A new organotypic culture method to study the actions of steroid hormones on the nervous system

Journal of Neuroscience Research

Volumn 43, Issue 6, 1996, Pages 719-725

  Levy, A ^a   Garcia Segura, L M ^c   Nevo, Z ^b   David, Y ^a   Naftolin, F ^d   Shahar, A ^a  

^a ISRAEL INSTITUTE FOR BIOLOGICAL RESEARCH   (Israel)

^b TEL AVIV UNIVERSITY   (Israel)

^c INSTITUTO CAJAL   (Spain)

^d YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

estrogen; hyaluronic acid; hypothalamus; slice cultures

Indexed keywords

ESTRADIOL; ESTROGEN; HYALURONIC ACID; STEROID HORMONE;

ANIMAL TISSUE; ARTICLE; BRAIN SLICE; CELL ULTRASTRUCTURE; EXTRACELLULAR MATRIX; FEMALE; HYPOTHALAMUS; MICROTUBULE; NERVOUS SYSTEM; NONHUMAN; ORGAN CULTURE; PRIORITY JOURNAL; RAT; SYNAPSE;

ANIMALS; CELL DIFFERENTIATION; CULTURE MEDIA; ESTRADIOL; FEMALE; FREEZE FRACTURING; HYALURONIC ACID; HYPOTHALAMUS; MICROSCOPY, ELECTRON; NERVOUS SYSTEM; ORGAN CULTURE TECHNIQUES; RATS; RATS, SPRAGUE-DAWLEY; STEROIDS; TESTOSTERONE;

EID: 9244225598     PISSN: 03604012     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-4547(19960315)43:6<719::AID-JNR8>3.0.CO;2-H     Document Type: Article

References (43)

1. Arnold AP. Gorski RA (1984): Gonadal steroid induction of structural sex differences in the central nervous system. Annu Rev Neurosci 7:413-442.
2. Asher R, Bignami A (1992): Hyaluronate binding and CD 44 expression in human glioblastoma cells and astrocytes. Exp Cell Res 203:80-90.
3. Banerjee SD, Toole BP (1991): Monoclonal antibody to chick embryo hyaluronate-binding protein: Changes in distribution of binding protein during early brain development. Dev Biol 146:186-197.
4. Bignami A, Asher R (1992): Some observations on the localization of hyaluronic acid in adult, newborn and embryonal rat brain. Int J Neurosci 10:45-57.
5. Bignami A, Perides G, Rahemtulla F (1993): Versican, a hyaluronate binding proteoglycan of embryonal precartilaginous mesenchyma, is mainly expressed postnatally in rat brain. J Neurosci Res 34:97-106.
6. Bornstein MB (1973): The immunopathology of demyelinative disorders examined. In Zimmerman HM (ed): "Organotypic Cultures of Mammalian Central Nervous Tissues: Progress in Neuropathology, Vol II." New York: Grune & Stratton, pp 69-90.
7. Bornstein MB, Murray MR (1958): Serial observations on patterns of growth, myelin formation, maintenance of degeneration in cultures of newborn rat kitten cerebellum. J Biophys Biochem Cytol 4:499-504.
8. Cabrol D, Huszar G, Romero R, Naftolin F (1981): Gestational changes in rat uterine cervix: Protein, collagen and glycosaminoglycan content. In: Dellwood DA, Anderson AM (eds): "The Cervix in Pregnancy and Labour." Edinburgh, Scotland: Churchill Livingstone, pp 34-39.
9. Diaz H, Lorenzo A, Carrer H, Caceres A (1992): Time lapse study of neunte growth in hypothalamus dissociated neurons in culture; sex differences and estrogen effect. J Neurosci Res 33:266-281.
10. Dohler KD (1991): The pre- and postnatal influence of hormones and neurotransmitters on sexual differentiation of the mammalian hypothalamus. Int Rev Cytol 131:1-57.
11. Ferreira A, Caceres A (1991): Estrogen enhanced neunte growth: Evidence for selective induction of tau and stable microtubules. J Neurosci 11:392-400.
12. Gahwiler BH (1984): Slice cultures of cerebellar, hippocampal and hypothalamic tissue. Experientia 40:235-243.
13. Garcia-Segura LM, Perrelet A (1984): Postsynaptic membrane domains in the molecular layer of the cerebellum: A correlation between presynaptic inputs and postsynaptic plasma membrane organization. Brain Res 321:255-266.
14. Garcia-Segura LM, Baetens D, Naftolin F (1985): Sex differences and maturational changes in arcuate nucleus neuronal plasma membrane organization. Dev Brain Res 19:146-149.
15. Garcia-Segura LM, Baetens D, Naftolin F (1986): Synaptic remodeling in arcuate nucleus after injection of estradiol valerate in adult female rats. Brain Res 366:131-136.
16. Garcia-Segura LM, Perez J, Tranque PA, Olmos G, Naftolin F (1988): Sexual differentiation of the neuronal plasma membrane: Neonatal levels of sex steroids modulate the number of exo-endocytotic images in the developing rat arcuate neurons. Neurosci Lett 91:19-23.
17. Goldberg AM (1987): Approaches to the development of in vitro neurotoxicological methods. In: Shahar A, Goldberg AM (eds): "Model Systems in Neurotoxicology: Alternative Approaches to Animal Testing." New York. Alan R. Liss, pp 1-11
18. Hauser KF, MacLusky NJ, Toran-Allerand D (1987): Androgen action in fetal mouse spinal cord cultures; metabolic and morphologic effects. Brain Res 406:62-72.
19. Hild W (1957): Myelogenesis in cultures of mammalian central nervous tissue. Zellforsch 46:71-95.
20. Hockfield S, Kalb RG, Zaremba S, Fryer H (1990): Expression of neuronal proteoglycans correlates with the acquisition of mature neuronal properties in the mammalian brain. Cold Spring Harbor Symp Quant Biol LV:505-514.
21. Jones KJ, Harrington CA, Chikaraishi DM, Pfaff W (1990). Steroid hormone regulation of ribosomal RNA in rat hypothalamus: early detection using in situ hybridization and precursor-product ribosomal DNA probes. J Neurochem 10:1513-1521
22. Kapfhammer JP, Schwab ME (1992): Modulators of neuronal migration and neunte growth. Curr Opin Cell Biol 4:863-868.
23. Leedy MG, Beattie MS, Bresnahan JC (1984): Testosterone-induced plasticity of synaptic input to adult mammalian motoneurons. Brain Res 424:386-390.
24. MacLusky NJ, Naftolin F (1981): Sexual differentiation of the central nervous system. Science 221:1294-1303.
25. Mann HB, Whitney DR (1947): On a test whether one or two random variables is statistically larger than the other. Ann Math Stat 18:50-60.
26. Naftolin F, Bruhlmann-Papazyan M, Baetens D, Garcia-Segura LM (1985): Neurons with whorl bodies have increased number of synapses. Brain Res 329:289-293.
27. Naftolin F, Garcia-Segura LM, Keefe D, Leranth C, MacLusky NJ, Brawer JR (1990): Estrogen effects on the synaptology and neural membranes of the rat hypothalamic arcuate nucleus. Bio Rep 42:21-28.
28. Notterpek LM, Bullock PN, Malek-Hedayet S, Fisher R, Rome LH (1993): Myelination in cerebellar slice cultures: Development of a system amenable to biochemical analysis. J Neurosci Res 36:621-634.
29. Olmos G, Naftolin F, Perez J, Tranque PA. Garcia-Segura LM (1989): Synaptic remodeling in the rat arcuate nucleus during the estrous cycle. Neuroscience 32:663-667.
30. Perez J, Naftolin F, Garcia-Segura LM (1990): Sexual differentiation of synaptic connectivity and neuronal plasma membrane in the arcuate nucleus of the rat hypothalamus. Brain Res 527:116-122.
31. Perez J, Hernandez P, Garcia-Segura LM (1991): Estradiol increases the number of nuclear pores in arcuate neurons of rat hypothalamus. J Comp Neurol 303:225-232.
32. Romijn HJ, de Jong BM, Ruijter JM (1988): A procedure for culturing rat neocortex explants in a serum free nutrient medium. J Neurosci Method 23:75-83.
33. Royet JP (1991): Stereology: A method for analyzing images. Prog Neurobiol 37:433-474.
34. Seil FJ (1979): Cerebellum in tissue culture. In Schneider DM (ed): "Reviews of Neuroscience. Vol 4." New York: Raven Press, pp 105-177.
35. Shahar A, Lustig S, Akov Y, David Y, Schneider P, Levin R (1991): Spinal cord slices with attached dorsal root ganglia: A culture model for the study of pathogenicity of encephalitic viruses. In Timiras PS, et al. (eds): "Plasticity and Regeneration of the Nervous System." New York: Plenum Press, pp 111-119.
36. Silberberg D, Benjamins J, Herschkowitz N, McKhann GM (1972): Incorporation of radioactive sulfate into sulfatide during myelination in cultures of rat cerebellum. J Neurochem 19:11-18.
37. Toran-Allerand CD (1976): Sex steroid and the development of the newborn mouse hypothalamus and preoptic area in vitro: Implication for sexual differentiation. Brain Res 106:407-412.
38. Toran-Allerand CD (1980): Sex steroids and the development of the newborn mouse hypothalamus and preoptic area in vitro. II. Morphological correlates and hormonal specificity. Brain Res 189:413-427.
39. Toran-Allerand CD (1984): On the genesis of sexual differentiation of the central nervous system: Morphometric consequences of steroidal exposure and possible role of α-feto protein. Prog Brain Res 61:63-98.
40. Vogel H, Butcher EC, Picker LJ (1992): H-CAM expression in the human nervous system: Evidence for a role in diverse glial interaction. J Neurocytol 21:363-373.
41. Walum E, Hansson E, Harvey AL (1990): In vitro testing of neurotoxicity. ATLA 18:153-179.
42. Witkin JW, Ferin M, Popilskis SJ, Silverman A-J (1991): Effects of gonadal steroids on the ultrastructure of GnRH neurons in the rhesus monkey: Synaptic input and glial apposition. Endocrinology 129:1083-1092.
43. Yasuhara O, Akiyama H, McGeer EG, McGeer PL (1994): Immunohistochemical localization of hyaluronic acid in rat and human brain. Brain Res 635:269-282.