Clavin, a type-1 ribosome-inactivating protein from Aspergillus clavatus IFO 8605 - cDNA isolation, heterologous expression, biochemical and biological characterization of the recombinant protein

European Journal of Biochemistry

Volumn 239, Issue 2, 1996, Pages 272-280

  Parente, Dino ^a   Raucci, Giuseppe ^a   Celano, Bruna ^a   Pacilli, Aurelio ^a   Zanoni, Lorenzo ^a   Canevari, Silvana ^b   Adobati, Elena ^b   Colnaghi, Maria I ^b   Dosio, Franco ^c   Arpicco, Silvia ^c   Cattel, Luigi ^c   Mele, Antonio ^a   De Santis, Rita ^a  

^a Menarini Ricerche SpA   (Italy)

^b NATIONAL CANCER INSTITUTE   (Italy)

^c UNIVERSITY OF TURIN   (Italy)

Author keywords

Protein synthesis; Ribonuclease; Ribosome activating protein

Indexed keywords

COMPLEMENTARY DNA; MYCOTOXIN; RECOMBINANT PROTEIN; RIBONUCLEASE; RIBOSOME INACTIVATING PROTEIN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANTIBODY RESPONSE; ARTICLE; ASPERGILLUS; DRUG ACTIVITY; DRUG CLEARANCE; GENE EXPRESSION; GENE ISOLATION; IMMUNOGENICITY; LIVER TOXICITY; MOLECULAR CLONING; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN SYNTHESIS; SEQUENCE ANALYSIS;

ANIMALIA; ASPERGILLUS; ASPERGILLUS CLAVATUS; ESCHERICHIA COLI; FUNGI;

EID: 8944253778     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1996.0272u.x     Document Type: Article

References (38)

1. Arruda, L. K., Mann, B. J. & Chapman, M. D. (1992) Selective expression of a major allergen and cytotoxin, Asp fI in Aspergillus fumigatus, J. Immunol. 149, 3334-3359.
2. Bachmann, M., Blaser, P., Luthy, J. & Schlatter, C. (1992) Toxicity and mutagenicity of anthraquinones from Aspergillus chevalieri, J. Environ. Pathol. Toxicol. Oncol. 11, 49-52.
3. Barbieri, L. & Stirpe, F. (1982) Ribosome-inactivating proteins from plants: properties and possible uses, Cancer Surv. 1, 489-520.
4. Benigni, F., Canevari, S., Gadina, M., Adobati, E., Ferreri, A. J. M., Ferraris di Celle, E., Comolli, R. & Colnaghi, M. I. (1995) Preclinical evaluation of the ribosome inactivating proteins PAP-1, PAP-S and RTA in mice, Int. J. Immunopharmac. 17, 829-839.
5. Casalini, P., Caldera, M., Canevari, S., Ménard, S., Mezzanzanica, D., Tosi, E., Gadina, M. & Colnaghi, M. I. (1993) A critical comparison of three internalization assays applied to the evaluation of a given mAb as a toxin-carrier candidate, Cancer Immunol. Immunother. 37, 54-60.
6. Centis, F., Tagliabue, E., Uppungumduri, S., Pellegrini, R., Martignone, S., Mastroianni, A., Ménard, S. & Colnaghi, M. I. (1992) p185 Her2/ neu epitope mapping with murine monoclonal antibodies, Hybridoma 11, 267-276.
7. Conde, F. P., Orlandi, R., Canevari, S., Mezzanzanica, D., Ripamonti, M., Muñoz, S. M., Jorge, P. & Colnaghi, M. I. (1989) The Aspergillus toxin restrictocin is a suitable cytotoxic agent for generation of immunoconjugates with monoclonal antibodies directed against human carcinoma cells, Eur. J. Biochem. 178, 795-802.
8. Endo, Y. & Wool, I. G. (1982) The site of action of α-sarcin on eukaryotic ribosomes: the sequence at the α-sarcin cleavage site in 28S ribosomal ribonucleic acid, J. Biol. Chem. 257, 9054-9060.
9. Endo, Y., Chan, Y.-L., Lin, A., Tsurugi, K. & Wool, I. G. (1988) The cytotoxins α-sarcin and ricin retain their specificity when tested on a synthetic oligoribonucleotide (35-mer) that mimics a region of 28 S ribosomal ribonucleic acid, J. Biol. Chem. 263, 7917-7920.
10. Fernandez-Luna, J. L., Lopez-Otin, C., Soriano, E & Mendez, E. (1985) Complete amino acid sequence of the Aspergillus cytotoxin Mitogillin, Biochemistry 24, 861-867.
11. Ferreri, A. J. M., Ferraris di Celle, E., Caldera, M., Muñoz, S., Salvarelli, S., Coude, F. P., Canevari, S., Tosi, E. & Colnaghi, M. I. (1992) Production and characterization of a monoclonal antibody against the ribosome inactivating protein α-sarcin, Hybridoma 11, 437-446.
12. Fraker, P. J. & Speck, J. C. (1978) Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6-tetrachloro-3-α,6α-diphenylglycoluril, Biochem. Biophys. Res. Commun. 80, 849-857.
13. Friedman, P. N., Chace, D. F., Trail, P. A. & Siegall, C. B. (1993) Antitumor activity of the single-chain immunotoxin BR96 sFv-PE40 against established breast and lung tumor xenografts, J. Immunol. 150, 3054-3061.
14. Frohman, M. A. (1990) RACE: rapid amplification of cDNA ends, in PCR protocols (Innis, M. A., Gelfand, D. H., Sninsky, J. J. & Withe, T. J., eds) pp. 28-38, Academic Press, San Diego CA.
15. Gadina, M., Canevari, S., Ripamonti, M., Mariani, M. & Colnaghi, M. I. (1991) Preclinical pharmacokinetics and localization studies of the radioiodinated anti-ovarian carcinoma MAb MOv18, Int. J. Rad. Appl. Instrum. 18, 403-408.
16. Gadina, M., Tosi, E., Benigni, F., Mezzanzanica, D., Casalini, P., Canevari, S., Muñoz, S. M., Conde, F. P. & Colnaghi, M. I. (1993) Derivatization and pharmacokinetics of an anti EGF-R MAb and the RIP α-sarcin in the perspective of immunoconjugate generation, in Monoclonal antibodies application in clinical oncology (Epenetos, A. A., ed.) pp. 473-479, Chapman and Hall Medical, London UK.
17. Guttman, A., Cohen, A. S., Heiger, D. N. & Karger, B. L. (1990) Analytical and micropreparative ultrahigh resolution of oligonucleotides by polyacrylamide gel high-performance capillary electrophoresis, Anal. Chem. 62, 137-141.
18. Hansson, M., Ståhl, S., Hjorth, R., Uhlén, M. & Moks, T. (1994) Single-step recovery of a secreted recombinant protein by expanded bed adsorption. Bio/Technology 12, 285-288.
19. Henze, P.-P. C., Hahn, U., Erdmann, V. A. & Ulbrich, N. (1990) Expression of the chemically synthesized coding region for the cytotoxin α-sarcin in Escherichia coli using a secretion cloning vector, Eur. J. Biochem. 192, 127-131.
20. Hinnebusch, A. G. (1988) Mechanisms of gene regulation in the general control of amino acids biosynthesis in Saccharomyces cerevisiae, Microbiol. Rev. 52, 248-273.
21. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227, 680-685.
22. Lamy, B. & Davies, J. (1991) Isolation and nucleotide sequence of the Aspergillus restrictus gene coding for the ribonucleolytic toxin restrictocin and its expression in Aspergillus nidulans: The leader sequence protects producing strains from suicide, Nucleic Acids, Res. 19, 1001-1006.
23. Lohmer, S., Maddaloni, M., Motto, M., Salamini, F. & Thompson, R. D. (1993) Translation of the mRNA of the maize transcriptional activator opaque-2 is inhibited by upstream open reading frames present in the leader sequence, Plant Cell 5, 65-73.
24. Lopez-Otin, C., Barber, D., Fernandez-Luna, J. L., Soriano, F. & Mendez, E. (1984) The primary structure of the cytotoxin restrictocin, Eur. J. Biochem. 143, 621-634.
25. Manolov, R. J. (1992) Batch and continuous ribonuclease production by immobilized Aspergillus clavatus cells in a bubble-column bioreactor, Appl. Microbiol. Biotechnol. 37, 32-36.
26. Moks, T., Abrahmsén, L., Österlöf, B., Josephson, S., Östling, M., Enfors, S.-O., Persson, I., Nilsson, B. & Uhlén, M. (1987) Large-scale affinity purification of human insulin-like growth factor I from culture medium of Escherichia coli Bio/Technology 5, 379-382.
27. Nilsson, B., Moks, T., Jansson, B., Abrahmsén, L., Elmblad, A., Holmgren, E., Henrichson, C., Jones, A. & Uhlén, M. (1987) A synthetic IgG-binding domain based on staphylococcal protein A, Protein Eng. 1, 107-113.
28. Oka, T., Natori, Y., Tanaka, S., Tsurugi, K. & Endo, Y. (1990) Complete nucleotide sequence of cDNA for the cytotoxin α-sarcin, Nucleic Acids Res. 18, 1897.
29. Oka, T., Aoyama, Y., Natori, Y., Katano, T. & Endo, Y. (1992) An efficient expression system for a variant form of the cytotoxic protein α-sarcin in Escherichia coli, Biochim. Biophys. Acta 1130, 182-188.
30. Olson, B. H. & Goerner, G. L. (1965) α-Sarcin, a new antitumor agent. I. Isolation, purification, chemical composition, and the identity of a new amino acid, Appl. Microbiol. 13, 314-321.
31. Orlandi, R., Canevari, S., Conde, F. P., Leoni, F., Mezzanzanica, D., Ripamonti, M. & Colnaghi, M. I. (1988) Immunoconjugate generation between the ribosome inactivating protein restrictocin and an anti-human breast carcinoma MAb, Cancer Immunol, Immunother. 26, 114-120.
32. Quaas, R., McKeown, Y., Stanssens, P., Frank, R., Blocker, H. & Hahn, U. (1988) Expression of the chemically synthesized gene tor ribonuclease T1 in Escherichia coli using a secretion cloning vector, Eur. J. Biochem. 173, 617-622.
33. Soo-Kyung, O. H., Scott, M. P. & Sarnow, P. (1992) Homeotic gene Antennapedia mRNA contains 5′-noncoding sequences that confer translational initation by internal ribosome binding, Genes & Dev. 6, 1643-1653.
34. Stoessl, A. (1987) Microbial phytotoxin, in Handbook of microbiology (Laskin, A. I. & Lechevalier, H. A., eds) vol. VIII, pp. 171-236, CRC Press, Boca Raton FL.
35. Vitetta, E. S., Thorpe, P. E. & Uhr, J. W. (1993) Immunotoxins: magic bulletts or misguided missiles ? Immunol. Today 14, 252-259.
36. Wawrzynezak, E. J., Henry, R. V., Cumber, A. J., Parnell, G. D., Derbyshire, E. J. & Ulbrich, N. (1991) Biochemical, cytotoxic and pharmacokinetic properties of an immunotoxin composed of a mouse monoclonal antibody Fib 75 and the ribosome-inactivating protein α-sarcin from Aspergillus giganteus, Eur. J. Biochem. 196, 203-209.
37. Wnendt, S., Felske-Zech, H., Henze, P. P. C., Ulbrich, N. & Stahl, U. (1993) Characterization of the gene encoding α-sarcin, a ribosome-inactivating protein secreted by Aspergillus giganteus, Gene 124, 239-244.
38. Wool, I. G. (1984) The mechanism of action of the cytotoxic nuclease α-sarcin and its use to analyse ribosome structure, Trends Biochem. Sci. 9, 14-17.