Conformational flexibility and crystallization of tandemly linked type III modules of human fibronectin

Protein Science

Volumn 5, Issue 9, 1996, Pages 1934-1938

  Lombardo, A ^a   Wang, Y ^a   Ni, C Z ^a   Dai, X ^a   Dickinson, C D ^d   Kodandapani, R ^a   Chiang, S ^a   White, C A ^a   Pio, F ^a   Xuong, N H ^b   Hamlin, R C ^c   Ruoslahti, E ^a   Ely, K R ^a  

^a BURNHAM INSTITUTE   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c Area Detector Systems Corporation   (United States)

^d SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

cell adhesion; conformational flexibility; crystallization; fibronectin; integrins

Indexed keywords

FIBRONECTIN; INTEGRIN;

ARTICLE; CELL ADHESION; CONFORMATIONAL TRANSITION; CRYSTALLIZATION; EXTRACELLULAR MATRIX; GENE REARRANGEMENT; NONHUMAN; PHAGOCYTOSIS; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN CONFORMATION; PROTEIN DOMAIN; TANDEM REPEAT; WOUND HEALING;

EID: 9544219686     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1002/pro.5560050922     Document Type: Article

References (38)

1. Aguirre KM, McCormick RJ, Schwarzbauer JE. 1994. Fibronectin self-association is mediated by complementary sites within the amino-terminal one-third of the molecule. J Biol Chem 269:27863-27868.
2. Bowditch RD, Hariharan M, Tominna EF, Smith JW, Yamada KM, Getzoff ED, Ginsberg MH. 1994. Identification of a novel integral binding site in fibronectin. J Biol Chem 269:10856-10863.
3. Bork P, Doolittle RF. 1992. Proposed acquisition of an animal protein domain by bacteria. Proc Natl Acad Sci USA 89:8990-8994.
4. DeVos AM, Ultsch M, Kossiakoff AA. 1992. Human growth hormone and extracellular domain of its receptor Crystal structure of the complex. Science 255:306-312.
5. Dickinson CD, Veerapandian B, Dai XP, Hamlin RC, Xuong NH, Ruoslahti E, Ely KR 1994. Crystal structure of the tenth type III cell adhesion module of human fibronectin. J Mol Biol 236:1079-1092.
6. Doolittle RF, Bork P. 1993. Evolutionary mobile modules in proteins. Sci Am 269:50-56.
7. Ely KR, Kunicki TJ, Kodandapani R. 1995. Common molecular scaffold for two unrelated RGD molecules. Protein Eng 8:823-827.
8. Engel J, Odermatt E, Engels A, Madri JA, Furthmayr H, Rohdeh H, Timpl H, Shapes R. 1981. Domain organizations and flexibility of laminin and fibronectin, two multifunctional proteins of the extracellular matrix. J Mol Biol 150:97-120.
9. Erickson HP, Carrell N, Mcdonagh J. 1981. Fibronectin molecule visualized in electron microscopy: A long thin flexible strand. J Cell Biol 91:673-678.
10. Ferre-D'Amare AR, Burley SK. 1994. Use of dynamic light scattering to assess crystallizability of macromolecules and macromolecular assemblies Structure 2:357-359.
11. Guddat LW, Herron JN, Edmundson AB. 1993. Three-dimensional structure of a human immunoglobulin with a hinge deletion. Proc Natl Acad Sci USA 90:4271-4275.
12. Huber AH, Wang YME, Bierber AJ, Bjorkman PJ. 1994. Crystal structure of tandem type III fibronectin domains from Drosophila neuroglian at 2 Å. Neuron 12:717-731.
13. Hynes RO. 1990. Fibronectins. New York: Springer-Verlag.
14. Jancarik J, Kim SH. 1991. Sparse matrix sampling - A screening method for crystallization of proteins. J Appl Crystallogr 24:409-411
15. Jones EY, Davis SJ, Williams AF, Harlos K, Stuart DI. 1992. Crystal structure at 2.8 Å resolution of a soluble form of the cell adhesion molecule CD2. Nature 360:232-239.
16. Kornblihtt AR, Umezawa K, Vibe-Pederson K, Baralle FE. 1985. Primary structure of human fibronectin: Differential splicing may generate at least 10 polypeptides from a single gene. EMBO J 4:1755-1759.
17. Leahy DJ, Aukhil I, Erickson HP. 1996. 2.0 Å crystal structure of a four-domain segment of human fibronectin encompassing the RGD loop and synergy region. Cell 84:155-164.
18. Leahy DJ, Hendrickson WA, Aukhil I, Erickson HP. 1992. Structure of a fibronectin type III domain from tenascin phased by MAD analysis of the selenomethionyl protein. Science 258:987-991.
19. LePage Y. 1982. The derivation of the axes of the conventional unit cell from the dimensions of the Buerger-reduced cell. J Appl Crystallogr 15:255-259.
20. Litvinovich SV, Ingham KC. 1995. Interactions between type III domains in the 110 kDa cell-binding fragment of fibronectin. J Mol Biol 248:611-626.
21. Main AL, Harvey TS, Baron M, Boyd J, Campbell ID 1992. The three-dimensional structure of the tenth type III module of fibronectin: An insight into RGD-mediated interactions. Cell 71:671-678.
22. McKeown-Longo PJ, Mosher DF. 1985. Interaction of the 70,000-mol-wt amino-terminal fragment of fibronectin with the matrix-assembly receptor of fibroblasts. J Cell Biol 100:364-374.
23. McPherson A. 1982. Preparation and analysis of protein crystals. New York. Wiley-Interscience.
24. Morla A, Ruoslahti E. 1992. A fibronectin self-assembly site involved in fibronectin matrix assembly: Reconstruction in a synthetic peptide. J Cell Biol 118:421-429.
25. Morla A, Zhang Z, Ruoslahti E. 1994. Superfibronectin is a functionally distinct form of fibronectin. Nature 367:193-196.
26. Mosher DF, Somle J, Wu C, McDonald JA. 1992. Assembly of extracellular matrix. Curr Opin Cell Biol 4:810-818.
27. Obara M, Kang MS, Yamada KM. 1988. Site directed-mutagenesis of the cell-binding domain of human fibronectin: Separable, synergistic sites mediate adhesive function. Cell 53:649-657.
28. Otwinowski Z. 1993. Oscillation data reduction program. In: Sawyer L, Isaacs N, Bailey S, eds. Data collection and processing. Warrington, England: Science and Engineering Research Council/Daresbury Laboratory, pp 56-62.
29. Patthy L. 1990. Homology of a domain of the growth hormone prolactin receptor family with type III module of fibronectin. Cell 61:13-14
30. Pechik I, Nachman I, Ingham K, Gilliland GL. 1993. Crystallization and preliminary X-ray diffraction data of two heparin-binding fragments of human fibronectin. Proteins Struct Funct Genet 16:43-47.
31. Peters DM, Mosher DF. 1987. Localization of cell surface sites involved in fibronectin fibrillogenesis. J Cell Biol 104:121-130.
32. Pierschbacher MD, Ruoslahti E. 1984. Cell attachment activity of fibronectin can be duplicated by small synthetic fragments of the molecule. Nature 309:30-33.
33. Quade BJ, McDonald JA. 1988. Fibronectin's amino-terminal matrix assembly site is located within the 29-kDa amino-terminal domain containing five type 1 repeats. J Biol Chem 263:19602-19609.
34. Ruoslahti E. 1988. Fibronectin and its receptors. Annu Rev Biochem 57:375-413.
35. Ryu SE, Truneh A, Sweet RW, Hendrickson WA. 1994 Structures of an HIV and MHC binding fragment from human CD4 as refined in two crystal lattices Structure 2:59-74.
36. Sambrook J, Fritsch EF, Maniatis T. 1989. Molecular cloning: A laboratory manual, 2nd ed. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
37. Schwarzbauer JE. 1991. Identification of the fibronectin sequences required for assembly of a fibrillar matrix. J Cell Biol 113:1463-1473.
38. Vrielink A, Beamer L, Le T, Eisenberg D. 1995. Crystallization of the chaperone protein SecB. Protein Sci 4:1651-1653.