Stably expressed antisense RNA to cytomegalovirus UL83 inhibits viral replication

Journal of Virology

Volumn 70, Issue 4, 1996, Pages 2086-2094

  Monte, Aola Dal ^a   Bessia, Christine ^a   Ripalti, Alessandro ^b   Landini, Maria Paola ^b   Topilko, Andrzej ^a   Plachter, Bodo ^c   Virelizier, Jean Louis ^a   Michelson, Susan ^a  

^a INSTITUT PASTEUR   (France)

^b UNIVERSITY OF BOLOGNA   (Italy)

^c UNIVERSITY HOSPITAL ERLANGEN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY RNA; PHOSPHOPROTEIN;

ARTICLE; ASTROCYTOMA CELL; CYTOMEGALOVIRUS; HUMAN; HUMAN CELL; IMMEDIATE EARLY GENE; OPEN READING FRAME; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN SYNTHESIS; TARGET CELL; VIRION; VIRUS EXPRESSION; VIRUS INHIBITION; VIRUS REPLICATION;

EID: 9044223653     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/jvi.70.4.2086-2094.1996     Document Type: Article

References (39)

1. Amadéi, C., M. Tardy-Panit, P. Couillin, M. Coulon, N. Caban, A. Boué, and S. Michelson. 1984. Kinetic study of the development and localization of human cytomegalovirus-induced antigens using monoclonal antibodies. Ann Virol. (Inst Pasteur) 134E:165-178.
2. Arbeit, R. D., P. L. Leary, and M. J. Levin. 1982 Gamma interferon production by combination of human peripheral blood lymphocytes, monocytes. and cultured macrophages Infect Immun. 35:383-390.
3. Arenzana-Seisdedos, F., J. L. Virelizier, and W. Fiers. 1985 Interferons as macrophage activating factors. III. Preferential effects of interferon-gamma on the interleukin secretion potential of fresh or aged human monocytes. J. Immunol. 134:2444-2448.
4. Azad, R. F., V. B. Driver, K. Tanaka, R. M. Cooke, and K. P. Anderson. 1993. Antiviral activity of a phosphorothioate oligonucleotide complementary to RNA of the human cytomegalovirus major immediate-early region. J. Virol. 37:1945-1954.
5. Bryant, L. A., and J. H. Sinclair. 1993. Inhibition of human cytomegalovirus major immediate early gene expression by antisense RNA expression vector. J. Gen. Virol. 74:1965-1967.
6. Cameron, F. H., and P. A. Jennings. 1991. Inhibition of gene expression by a short sense fragment. Nucleic Acids Res. 19:469-475.
7. Cantin, E. M., G. Pofsakoff, D. E. Wiley, and H. Openshaw. 1992. Antiviral effects of herpes simplex virus specific anti-sense nucleic acids. Adv. Exp. Med. Biol. 312:139-149.
8. Depto, A. S., and R. M. Stenberg. 1989. Regulated expression of the human cytomegalovirus pp65 gene: octamer sequence in the promoter is required for activation by viral gene products. J. Virol. 63:1232-1238.
9. Duclos, H., E. Elfassi, S. Michelson, F. Arenzana-Seisdedos, A. Munier, and J. L. Virelizier. 1989. Cytomegalovirus infection and transactivation of HIV-1 and HIV-2 LTRs in human astrocytoma cells. AIDS Res. Hum Retroviruses 5:217-224.
10. Gibson, W. 1983. Protein counterparts of human and simian cytomegaloviruses. Virology 128:391-406.
11. Grefte, J. M. M., B. T. F. van der Gun, S. Schmolke, M. van der Giessen, W. J. van Son, B. Plachter, G. Jahn, and T. H. The. 1992. The lower matrix protein pp65 is the principal viral antigen present in peripheral blood leukocytes during an active cytomegalovirus infection J. Gen. Virol. 73:2923-2932.
12. Gresser, I., M. T. Bandu, D. Brouty-Boye, and M. Tovey. 1974. Pronounced antiviral activity of human Interferon on bovine and porcine cells. Nature (London) 251:543-545.
13. Hensel, G. M., I. Buchmann, H. H. Meyer, S. Schmolke, B. Plachter, K. Radsak, and H. F. Kern. Intracellular localization and expression of the human cytomegalovirus (HCMV) matrix phosphoprotein pp71 (ppUL82): evidence for its translocation into the nucleus Submitted for publication.
14. Hirt, R. 1964. Selective extraction of polyoma DNA from infected mouse cell cultures. J. Mol Biol. 26:365-369.
15. Irmière, A., and W. Gibson. 1983. Isolation and characterization of a noninfectious virion-like particle released from cells infected with human strains of cytomegalovirus. Virology 130:118-133.
16. Kmeta, M. E., M. Ceruzzi, and J. Schwartz. 1991. Vmw65 phosphorothioate oligonucleotides inhibit HSV KOS replication and Vmw65 protein synthesis Antiviral Res. 16:173-184.
17. Kohler, C. P., J. A. Kerry, M. Cartier, V. P. Musithras, T. R. Jones, and R. M. Stenberg. 1994. Use of recombinant virus to assess human cytomegalovirus early and late promoters in the context of the viral genome. J. Virol. 68:6589-6597.
18. Kulka, M., C. C. Smith, L. Aurelian, R. Fishelevich, K. Meade, P. Miller, and P. O. Ts'o. 1989. Site specificity of the inhibitory effects of oligo(nucleoside methylphosphonate)s complementary to the acceptor splice junctions of herpes simplex virus type 1 immediate early mRNA 4. Proc. Natl. Acad. Sci USA 86:6868-6872.
19. Landini, M. P., B. Severi, G. Furlini, and L. Badiali. 1987. HCMV structural components: intracellular and intraviral localization of p28 and p65-69 by immunoelectron microscopy. Virus Res. 8:15-23.
20. Lindenmaier, W., A. Necker, S. Krause, R. Bonewald, and J. Collins. 1990. Cloning and characterization of the major antigenic determinants of human cytomegalovirus Ad169 seen by the immune system. Arch. Virol. 113:1-16.
21. Lin, B., and M. F. Stinski. 1992. Human cytomegalovirus contains a tegument protein that enhances transcription from promoters with upstream ATF and AP-1 cis-activing elements. J. Virol. 66:4434-4444.
22. Mazeron, M. C., G. Jahn, and B. Plachter. 1992. E13 monoclonal antibody to human cytomegalovirus recognizes several immediate early protein J Gen. Virol. 73:2699-2710.
23. 22a.Michelson, S. Unpublished data.
24. Michelson, S., M. Tardy-Panit, and O. Barzu. 1984. Properties of a human cytomegalovirus-induced protein kinase Virology 134:259-268.
25. Miller, A. D., and G. J. Rosman. 1989. Improved retroviral vectors for gene transfer and expression. J. Biotech. 7:980-990.
26. Pizzorno, M., P. O'Hare, L. Sha, R. L. LaFemina, and G. S. Hayward. 1988 trans-activation and autoregulation of gene expression by the immediate-early region 2 gene products of human cytomegalovirus. J. Virol. 62:1167-1179
27. Revello, M. G., E. Percivalle, A. DiMatteo, F. Morini, and G. Gerna. 1992 Nuclear expression of the lower matrix protein of human cytomegalovirus peripheral blood leukocytes of immunocompromised viraemic patients J Gen. Virol. 73:437-442
28. Riddell, S. R., M. J. Gilbert, C.-R. Li, B. A. Walter, and P. D. Greenberg. 1993. Reconstruction of protective CD8+ cytotoxic T lymphocyte responses to human cytomegalovirus in immunodeficient humans by adoptive transfer of T cell clones, p. 155-164. In S. Michelson and S. A. Plotkin (ed.), Multidisciplinary approach to understanding cytomegalovirus disease. International Congress Series vol 1032. Elsevier Science Publishers, Amsterdam
29. Ripalti, A., M. C. Boccuni, F. Campanini, and M. P. Landini. 1995. Cytomegalovirus-mediated induction of antisense mRNA expression to UL44 inhibits virus replication in an astrocytoma cell line: identification of an essential gene. J. Virol. 69:2047-2057.
30. Rüger, B., S. Klages, B. Walla, J. Albrecht, B. Fleckenstein, P. Tomlinson, and B. Barrell. 1987. Primary structure and transcription of the genes coding for the two virion phosphoproteins pp65 and pp71 of human cytomegalovirus. J. Virol. 61:446-453.
31. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
32. Scherczinger, C. A., and D. A. Knecht. 1993. Co-suppression of Dictoyostelium discoideum myosin II heavy-chain gene expression by a sense orientation transcript. Antisense Res. Dev. 3:207-217.
33. Schmolke, S., P. Drescher, G. Jahn, and B. Plachter. 1995. Nuclear targeting of the tegument protein pp65 (UL83) of human cytomegalovirus: an unusual bipartite nuclear localization signal function together with other portions of the protein to mediate its efficient nuclear transport. J. Virol. 69:1071-1078.
34. Schmolke, S., H. F. Kern, P. Drescher, G. Jahn, and B. Plachter. 1995. The dominant phosphoprotein pp65 (ppUL83) of human cytomegalovirus is dispensable for growth in cell culture. J. Virol. 69:5959-5568.
35. Smith, J. A., and G. S. Pari. 1995. Expression of human cytomegalovirus UL36 and UL37 genes is required for viral DNA synthesis. J. Virol. 69:1952-1931.
36. Somogyi, T., S. Michelson, and M. J. Masse. 1990. Genomic location of a human cytomegalovirus protein with protein kinase activity (PK68). Virology 174:276-285.
37. Spaete, R. R., R. C. Gehrz, and M. P. Landini. 1994. Human cytomegalovirus structural proteins J. Gen. Virol. 75:3287-3308.
38. Topilko, A., and S. Michelson. 1994. Morphological and cytochemical analysis of human cytomegalovirus inoculum: correlation of free particles in inoculum with counterparts in infected cells. Res Virol. 145:65-73.
39. Winkler, M., S. Schmolke, B. Plachter, and T. Stamminger. The UL69 protein of human cytomegalovirus (HCMV). a homologue of the herpes simplex virus ICP27. is contained within the tegument of virions and activates the major immediate-early enhancer of HCMV in synergy with the tegument protein pp71. Scand. J. Infect. Dis., in press.