Cloning, expression and characterization of l-asparaginase from Withania somnifera L. for large scale production

3 Biotech

Volumn 1, Issue 1, 2011, Pages 21-26

  Oza, Vishal P ^a   Parmar, Pritesh P ^a   Patel, Darshan H ^a   Subramanian, R B ^a  

^a SARDAR PATEL UNIVERSITY   (India)

Author keywords

cDNA; Cloning; Expression; l Asparaginase; Withania somnifera L.

Indexed keywords

ASPARAGINASE; COMPLEMENTARY DNA;

ARTICLE; CATALYSIS; DNA SYNTHESIS; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME SUBSTRATE; ESCHERICHIA COLI; GENE OVEREXPRESSION; GENE SEQUENCE; LARGE SCALE PRODUCTION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PRIORITY JOURNAL; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TEMPERATURE SENSITIVITY; WITHANIA SOMNIFERA;

MAMMALIA; WITHANIA SOMNIFERA;

EID: 85200168813     PISSN: 2190572X     EISSN: 21905738     Source Type: Journal    
DOI: 10.1007/s13205-011-0003-y     Document Type: Article

References (22)

1. +-dependent l-asparaginase. Planta 224: 668-679.
2. Duval M, Suciu S, Ferster A, Rialland X, Nelken B, Lutz P, Benoit Y, Robert A, Manel AM, Vilmer E, Otten J, Philippe N (2002) Comparison of Escherichia coli-asparaginase with Erwinia-asparaginase in the treatment of childhood lymphoid malignancies: results of a randomized European Organization for research and treatment of cancer-children's leukemia group phase 3 trial. Blood 99: 2734-2739.
3. Gilbert HJ, Blazek R, Bullman HMS, Minton NP (1986) Cloning and expression of the Erwinia chrysanthemi asparaginase gene in Escherichia coli and Erwinia carotovora. J Gen Microbiol 132: 151-160.
4. Graham ML (2003) Pegaspargase: a review of clinical studies. Adv Drug Deliv Rev 55: 1293-1302.
5. Hawkins DS, Park JR, Thomson BG, Felgenhauer JL, Holcenberg JS, Panosyan EH, Avramis VI (2004) Asparaginase pharmacokinetics after intensive polyethylene glycol-conjugated l-asparaginase therapy for children with relapsed acute lymphoblastic leukaemia. Clin Cancer Res 10: 5335-5341.
6. Howard JB, Carpenter FH (1972) l-Asparaginase from Erwinia carotovora. Substrate specificity and enzymatic properties. J Biol Chem 247: 1020-1030.
7. Karolina M, Grzegorz B, Mariusz J (2006) Crystal structure of plant asparaginase. J Mol Biol 360: 105-116.
8. Kotzia GA, Labrou NE (2005) Cloning, expression and characterization of Erwinia carotovoral-asparaginase. J Biotechnol 119: 309-323.
9. Kotzia GA, Labrou NE (2007) l-Asparaginase from Erwinia Chrysanthemi 3937: cloning, expression and characterization. J Biotechnol 127: 657-669.
10. Kozak M, Borek D, Janowski R, Jaskolski M (2002) Crystallization and preliminary crystallographic studies of five crystal forms of Escherichia colil-asparaginase II (Asp90Glu mutant). Acta Crystallogr D Biol Crystallogr 58: 130-132.
11. Kristiansen T, Einarsson M, Sundberg L, Porath J (1970) Purification of l-asparaginase from E. coli by specific adsorption and desorption. FEBS Lett 7: 294-296.
12. Lee SM, Wroble MH, Ross JT (1989) l-Asparaginase from Erwinia carotovora. An improved recovery and purification process using affinity chromatography. Appl Biochem Biotechnol 22: 1-11.
13. Liu JJ, Li J, Wu WT, Hu MQ (1996) Cloning and expression of the E. coli asparaginase gene in Escherichia coli. J Chin Pharm Univ 27: 696-700.
14. Lough TJ, Reddington BD, Grant MR, Hill DF, Reynolds PHS, Farnden KJF (1992) The isolation and characterization of a cDNA clone encoding l-asparaginase from developing seeds of Lupin (Lupinus arboreus). Plant Mol Biol 19: 391-399.
15. Lowry OH, Rosenbrough NJ, Farr LA, Randal RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193: 265-267.
16. Narta UK, Kanwar SS, Azmi W (2007) Pharmacological and clinical evaluation of l-asparaginase in the treatment of leukemia. Crit Rev Oncol Hematol 61: 208-221.
17. Oza VP, Trivedi SD, Parmar PP, Subramanian RB (2009) Withania somnifera L. (Ashwagandha): a novel source of l-asparaginase. J Integr Plant Biol 51: 201-206.
18. Oza VP, Kumar S, Parmar PP, Subramanian RB (2010) Anti-cancer properties of highly purified l-asparaginase from Withania somnifera L. against acute lymphoblastic leukemia. Appl Biochem Biotechnol 160: 1833-1840.
19. Sambrook J, Fritsch EF, Maniatis T (1989) In: Nolan C (ed) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor.
20. Sieciechowicz KA, Joy KW, Ireland RJ (1988) The metabolism of asparagine in plants. Phytochemistry 27: 663-671.
21. Sodek L, Lea PJ, Miflin DJ (1980) Distribution and properties of a potassium-dependent asparaginase isolated from developing seeds of Pisum sativum and other plants. Plant Physiol 65: 22-26.
22. Wriston JC Jr, Yellin TO (1973) l-Asparaginase: a review. Adv Enzymol Relat Areas Mol Biol 39: 185-248.