Production of biologically-active recombinant goldfish gonadotropins in transgenic rainbow trout

Fish Physiology and Biochemistry

Volumn 28, Issue 1-4, 2003, Pages 473-474

  Morita, T ^a   Yoshizaki, Goro ^a   Kobayashi, M ^b   Takeuchi, T ^a  

^a TOKYO UNIVERSITY OF MARINE SCIENCE AND TECHNOLOGY   (Japan)

^b INTERNATIONAL CHRISTIAN UNIVERSITY   (Japan)

Author keywords

Bioreactor; Gonadotropin; Rainbow trout; Recombinant protein; Transgenic

Indexed keywords

ANTISERUM; BETA ACTIN; COMPLEMENTARY DNA; FOLLITROPIN BETA SUBUNIT; GLYCOPROTEIN; GONADOTROPIN; HYBRID PROTEIN; LUTEINIZING HORMONE BETA SUBUNIT; RECOMBINANT FOLLITROPIN; RECOMBINANT HORMONE; RECOMBINANT LUTEINIZING HORMONE;

ALPHA CHAIN; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; EMBRYO; FEASIBILITY STUDY; FEMALE; FERTILIZATION; GENE EXPRESSION SYSTEM; GENE FUSION; GENETIC CODE; GOLDFISH; HORMONE ACTION; HORMONE SYNTHESIS; IMMUNOREACTIVITY; IN VITRO STUDY; INCUBATION TIME; MALE; MICROINJECTION; NONHUMAN; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN GLYCOSYLATION; RAINBOW TROUT; TANDEM REPEAT; TRANSGENIC ANIMAL; WESTERN BLOTTING;

CARASSIUS AURATUS; ONCORHYNCHUS; ONCORHYNCHUS MYKISS; SALMONIDAE;

EID: 8844226595     PISSN: 09201742     EISSN: None     Source Type: Journal    
DOI: 10.1023/B:FISH.0000030632.41332.e8     Document Type: Article

References (3)

1. Kobayashi, M., Kato, Y., Yoshiura, Y. and Aida, K. 1997. Molecular cloning of the cDNA encoding two types of pituitary gonadotropin α subunit from the goldfish, Carassius auratus. Gen. Comp. Endocrinol. 105: 372-378.
2. Takagi, S., Sasado, T., Tamiya, G., Ozato, K., Wakamatsu, Y., Takeshita, A. and Kimura, M. 1994. An efficient expression vector for transgenic medaka construction. Mol. Mar. Biol. Biotechnol. 3: 192-199.
3. Yoshiura, Y., Kobayashi, M., Kato, Y. and Aida, K. 1997. Molecular cloning of the cDNAs encoding two gonadotropin β subunits (GTHIβ and IIβ) from the goldfish, Carassius auratus. Gen. Comp. Endocrinol. 105: 379-389.