The in vivo bioactivity of vascular endothelial growth factor/vascular permeability factor is independent of N-linked glycosylation

Laboratory Investigation

Volumn 74, Issue 2, 1996, Pages 546-556

  Walter, Dirk H ^a   Hink, Uli ^a   Asahara, Takayuki ^a   Van Belle, Eric ^a   Horowitz, Jeffrey ^a   Tsurumi, Yukio ^a   Vandlen, Richard ^b   Heinsohn, Henry ^b   Keyt, Bruce ^b   Ferrara, Napoleone ^b   Symes, James F ^a   Isner, Jeffrey M ^a,c  

^a TUFTS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b GENENTECH INC   (United States)

^c ST ELIZABETH'S MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

VASCULOTROPIN;

AMINO TERMINAL SEQUENCE; ANGIOGENESIS; ANGIOGRAPHY; ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; BLOOD VESSEL REACTIVITY; CHO CELL; CONCENTRATION RESPONSE; DRUG ACTIVITY; GLYCOSYLATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; RABBIT; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

ANGIOGRAPHY; ANIMALS; BLOOD PRESSURE; CAPILLARIES; CELL DIVISION; CHO CELLS; CRICETINAE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDOTHELIAL GROWTH FACTORS; ENDOTHELIUM, VASCULAR; GLYCOSYLATION; LEG; LYMPHOKINES; PEPTIDE MAPPING; RABBITS; RECOMBINANT PROTEINS; REGIONAL BLOOD FLOW; VASCULAR ENDOTHELIAL GROWTH FACTOR A; VASCULAR ENDOTHELIAL GROWTH FACTORS;

EID: 9044251977     PISSN: 00236837     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (31)

1. Banai S, Jaklitsch MT, Shou M, Lazarous DF, Scheinowitz M, and Biro S (1994). Angiogenic-induced enhancement of collateral blood flow to ischemic myocardium by vascular endothelial growth factor in dogs. Circulation 89:2183-2189.
2. Bauters C, Asahara T, Zheng LP, Takeshita S, Bunting S, and Ferrara N (1994). Physiologic assessment of angiogenesis induced by vascular endothelial growth factor in a rabbit ischemic hindlimb model. Am J Physiol 36:H1263-H1271.
3. Bauters C, Asahara T, Zheng LP, Takeshita S, Bunting S, and Ferrara N (1995). Site-specific therapeutic angiogenesis after systemic administration of vascular endothelial growth factor. J Vasc Surg 21:314-325.
4. Claffey KP, Senger DR, and Spiegelman BM (1995). Structural requirements for dimerization, glycosylation, secretion, and biological function of VPF/VEGF. Biochim Biophys Acta 1246: 1-9.
5. Crestfield AM, Moore S, and Stein WJ (1963). The preparation and enzymatic hydrolysis of reduced and S-carboxymethylated proteins. J Biol Chem 238:622-627.
6. deVries C, Escobedo JA, Ueno H, Houck K, Ferrara N, and Williams LT (1992). The fms-like tyrosine kinase, a receptor for vascular endothelial growth factor. Science 255:989-991.
7. Ferrara N, and Henzel WJ (1989). Pituitary follicular cells secrete a novel heparin-binding growth factor specific for vascular endothelial cells. Biochem Biophys Res Commun 161:851-855.
8. Ferrara N, Leung DW, Cachianes G, Winer J, and Henzel WJ (1991). Purification and cloning of vascular endothelial growth factor secreted by pituitary follicolostellate cells. Methods Enzymol 198:391-404.
9. Ferrara N, Winer J, Burton T, Rowland A, Siegel M, and Phillips HS (1992). Expression of vascular endothelial growth factor does not promote transformation but confers a growth advantage in vivo to Chinese hamster ovary cells. J Clin Invest 91:160-170.
10. Ferrara N (1993). Vascular endothelial growth factor. Trends Cardiovasc Med 3:244-250.
11. Gitay-Goren H, Soker S, Vlodavsky I, and Neufeld G (1992). The binding of vascular endothelial growth factor to its receptors is dependent on cell surface-associated heparin-like molecules. J Biol Chem 267:6093-6098.
12. Houck KA, Ferrara N, Winer J, Cachianes G, Li B, and Leung DW (1991). The vascular endothelial growth factor family: Identification of a fourth molecular species and characterization of alternative splicing of RNA. Mol Endocrinol 5:1806-1814.
13. Houck KA, Leung DW, Rowland AM, Winer J, and Ferrara N (1992). Dual regulation of vascular endothelial growth factor bioavailability by genetic and proteolytic mechanisms. J Biol Chem 267:26031-26037.
14. Isner JM, Walsh K, Symes JF, Piezcek A, Takeshita S, and Lowry J (1995). Arterial gene therapy for therapeutic angiogenesis in patients with peripheral artery disease. Circulation 91:2687-2692.
15. Keck PJ, Hauser SD, Krivi G, Sanzo K, Warren T, and Feder J (1989). Vascular permeability factor, an endothelial cell mitogen related to PDGF. Science 246:1309-1312.
16. Kowallik P, Schulz R, Guth BD, Schade A, Paffhausen W, and Gross R (1991). Measurement of regional myocardial blood flow with multiple colored microspheres. Circulation 83:974-982.
17. Laemmli UK (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
18. Leung DW, Cachianes G, Kuang WJ, Goeddel DV, and Ferrara N (1989). Vascular endothelial growth factor is a secreted angiogenic mitogen. Science 246:1306-1309.
19. Mancini CBJ, Simon SB, McGillem MJ, LeFree MT, Friedman HZ, and Vogel RA (1987). Automated quantitative arteriography: Morphologic and physiologic validation in vivo of a rapid digital angiographic method. Circulation 75:452-460.
20. Miles AA, and Miles EM (1952). Vascular reactions to histamine, histamine liberators or leukotoxins in the skin of the guinea pig. J Physiol 118:228-257.
21. Millauer B, Wizigmann-Voos S, Schnurch H, Martinez R, Moller NPH, and Risau W (1993). High affinity VEGF binding and developmental expression suggest Flk-1 as a major regulator of vasculogenesis and angiogenesis. Cell 72:835-846.
22. Peretz D, Gitay-Goren H, Safran M, Kimmel N, Gospodarowicz D, and Neufeld G (1992). Glycosylation of vascular endothelial growth factor is not required for its mitogenic activity. Biochem Biophys Res Commun 182:1340-1347.
23. Ryan R, Charlesworth C, McCormick DJ, Milius RP, and Keutmann HT (1988). The glycoprotein hormones: Recent studies of structure-function relationships. FASEB J 2:2661-2669.
24. 2-macroglobulin and the binding is inhibited by heparin. J Biochem 7685-7691.
25. Stryer L (1988). Biochemistry, 3rd ed. New York, WH Freeman, 773.
26. Takeshita S, Zheng LP, Brogi E, Kearney M, Pu LQ, and Bunting S (1994). Therapeutic angiogenesis: A single intraarterial bolus of vascular endothelial growth factor augments revascularization in a rabbit ischemic hindlimb model. J Clin Invest 93:662-670.
27. Takeshita S, Rossow ST, Kearney M, Zheng LP, Bauters C, and Bunting S (1995). Time course of increased cellular proliferation in collateral arteries following administration of vascular endothelial growth factor in a rabbit model of lower limb vascular insufficiency. Am J Pathol 147:1649-1660.
28. Terman BI, Dougher-Vermazen M, Carrion ME, Dimitrov D, Armellino DC, and Gospodarowicz D (1992). Identification of the KDR tyrosine kinase as a receptor for vascular endothelial cell growth factor. Biochem Biophys Res Commun 187: 1579-1586.
29. Tischer E, Mitchell R, Hartmann T, Silva M, Gospodarowicz D, and Fiddes J (1991). The human gene for vascular endothelial growth factor: Multiple protein forms are encoded through alternative exon splicing. J Biol Chem 266:11947-11954.
30. Yeo T-K, Senger DR, Dvorak HF, Freter L, and Yeo K-T (1991). Glycosylation is essential for efficient secretion but not for permeability-enhancing activity of vascular permeability factor (vascular endothelial growth factor). Biochem Biophys Res Commun 179:1568-1575.
31. Ziada AM, Hudlicka O, Tyler KR, and Wright AJ (1984). The effect of long-term vasodilation on capillary growth and performance in rabbit heart and skeletal muscle. Cardiovasc Res 18:724-732.