, Issue 3, 1996, Pages 536-542

  Palencia, D D ^a   Garner, D L ^a   Hudig, D ^a   Holcombe, D W ^a   Burner, C A ^a   Redelman, D ^b   Fernandez, G C J ^a   Abuelyaman, A S ^c   Kam, C M ^c   Powers, J C ^c  

^a UNIVERSITY OF NEVADA   (United States)

^b Sierra Cytometry   (United States)

^c GEORGIA INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACROSIN; ISOCOUMARIN DERIVATIVE; PROACROSIN; SERINE PROTEINASE INHIBITOR;

ANIMAL CELL; ARTICLE; CATTLE; CRYOPRESERVATION; FEMALE; FERTILIZATION; NONHUMAN; PRIORITY JOURNAL; SPERM; SPERMATOZOON PENETRATION; ZONA PELLUCIDA;

ANIMALIA; BOS TAURUS; BOVINAE;

EID: 9544246725     PISSN: 00063363     EISSN: None     Source Type: Journal    
DOI: 10.1095/biolreprod55.3.536     Document Type: Article

References (26)

1. Perreault SD, Zirkin BR. Sperm nuclear decondensation in mammals: role of sperm-associated proteinase in vivo relevant to domesticated mammals. J Exp Zool 1982; 224:253-257.
2. De Jonge CJ, Mack SR, Zaneveld LJD. Inhibition of the human sperm acrosome reaction by proteinase inhibitors. Gamete Res 1989; 23: 387-398.
3. Pillai MC, Meizel S. Trypsin inhibitors prevent the progesterone-initiated increase in intracellular calcium required for the human sperm acrosome reaction. J Exp Zool 1991; 258:384-393.
4. Llanos M, Vigil P, Salgado AM, Morales P. Inhibition of the acrosome reaction by trypsin inhibitor and prevention of penetration of spermatozoa through the human zona pellucida. J Reprod Fertil 1993; 97: 173-178.
5. Baba T, Azuma S, Kashiwabara S, Toyoda Y. Sperm from mice carrying a targeted mutation of the acrosin gene can penetrate the oocyte zona pellucida and effect fertilization. J Biol Chem 1994; 269:31845-31849.
6. Tummon IS, Yuzpe AA, Daniel SA, Deutsch A. Total acrosin activity correlates with fertility potential after fertilization in vitro. Fertil Steril 1991; 56:933-938.
7. Francavilla S, Palermo G, Gabriele A, Cordeschi G, Poccia G. Sperm acrosin activity and fluorescence microscopic assessment of proacrosin/acrosin in ejaculates of infertile and fertile men. Fertil Steril 1992; 57:1311-1316.
8. De Jonge CJ, Tarchala SM, Rawlins RG, Binor Z, Radwariska E. Acrosin activity in human spermatozoa in relation to semen quality and in-vitro fertilization. Hum Reprod 1993; 8:253-257.
9. Koukoulis GN, Vantman D, Dennison L, Banks SM, Sherins RJ. Low acrosin activity in a subgroup of men with idiopathic infertility does not correlate with sperm density, percent motility, curvilinear velocity, or linearity. Fertil Steril 1989; 52:120-127.
10. Tesarik J, Drahorad J, Testart J, Mendoza C. Acrosin activation follows its surface exposure and precedes membrane fusion in human sperm acrosome reaction. Development 1990; 110:391-400.
11. Senn A, Germond M, De Grandi P. Immunofluorescence study of actin, acrosin, dynein, tubulin and hyaluronidase and their impact on in vitro fertilization. Hum Reprod 1992; 7:841-849.
12. Kennedy WP, Kaminski JM, Van der Yen HH, Jeyendran RS, Reid DS, Blackwell J, Bielfeld P, Zaneveld LJ. A simple, clinical assay to evaluate the acrosin activity of human spermatozoa. J Androl 1989; 10:221-231.
13. Muller-Esterl W, Fritz M. Sperm acrosin. Methods Enzymol 1981; 80: 621-632.
14. NagDas SK. Bovine epididymal sperm proacrosin-acrosin system: quantification and partial characterization. Andrologia 1992; 24:171-178.
15. Kam C-M, Abuelyaman AS, Li Z, Hudig D, Powers JC. Biotinylated isocoumarins, new inhibitors and reagents for detection, localization, and isolation of serine proteases. Bioconjugate Chem 1993; 4:560-567.
16. Powers JC, Kam CA, Narasimhan L, Oleksyszyn J, Hemandez MA, Ueda T. Mechanism-based isocoumarin inhibitors for serine proteases: use of active site structure and substrate specificity in inhibitor design. J Cell Biochem 1989; 39:33-46.
17. Almquist JO, Allen CH, Branas RJ. Effect on fertility of large number of straws on bovine spermatozoa in a mechanical freezer. J Anim Sci 1982; 55:232-235.
18. Johnson MS, Senger PL, Allen CH, Hancock DD, Alexander BM, Sasser RG. Fertility of bull semen packaged in .25 and .5 milliliter French straws. J Animal Sci 1995; 73:1914-1919.
19. Lessley BA, Garner DL. Isolation of motile spermatozoa by density gradient centrifugation. Gamete Res 1983; 7:49-61.
20. Zahler WL, Polakoski KL. Benzamidine as an inhibitor of proacrosin activation in bull sperm. Biochem Biophys Acta 1977; 480:461-468.
21. Overton WR. Modified histogram subtraction technique for analysis of flow cytometry data. Cytometry 1988; 9:619-626.
22. Havermehl PE. Suggested corrections for Overton's Pascal procedure. Cytometry 1989; 10:492-493.
23. Garner DL, Easton MP, Munson ME, Doane MA. Immunofluorescent localization of bovine acrosin. J Exp Zool 1975; 191:127-131.
24. Holt WV, North RD. Effects of temperature and restoration of osmotic equilibrium during thawing on the induction of plasma membrane damage in cryopreserved ram spermatozoa. Biol Reprod 1994; 51: 414-424.
25. Harper JW, Hemmi K, Powers JC. Reaction of serine proteases with substituted isocoumarins: discovery of 3.4-dichloroisocoumarin, a new general mechanism based serine protease inhibitor. Biochemistry 1985; 24:1831-1841.
26. Amann RP, Hammerstedt RH. In vitro evaluation of sperm quality: an opinion. J Androl 1993; 14:397-406.