A novel M-carbamoyl-L-amino acid amidohydrolase of Pseudomonas sp. strain ON-4a: Purification and characterization of M-carbamoyl-L-cysteine amidohydrolase expressed in Escherichia coli

Applied Microbiology and Biotechnology

Volumn 65, Issue 6, 2004, Pages 686-693

  Ohmachi, Tetsuo ^a   Narita, Megumi ^a   Kawata, Maki ^a   Bizen, Akiko ^a   Tamura, Yoshiharu ^b   Asada, Yoshihiro ^a  

^a HIROSAKI UNIVERSITY   (Japan)

^b Research Center   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; COLUMN CHROMATOGRAPHY; ELECTROPHORESIS; ENZYME INHIBITION; ESCHERICHIA COLI; GELS; GENES; HYDROLYSIS; PURIFICATION; SUBSTRATES;

L-CARBAMOLYASES; N-CARBOMOYL-L-CYSTEINE (NCC) AMIDOHYROLASE;

ENZYMES;

AMIDASE; BACTERIAL ENZYME; EDETIC ACID; MANGANESE; MERCURY; METAL ION; N CARBAMOYLCYSTEINE AMIDOHYDROLASE; UNCLASSIFIED DRUG; 1 CARBAMOYL L AMINO ACID AMIDOHYDROLASE; 1-CARBAMOYL-L-AMINO ACID AMIDOHYDROLASE; BACTERIAL PROTEIN; ENZYME INHIBITOR; PROTEIN SUBUNIT; RECOMBINANT PROTEIN;

ENZYME;

ANALYTIC METHOD; ARTICLE; BACTERIAL STRAIN; COLUMN CHROMATOGRAPHY; CONCENTRATION RESPONSE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME SUBSTRATE; ESCHERICHIA COLI; GEL FILTRATION; HYDROLYSIS; MOLECULAR WEIGHT; NONHUMAN; PH; PROTEIN EXPRESSION; PSEUDOMONAS; TEMPERATURE SENSITIVITY; CHEMISTRY; CHROMATOGRAPHY; COENZYME; ENZYME STABILITY; ENZYMOLOGY; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; STEREOISOMERISM; TEMPERATURE;

ESCHERICHIA COLI; PSEUDOMONAS; PSEUDOMONAS SP.;

AMIDOHYDROLASES; BACTERIAL PROTEINS; CHROMATOGRAPHY; CLONING, MOLECULAR; COENZYMES; EDETIC ACID; ENZYME INHIBITORS; ENZYME STABILITY; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; MANGANESE; MERCURY; MOLECULAR WEIGHT; PROTEIN SUBUNITS; PSEUDOMONAS; RECOMBINANT PROTEINS; STEREOISOMERISM; SUBSTRATE SPECIFICITY; TEMPERATURE;

EID: 8644265009     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-004-1687-2     Document Type: Article

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