Cloning and expression of an ether-bond-cleaving enzyme involved in the metabolism of polyethylene glycol

Journal of Bioscience and Bioengineering

Volumn 98, Issue 4, 2004, Pages 313-315

  Yamashita, Manabu ^a   Tani, Akio ^a   Kawai, Fusako ^a  

^a OKAYAMA UNIVERSITY   (Japan)

Author keywords

Ether bond cleaving enzyme; Metabolism of polyethylene glycol; Pseudonocardia sp. strain K1; Superoxide dismutase

Indexed keywords

AMINO ACIDS; CHEMICAL BONDS; CLONING; ESCHERICHIA COLI; GENES; GENETIC ENGINEERING; METABOLISM; POLYETHYLENE GLYCOLS;

ETHER-BOND-CLEAVING ENZYME; GENE ENCODING;

ENZYMES;

DIGLYCOLOIC ACID DEHYDROGENASE; ETHER; IMIDAZOLE; MACROGOL; OXIDOREDUCTASE; RECOMBINANT PROTEIN; SUPEROXIDE DISMUTASE; UNCLASSIFIED DRUG;

ACTINOBACTERIA; AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BACILLUS ANTHRACIS; COLORIMETRY; CONSENSUS SEQUENCE; DNA EXTRACTION; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE EXPRESSION; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PSEUDONOCARDIA; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY; STAPHYLOCOCCUS EPIDERMIDIS; THIN LAYER CHROMATOGRAPHY; XANTHOMONAS;

ACTINOBACTERIA (CLASS); BACILLUS ANTHRACIS; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; PROKARYOTA; PSEUDONOCARDIA; PSEUDONOCARDIA SP.; STAPHYLOCOCCUS EPIDERMIDIS; XANTHOMONAS;

EID: 9144224841     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1263/jbb.98.313     Document Type: Article

References (16)

1. Kawai, F.: Microbial degradation of polyethers. Appl. Microbiol. Biochem., 58, 30-38 (2002).
2. Yamashita, M., Tani, A., and Kawai, F.: A new ether bond-splitting enzyme found in gram-positive polyethylene glycol 6000-utilizing bacterium, Pseudonocardia sp. strain K1. Appl. Microbiol. Biochem., 10.1007/s00253-004- 1709-0 (October 12, 2004).
3. Kawai, R: The biochemistry of degradation of polyethers. Crit. Rev. Biotechnol., 6, 273-307 (1987).
4. White, G. F., Russell, N. J., and Tidswell, E. C.: Bacterial scission of ether bonds. Microbiol. Rev., 60, 216-232 (1996).
5. Read, T. D., Peterson, S. N., Tourasse, N., Baillie, L. W., Paulsen, I. T., Nelson, K. E., Tettelin, H., Fouts, D. E., Eisen, J. A., Gill, S. R., and other 42 authors: The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria. Nature, 423, 81-86 (2003).
6. Heidrich, C., Hantke, K., Bierbaum, G., and Sahl, H. G.: Identification and analysis of a gene encoding a Fur-like protein of Staphylococcus epidermidis. FEMS Microbiol. Lett., 140, 253-259 (1996).
7. Whitehouse, C. A., Williams, L. R., and Austin, F. E.: Identification of superoxide dismutase activity in Borrelia burgdorferi. Infect. Immun., 65, 4865-4868 (1997).
8. Marmur, J. A.: A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J. Mol. Biol., 3, 208-218 (1961).
9. Southern, E. M.: Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol., 98, 503-517 (1975).
10. Howard, O., Anne, S. G., and Daniel, L. H.: Genetic applications of an inverse polymerase chain reaction. Genetics, 120, 621-623 (1988).
11. Beauchamp, C. and Fridovich, I.: Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Annal. Biochem., 44, 276-287 (1971).
12. Rex, M. C. D., Daphne, C. E., William, H. E., and Kenneth, M. J.: Method for the detection of biochemical compounds on paper and thin layer chromatograms with some notes on separation, p. 453-497. In Rex, M. C. D., Daphne, C. E., William, H. E., and Kenneth, M. J. (ed.), Data for biochemical research. Clarendon Press, Oxford (1984).
13. Ling, J., Gao, X., Liu, W. Y., and Ruan, K.: DNA-cleaving activity of superoxide dismutases specific for circular super-coiled double-stranded DNA in vitro. Int. J. Biochem. Cell. Biol., 30, 1123-1127 (1998).
14. 2-induced DNA damage and alter its site specificity. FEBS Lett., 495, 187-190 (2001).
15. Cabelli, D. E., Allen, D., Bielski, B. H., and Holcman, J.: The interaction between Cu(I) superoxide dismutase and hydrogen peroxide. J. Biol. Chem., 264, 9967-9971 (1989).
16. Fridovich, I.: Superoxide dismutase. Annu. Rev. Biochem., 44, 147-159 (1975).