Construction of the plasmid pMEX8-hAK1 and random site-directed mutagenesis of human cytosolic adenylate kinase

Biochemistry and Molecular Biology International

Volumn 38, Issue 2, 1996, Pages 373-381

  Ayabe, Takanori ^a   Takenaka, Hitoshi ^a   Takenaka, Osamu ^b   Takenaka, Akiko ^c   Nagahama, Hiroyuki ^a   Maruyama, Hideharu ^a   Yamamoto, Atsushi ^a   Nagata, Masahiko ^a   Koga, Yasunori ^a   Sumida, Michihiro ^d   Hamada, Minoru ^a  

^a UNIVERSITY OF MIYAZAKI   (Japan)

^b KYOTO UNIVERSITY   (Japan)

^c Nagoya Bunri College   (Japan)

^d EHIME UNIVERSITY SCHOOL OF MEDICINE   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ADENYLATE KINASE; LYSINE; SINGLE STRANDED DNA; THREONINE;

ARTICLE; BACTERIOPHAGE; CONTROLLED STUDY; ENZYME ACTIVITY; GENETIC ENGINEERING; HUMAN; NONHUMAN; PLASMID; PROTEIN EXPRESSION; SITE DIRECTED MUTAGENESIS;

ADENYLATE KINASE; AMINO ACID SEQUENCE; BASE SEQUENCE; CYTOSOL; DNA MUTATIONAL ANALYSIS; GENETIC VECTORS; HUMANS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PLASMIDS; SUBSTRATE SPECIFICITY;

EID: 9344261745     PISSN: 10399712     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (20)

1. Noda, L. (1973) The Enzymes, (Boyer, P. D., ed.) 3rd Ed., Vol.8, Academic Press, New York, 8, 279-305.
2. Kuby, S. A., (1991) A study of enzymes, Vol.1, Chapter 5, pp. 222-339, Hamada, M., Takenaka, H., Sumida, M., and Kuby, S. A., Vol. II, Chapter 17, pp. 403-444, CRC Press, Boca Raton, FL.
3. Hamada, M., Palmieri R. H., Russell G. A., and Kuby, S. A. (1979) Arch. Biochem. Biophys. 195, 155-177.
4. Fry, D. C., Kuby, S. A., and Mildvan, A. S. (1987) Biochemistry 26, 1645-1655.
5. Kim, H. J., Nishikawa, S., Tokutomi, Y., Takenaka, H., Hamada, M., Kuby, S. A., and Uesugi S. (1990) Biochemistry 29, 1107-1111.
6. Kim, K J., Nishikawa, S., Tanaka, T., Uesugi, S., Takenaka, H., Hamada, M., and Kuby, S. A. (1989) Protein Eng. 2, 379-386.
7. Pai, E. F., Sachsenheimer, W., Schirmer, R. H. and Schulz, G. E. (1977) J. Mol. Biol. 114, 37-45.
8. Kishi, F., Maruyama, M., Tanizawa, Y., and Nakazawa A. (1986) J. Biol. Chem. 261, 2942-2945.
9. Heil, A., Müller, G., Noda, L, Pinder, T., Schirmer, H., Schirmer, I., and von Zarbern, I., (1974) Eur. J. Chem. 43, 131-144.
10. Kuby, S. A., Hamada, M., Gerber, D., Tasi, W. C., Jacobs, H. K., Cress, M. C., Chua, G. K., Fleming, G., Wu, L. H., Fischer, A. H., Frischat, A., and Maland, L. (1978) Arch. Biochem. Biophys. 187, 34-52.
11. von Zabern, I., Wittmann-Liebold, B., Untucht-Grau, R., Schirmer, R. H. and Pai, E. F., (1976) Eur. J. Biochem. 68, 281-290.
12. Sambrook, J., Fritsch, E. F., and Maniatis, T., (1989) Molecular cloning, second ed.
13. Taylor, J. W., Ott, J., & Eckstein, F. (1985) Nucl. Acids Res., 13, 8764-8785.
14. Nakamaye, K., and Ecstein, F. (1986) Nucl. Acids Res. 14, 9679-9698.
15. Sayers, J. R., Krekel, C., and Eckstein, F. (1992) Biotechniques 13, 592-596.
16. Sanger, F., Nicklen, S., and Coullson, A. R., (1977) Proc. Natl. Acad. Sci. U.S.A. 74, 5463-5467.
17. Laemmli, U. K. (1970) Nature (London) 227, 680-685.
18. Lowry, O. H., Rosebrough, N. J., Farr, A. L, and Randall, R. J. (1951) J. Biol. Chem. 193, 265-275.
19. Heinkoff, S. (1984) Gene 28, 351-359.
20. Viera, J. and Messing, J., (1987) Method Enzymol. 153, 3-11.