Invertase secretion in Hansenula polymorpha under the AOX1 promoter from Pichia pastoris

Yeast

Volumn 12, Issue 9, 1996, Pages 815-822

  Rodriguez, L ^a   Narciandi, R E ^a   Roca, H ^a   Cremata, J ^a   Montesinos, R ^a   Rodriguez, E ^a   Grillo, J M ^a   Muzio, V ^a   Herrera, L S ^a   Delgado, J M ^a  

^a CENTER FOR GENETIC ENGINEERING AND BIOTECHNOLOGY   (Cuba)

Author keywords

Alcohol oxidase; AOX1; Fragment klenow; Hansenula autonomously replicating sequence; HARS; Pollk; Polymerase I; SUC2; Sucrose invertase

Indexed keywords

ALCOHOL OXIDASE; BETA FRUCTOFURANOSIDASE; DNA FRAGMENT;

ARTICLE; CONTROLLED STUDY; ENDOMYCETALES; ENZYME RELEASE; EXPRESSION VECTOR; HANSENULA POLYMORPHA; IMMUNOBLOTTING; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; SACCHAROMYCES CEREVISIAE;

ALCOHOL OXIDOREDUCTASES; BETA-FRUCTOFURANOSIDASE; CELL COMPARTMENTATION; CULTURE MEDIA; GENE EXPRESSION REGULATION, FUNGAL; GENES, FUNGAL; GENETIC VECTORS; GLYCEROL; GLYCOSIDE HYDROLASES; METHANOL; PICHIA; PROMOTER REGIONS (GENETICS); RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE;

PICHIA; PICHIA ANGUSTA; PICHIA PASTORIS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCETALES;

EID: 8944262345     PISSN: 0749503X     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0061(199607)12:9<815::AID-YEA916>3.0.CO;2-H     Document Type: Article

References (21)

1. Couderc, R. and Baratti, J. (1980). Oxidation of methanol by the yeast Pichia pastoris: purification and properties of alcohol oxidase. Agric. Biol. Chem. 44, 2279-2289.
2. Cregg, J. M., Tschopp, J. F., Stillman, C., et al. (1987). High level expression and efficient assembly of hepatitis B surface antigen in the methylotrophic yeast, Pichia pastoris. Bio/Technology 5, 479-485.
3. Digan, M. E., Lair, S. V., Brierley, R. A., et al. (1989). Continuous production of a novel lysosyme via secretion from the yeast Pichia pastoris. Biotechnology 7, 160-164.
4. Ellis, S. B., Brust, P. F., Koutz, P. J., Waters, A. F., Harpold, M. M. and Gingeras, T. R. (1985). Isolation of alcohol oxidase and two other methanol regulatable genes from the yeast Pichia pastoris. Mol. Cell. Biol. 5, 1111-1121.
5. Esmon, P. C., Esmon, B. E., Schauer, I. E., Taylor, A. and Schekman, R. (1987). Structure assembly and secretion of octameric invertase. J. Biol. Chem. 262, 4387-4394.
6. Gascon, S. and Lampen, J. O. (1968). Purification of the internal invertase of yeast. J. Biol. Chem. 243, 1567-1572.
7. Kaiser, G. A., Preuss, D., Grisafi, P. and Botstein, D. (1987). Many random sequences functionally replace the secretion signal sequence of yeast invertase. Science 235, 312-317.
8. Klebe, R. J., Harris, J. V., Sharp, Z. D. and Douglas, M. G. (1983). General method for polyethyleneglycol-induced genetic transformation of bacteria and yeast. Gene 25, 333-341.
9. Maniatis, T., Fritsch, E. F. and Sambrook, J. (1989). Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
10. Montesino, R., Cremata, J., Rodríguez, L. and Delgado, J. (1992). Low level of glycosylation of invertase secreted by methylotrophic yeast Hansenula polymorpha. Applied Biotechnology 9, 22-30.
11. Reddy, V., Johnson, R. S., Breman, K., et al. (1988). Characterization of the glycosilation sites in yeast external invertase. J. Biol. Chem. 263, 6978.
12. Roggenkamp, R., Hansen, H., Eckarct, M., Janowicz, Z. and Hollenberg, C. P. (1986). Transformation of the methylotrophic yeast Hansenula polymorpha by autonomous replication and integration vectors. Mol. Gen. Genet. 202, 302-308.
13. Sherman, F. and Fink, G. (1980). Manual of Yeast Genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
14. Sreekrishna, K., Tschopp, J. F. and Fuke, M. (1987). Invertase gene (SUC2) of Saccharomyces cerevisiae as a dominant marker for transformation of P. pastoris. Gene 59, 115-125.
15. Sreekrishna, K., Nelles, L., Potenz, R., et al. (1989). High-level, purification and characterisation of recombinant human tumour necrosis factor synthesised in the methylotrophic yeast Pichia pastoris. Biochemistry 28, 4117-4125.
16. Tammi, M., Ballou, L., Taylor, A. and Ballou, C. E. (1987). Effect of glycosylation on yeast invertase oligomer stability. J. Biol. Chem. 262, 4395-4401.
17. Tani, Y., Lim, W. J. and Yang, H. C. (1988). Isolation of L-methionine enriched mutant of a methylotrophic yeast Candida boidinii. J. Ferment. Technol. 66, 153-158.
18. Taussig, R. and Carlson, M. (1983). Nucleotide sequence of the yeast SUC2 gene for invertase. Nucl. Acids Res. 11, 1943-1954.
19. Tschopp, J. F., Brust, P. F., Cregg, J. M., Stillman, C. A. and Gingeras, T. R. (1987a). Expression of the lacZ gene from two methanol-regulated promoters in Pichia pastoris. Nucl. Acids Res. 15, 3859-3876.
20. Tschopp, J. F., Swerlow, G., Kosson, R., Craig, W. and Grinna, L. (1987b). High level secretion of glycosylated invertase in the methylotrophic yeast, Pichia pastoris. Bio/technology 5, 1305-1308.
21. Wegner, E. H. (1983). Biochemical conversions by yeast transformation at high cell densities. U.S. patent 4414329.