The calcineurin target, Crz1, functions in azole tolerance but is not required for virulence of Candida albicans

Infection and Immunity

Volumn 72, Issue 12, 2004, Pages 7330-7333

  Onyewu, Chiatogu ^a   Wormley Jr , Floyd L ^a   Perfect, John R ^a   Heitman, Joseph ^a  

^a DUKE UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CALCINEURIN; FLUCONAZOLE; PROTEIN CRZ1; PYRROLE DERIVATIVE; TACROLIMUS; TRANSCRIPTION FACTOR; UNCLASSIFIED DRUG; ZINC FINGER PROTEIN;

ARTICLE; CANDIDA ALBICANS; CELL MEMBRANE; CELL SURVIVAL; CULTURE MEDIUM; DRUG SENSITIVITY; FUNGAL STRAIN; FUNGAL VIRULENCE; FUNGUS CULTURE; FUNGUS MUTANT; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION;

ANIMALS; ANTIFUNGAL AGENTS; CALCINEURIN; CANDIDA ALBICANS; DRUG RESISTANCE, FUNGAL; FLUCONAZOLE; MICE; MICE, INBRED ICR; TACROLIMUS;

EID: 9244263589     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/IAI.72.12.7330-7333.2004     Document Type: Article

References (21)

1. Bader, T., B. Bodendorfer, K. Schröppel, and J. Morscbhäuser. 2003. Calcineurin is essential for virulence in Candida albicans. Infect. Immun. 71: 5344-5354.
2. Beck-Sague, C. M., W. R. Jarvis, et al. 1993. Secular trends in the epidemiology of nosocomial fungal injections in the United States, 1980-1990. J. Infect. Dis. 167:1247-1251.
3. Blankenship, J. R., F. L. Wormley, M. K. Boyce, W. A. Schell, S. G. Filler, J. R. Perfect, and J. Heitman. 2003. Calcineurin is essential for Candida albicans survival in serum and virulence. Eukaryot. Cell 2:422-430.
4. Boustany, L. M., and M. S. Cyert. 2002. Calcineurin-dependent regulation of Crz1p nuclear export requires Msn5p and a conserved calcineurin docking site. Genes Dev. 16:608-619.
5. Bruno, V. M., and A. P. Mitchell. Regulation of azole drug susceptibility by Candida albicans protein kinase CK2. Mol. Microbiol., in press.
6. Cruz, M. C., A. L. Goldstein, J. R. Blankenship, M. Del Poeta, D. Davis, M. E. Cardenas, J. R. Perfect, J. H. McCusker, and J. Heitman. 2002. Calcineurin is essential for survival during membrane stress in Candida albicans. EMBO J. 21:546-559.
7. Davis, D., J. E. Edwards, Jr., A. P. Mitchell, and A. S. Ibrahim. 2000. Candida albicans RIM101 pH response pathway is required for host-pathogen interactions. Infect. Immun. 68:5953-5959.
8. Enloe, B., A. Diamond, and A. P. Mitchell. 2000. A single-transformation gene function test in diploid Candida albicans. J. Bacteriol. 182:5730-5736.
9. Heath, V. L., S. L. Shaw, S. Roy, and M. S. Cyert. 2004. Hph1p and Hph2p, novel components of calcineurin-mediated stress responses in Saccharomyces cerevisiae. Eukaryot. Cell 3:695-704.
10. Marchetti, O., J. M. Entenza, D. Sanglard, J. Bille, M. P. Glauser, and P. Moreillon. 2000. Fluconazole plus cyclosporine: a fungicidal combination effective against experimental endocarditis due to Candida albicans. Antimicrob. Agents Chemother. 44:2932-2938.
11. Marchetti, O., P. Moreillon, M. P. Glauser, J. Bille, and D. Sanglard. 2000. Potent synergism of the combination of fluconazole and cyclosporine in Candida albicans. Antimicrob. Agents Chemother. 44:2373-2381.
12. Matheos, D. P., T. J. Kingsbury, U. S. Ahsan, and K. W. Cunningham. 1997. Tcn1p/Crz1p, a calcineurin-dependent transcription factor that differentially regulates gene expression in Saccharomyces cerevisiae. Genes Dev. 11:3445-3458.
13. Mora-Duarte, J., R. Betts, C. Rotstein, A. L. Colombo, L. Thompson-Moya, J. Smietana, R. Lupinacci, C. Sable, N. Kartsonis, and J. R. Perfect for the Caspofungin Invasive Candidiasis Study Group. 2002. Comparison of caspofungin and amphotericin B for invasive candidiasis. N. Engl. J. Med. 347:2020-2029.
14. Odds, F. C. 1988. Candida and candidiasis: a review and bibliography, 2nd ed. Baillière Tindall, London, United Kingdom.
15. Onyewu, C., J. R. Blankenship, M. Del Poeta, and J. Heitman. 2003. Ergosterol biosynthesis inhibitors become fungicidal when combined with calcineurin inhibitors in Candida albicans, Candida glabrata, and Candida krusei. Antimicrob. Agents Chemother. 47:956-964.
16. Sanglard, D., F. Ischer, O. Marchetti, J. Entenza, and J. Bille. 2003. Calcineurin A of Candida albicans: involvement in antifungal tolerance, cell morphogenesis and virulence. Mol. Microbiol. 489:59-76.
17. Stathopoulos, A. M., and M. S. Cyert. 1997. Calcineurin acts through the CRZ1/TCN1-encoded transcription factor to regulate gene expression in yeast. Genes Dev. 11:3432-3444.
18. Stathopoulos-Gerontides, A., J. J. Guo, and M. S. Cyert. 1999. Yeast calcineurin regulates nuclear localization of the Crz1p transcription factor through dephosphorylation. Genes Dev. 13:798-803.
19. Walther, A., and J. Wendland. 2003. An improved transformation protocol for the human fungal pathogen Candida albicans. Curr. Genet. 42:339-343.
20. Wenzel, R. P. 1995. Nosocomial candidemia: risk factors and attributable mortality. Clin. Infect. Dis. 20:1531-1534.
21. Wilson, R. B., D. Davis, and A. P. Mitchell. 1999. Rapid hypothesis testing with Candida albicans through gene disruption with short homology regions. J. Bacteriol. 181:1868-1874.