Crystallization and preliminary x-ray crystallographic studies of ketosteroid isomerase from Pseudomonas putida biotype B

Proteins: Structure, Function and Genetics

Volumn 24, Issue 4, 1996, Pages 514-515

  Oh, Byung Ha ^a   Kim, Suhng Wook ^a   Ryu, Seong Eon ^b   Kim, Sang Soo ^c   Yoon, Mi Kyung ^c   Choi, Kwan Yong ^a  

^a Pohang University of Science and Technology (POSTECH)   (South Korea)

^b Korea Institute of Science and Technology   (South Korea)

^c Lucky Ltd   (South Korea)

Author keywords

crystallization; ketosteroid isomerase; protein steroid interaction

Indexed keywords

STEROID DELTA ISOMERASE;

ARTICLE; CRYSTAL STRUCTURE; CRYSTALLIZATION; ENZYME MECHANISM; ENZYME SUBSTRATE; ISOMERISM; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE; PSEUDOMONAS PUTIDA; SITE DIRECTED MUTAGENESIS; STEROID METABOLISM; X RAY CRYSTALLOGRAPHY;

CRYSTALLOGRAPHY, X-RAY; PSEUDOMONAS PUTIDA; STEROID ISOMERASES;

EID: 9244230026     PISSN: 08873585     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0134(199604)24:4<514::AID-PROT10>3.0.CO;2-O     Document Type: Article

References (19)

1. Fersht, A.R. "Enzyme Structure and Mechanism." 2nd edit. New York: Freeman, 1984.
2. Walsh, C. "Enzymatic Reaction Mechanisms." New York: Freeman, 1984.
3. 5-3-ketosteroid isomerase reaction: Catalytic mechanism, specificity, and inhibition. Adv. Enzyme Regul. 14:243-267, 1976.
4. Tamaoka, J., Ha, D.-M., Komagata, K. Reclassification of Pseudomonas acidovorans den Dooren de Jong 1926 and Pseudomonas testosteroni Marcus and Talalay 1956 as Comamonas acidovorans comb. nov. and Comamonas testosteroni comb, nov., with and emended description of the genus Comamonas. Int. J. Syst. Bacteriol. 37:52-59, 1987.
5. Benisek, W.F., Ogez, J.R., Smith, S.B. Recent studies of steroid isomerase from Pseudomonas testosteroni and Pseudomonas putida. Ann. N.Y. Acad. Sci. 346:115-130, 1980.
6. Schwab, J.M., Henserson, B.S. Enzyme-catalyzed allylic rearrangements. Chem. Rev. 90:1203-1245, 1990.
7. 5-3-Ketosteroid isomerase In: "The Enzyme VI." 3rd edit. Boyer, P.D. (ed.). New York: Academic Press, 1972:591-618.
8. 5-ketosteroid isomerase: Source of the catalytic power of the enzyme. Biochemistry 30:10849-10858, 1991.
9. 5-3-ketosteroid isomerase reaction by substrate, solvent, and combined kinetic deuterium isotope effects on wild-type and mutant enzymes. Biochemistry 29:7491-7300, 1990.
10. 5- 3-ketosteroid isomerase. Biochemistry 30:3169-3178, 1991.
11. 5-3- ketosteroid isomerase. Direct spectroscopic evidence for dienol intermediates. Biochemistry 30:4991-4997, 1991.
12. 5-3-ketoateroid isomerase by kinetic deuterium isotope effects. Biochemistry 30:10858-10865, 1991.
13. 5-3-ketosteroid isomerase: A new method of crystal density measurement. J. Mol. Biol. 103:659-664, 1976.
14. 5-3-ketosteroid isomerase. J. Biol. Chem. 259:9096-9103, 1984.
15. 5-3-ketosteroid isomerase from Pseudomonas putida. J. Bacteriol. 21:6672-6676, 1994.
16. 5-3-ketosteroid isomerase from Pseudomonas putida biotype B. J. Biol. Chem. 261:6454-6460, 1986.
17. 5-3-ketosteroid isomerase from Pseudomonas putida biotype B. J. Bacteriol. 177: 2602-2605, 1995.
18. Jancarik, Y., Kim, S.-H. Sparse matrix sampling: A screening method for crystallization of proteins. J. Applied Cryst. 24:409-411, 1991.
19. Matthews, B. Solvent content of protein crystals. J. Mol. Biol. 33:491-497, 1968.