Production of anti-CD2 chimeric antibody by recombinant animal cells

Journal of Bioscience and Bioengineering

Volumn 98, Issue 4, 2004, Pages 298-303

  Hotta, Akitsu ^a   Kamihira, Masamichi ^a   Itoh, Kanako ^a   Morshed, Mahboob ^a   Kawabe, Yoshinori ^a   Ono, Ken Ichiro ^a   Matsumoto, Hiroyuki ^a   Nishijima, Ken Ichi ^a   Iijima, Shinji ^a  

^a NAGOYA UNIVERSITY   (Japan)

Author keywords

Anti CD2; Chimeric antibody; Internal ribosomal entry site (IRES); Recombinant animal cell; Retroviral vector

Indexed keywords

DNA; GENES; GENETIC ENGINEERING; VIRUSES;

ANIMAL CELLS; ANTI-CD2 CHIMERIC ANTIBODIES;

ANTIBODIES;

CD20 ANTIBODY; CHIMERIC ANTIBODY; IMMUNOGLOBULIN HEAVY CHAIN; IMMUNOGLOBULIN LIGHT CHAIN; PLASMID DNA; RETROVIRUS VECTOR;

ANIMAL CELL; ANIMAL TISSUE; ANTIBODY PRODUCTION; ARTICLE; CHINESE HAMSTER; DEFECTIVE VIRUS; DNA REPLICATION; DNA TRANSFECTION; DNA VECTOR; ENCEPHALOMYOCARDITIS VIRUS; ENZYME LINKED IMMUNOSORBENT ASSAY; GENETIC TRANSDUCTION; INTERNAL RIBOSOME ENTRY SITE; MOUSE; NONHUMAN; VIRUS TRANSCRIPTION; WESTERN BLOTTING;

ANIMALIA; CRICETINAE; CRICETULUS GRISEUS; ENCEPHALOMYOCARDITIS VIRUS; UNIDENTIFIED RETROVIRUS;

EID: 9144271698     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1263/jbb.98.298     Document Type: Article

References (17)

1. Köhler, G. and Milstein, C.: Continuous cultures of fused cells secreting antibody of predefined specificity. Nature, 256, 495-497 (1975).
2. Brekke, O. H. and Sandlie, I.: Therapeutic antibodies for human diseases at the dawn of the twenty-first century. Nat. Rev. Drug Discov., 2, 52-62 (2003).
3. Kipriyanov, S. M. and Little, M.: Generation of recombinant antibodies. Mol. Biotechnol., 12, 173-201 (1999).
4. Rudolph, N. S.: Biopharmaceutical production in transgenic livestock. Trends Biotechnol., 17, 367-374 (1999).
5. Houdebine, L. M.: Transgenic animal bioreactors. Transgenic Res., 9, 305-320 (2000).
6. Ono, K., Kamihira, M., Kuga, Y., Matsumoto, H., Hotta, A., Itoh, T., Nishijima, K., and Iijima, S.: Production of anti-prion scFv-Fc fusion proteins by recombinant animal cells. J. Biosci. Bioeng., 95, 231-238 (2003).
7. Ghattas, I. R., Sanes, J. R., and Majors, J. E.: The encephalomyocarditis virus internal ribosome entry site allows efficient coexpression of two genes from a recombinant provirus in cultured cells and in embryos. Mol. Cell. Biol., 11, 5848-5859 (1991).
8. Mizuarai, S., Ono, K., Yamaguchi, K., Nishijima, K., Kamihira, M., and Iijima, S.: Production of transgenic quails with high frequency of germ-line transmission using VSV-G pseudotyped retroviral vector. Biochem. Biophys. Res. Commun., 286, 456-463 (2001).
9. Engvall, E. and Pearlmann, P.: Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry, 8, 871-874 (1971).
10. Smith, P. K., Krohn, R. I., Hermanson, G. T., Mallia, A. K., Gartner, F. H., Provenzano, M. D., Fujimoto, E. K., Goeke, N. M., Olson, B. J., and Klenk, D. C.: Measurement of protein using bicinchoninic acid. Anal. Biochem., 150, 76-85 (1985).
11. Laemmli, U. K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685 (1970).
12. Mizuguchi, H., Xu, Z., Ishii-Watabe, A., Uchida, E., and Hayakawa, T.: IRES-dependent second gene expression is significantly lower than cap-dependent first gene expression in a bicistronic vector. Mol. Ther., 1, 376-382 (2000).
13. Flasshove, M., Bardenheuer, W., Schneider, A., Hirsch, G., Bach, P., Bury, C., Moritz, T., Seeber, S., and Opalka, B.: Type and position of promoter elements in retroviral vectors have substantial effects on the expression level of an enhanced green fluorescent protein reporter gene. J. Cancer Res. Clin. Oncol., 126, 391-399 (2000).
14. Köhler, G.: Immunoglobulin chain loss in hybridoma lines. Proc. Natl. Acad. Sci. USA, 77, 2197-2199 (1980).
15. Kolb, A. F. and Siddell, S. G.: Expression of a recombinant monoclonal antibody from a bicistronic mRNA. Hybridoma, 16, 421-426 (1997).
16. Ngoi, S. M., Chien, A. C., and Lee, C. G.: Exploiting internal ribosome entry sites in gene therapy vector design. Curr. Gene Ther., 4, 15-31 (2004).
17. Gallego, J.: Internal initiation of translation by viral and cellular IRESs - a new avenue for specific inhibition of protein synthesis? Curr. Opin. Drug Discovery Dev., 5, 777-784 (2002).