Detection of hepatitis C virus RNA in circulating immune complexes by RT-PCR

Hepato-Gastroenterology

Volumn 43, Issue 9, 1996, Pages 582-585

  Morita, Takechiyo ^a   Hada, Hajime ^a   Koide, Norio ^a   Shiraha, Hidenori ^a   Shinji, Toshiyuki ^a   Nakamura, Masaki ^a   Ujike, Kouzo ^a   Wato, Masaki ^a   Shimomura, Hiroyuki ^a   Tsuji, Takao ^a  

^a OKAYAMA UNIVERSITY MEDICAL SCHOOL   (Japan)

Author keywords

Circulating immune complex; Hepatitis C virus; RNA; RT PCR

Indexed keywords

IMMUNOGLOBULIN A ANTIBODY; IMMUNOGLOBULIN G ANTIBODY; IMMUNOGLOBULIN M ANTIBODY; VIRUS ENVELOPE PROTEIN; VIRUS RNA;

ANTIGEN ANTIBODY COMPLEX; ARTICLE; CHRONIC HEPATITIS; CLINICAL ARTICLE; HEPATITIS C; HEPATITIS C VIRUS; HUMAN; LIVER CIRRHOSIS; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SUPERNATANT;

ANTIGEN-ANTIBODY COMPLEX; HEPACIVIRUS; HEPATITIS C; HEPATITIS C ANTIBODIES; HEPATITIS, CHRONIC; HUMANS; LIVER CIRRHOSIS; POLYMERASE CHAIN REACTION; PRECIPITIN TESTS; RNA, VIRAL;

EID: 8944262041     PISSN: 01726390     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (15)

1. Choo Q-L, Kuo G, Weiner AJ, Overby LR, Bradley DW, Houghton M.: Isolation of a cDNA clone derived from a blood-borne non-A, non-B hepatitis genome. Science 1989; 21: 359-362.
2. Kuo G, Choo Q-L, Alter HJ, Gitmick GL, Redeker AG, Purcell RH, Miyamura T, Dienstag JL, Alter MJ, Stevens CE, Tegtmeier GE, Bonino F, Colombo M, Lee W-S, Kuo C, Berger K, Shuster JR, Overby LR, Bradley DW, Houghton M.: An assay for circulating antibodies to a major etiologic virus of human non-A, non-B hepatitis. Science 1989; 21: 362-364.
3. Houghton M, Weiner A, Han J, Kuo G, Choo Q-L.: Molecular biology of the hepatitis C viruses: implications for diagnosis, development and control of viral disease. Hepatology 1991; 14: 381-388.
4. Kato N, Sekiya H, Ootsuyama Y, Nakazawa T, Hijikata M, Ohkoshi S, Shimotohno K.: Humoral immune response to hypervariable region 1 of the putative envelope glycoprotein (gp70) of hepatitis C virus. J Virol 1993; 67: 3923-3930.
5. Dienstag JL, Bhan AK, Alter HJ, Feinstone SM, Purcell RH.: Circulating immune complexes in non-A, non-B hepatitis. Lancet 1979; i: 1265-1267.
6. Araki K, Nagashima H, Tsuji T.: Detection and characterization of circulating immune complexes during acute exacerbation of chronic viral hepatitis. Clin Exp Immunol 1982; 47: 520-526.
7. Hijikata M, Shimizu YK, Kato H, Iwamoto A, Shih JW, Alter HJ, Purcell RH, Yoshikura H.: Equilibrium centrifugation studies of hepatitis C virus: evidence for circulating immune complexes. J Virol 1993; 67: 1953-1958.
8. Kanto T, Hayashi N, Takehara T, Hagiwara H, Mita E, Naito M, Kasahara A, Fusamoto H, Kamada T.: Buoyant density of hepatitis C virus recovered from infected hosts: two different features in sucrose equilibrium density-gradient centrifugation related to degree of liver inflammation. Hepatology 1994; 19: 290-302.
9. Kanto T, Hayashi N, Takehara T, Hagiwara H, Mita E, Naito M, Kasahara A, Fusamoto H, Kamada T.: Density analysis of hepatitis C virus particle population in the circulation of infected hosts: implications for virus neutralization or persistence. J Hepatology 1995; 22: 440-448.
10. Chomczynski P, Sacchi N.: Single-step method of RNA isolation by guanidinium thiocyante-phenol-chloroform extraction. Anal Biochem 1987; 162: 156-159.
11. Okamoto H, Okada S, Sugiyama Y, Tanaka T, Sugai Y, Akahane Y, Machida A, Mishiro S, Yoshizawa H, Miyakawa Y, Mayumi M.: Detection of hepatitis C virus RNA by a two-stage polymerase chain reaction with two pairs of primers deduced from 5′-noncoding region. Jpn J Exp Med 1990; 60: 215-222.
12. Hada H, Koide N, Hanafusa T, Sakaguchi K, Shinji T, Sasaki S, Oka T, Takayama N, Yumoto Y, Tsuji T.: Detection by Western blotting of an antibody to the hepatitis C virus envelope protein in sera of patients with chronic liver disease. Acta Med Okayama 1992; 46: 365-370.
13. Johnson RJ, Gretch DR, Yamabe H, Hart J, Bacchi CE, Hartwell P, Couser WG, Corey L, Wener MH, Alpers CE, Wilson R.: Membranoproliferative glomerulonephritis associated with hepatitis C virus infection. N Engl J Med 1993; 328: 465-470.
14. Durand JM, Lefevre P, Harle JR, Boucrat J, Vitvitski L, Soubeyrand J.: Cutaneous vasculitis and cryoglobulinemia type II with hepatitis C virus infection. Lancet 1991; 337: 499-500.
15. Carson CW, Conn DL, Czaja AJ, Wright TL, Brecher ME.: Frequency and significance of antibodies to hepatitis C virus in polyarteritis nodosa. J Rheumatol 1993; 20: 304-309.