Failure to replicate: A sign of scientific misconduct?

Publications

Volumn 2, Issue 3, 2014, Pages 71-82

  Hill, Helene Z ^a   Pitt, Joel H ^b  

^a RUTGERS NEW JERSEY MEDICAL SCHOOL   (United States)

^b Renaissance Associates   (United States)

Author keywords

Experimental replication; Fabrication; Falsification; Research ethics; Research misconduct; Scientific retraction

Indexed keywords

EID: 85083545497     PISSN: None     EISSN: 23046775     Source Type: Journal    
DOI: 10.3390/publications2030071     Document Type: Article

References (31)

1. Bishayee, A.; Rao, D.V.; Howell, R.W. Evidence for pronounced bystander effects caused by nonuniform distributions of radioactivity using a novel three-dimensional tissue culture model. Radiat. Res. 1999, 152, 88–97.
2. Bishayee, A.; Hill, H.Z.; Stein, D.; Rao, D.V.; Howell, R.W. Free radical-initiated and gap junction-mediated bystander effect due to nonuniform distribution of incorporated radioactivity in a three-dimensional tissue culture model. Radiat. Res. 2001, 155, 335–344.
3. Tobey, R.A.; Anderson, E.C.; Petersen, D.F. The effect of thymidine on the duration of G1 in Chinese hamster cells. J. Cell Biol. 1967, 35, 53–59.
4. Puck, T.T. Studies of the Life Cycle of Mammalian Cells. Cold Spring Harb. Symp. Quant. Biol. 1964, 29, 167–176.
5. Bjursell, G.; Reichard, P. Effects of thymidine on deoxyribonucleoside triphosphate pools and deoxyribonucleic acid synthesis in Chinese hamster ovary cells. J. Biol. Chem. 1973, 248, 3904–3909.
6. Fox, R.M.; Tripp, E.H.; Tattersall, M.H. Mechanism of deoxycytidine rescue of thymidine toxicity in human T-leukemic lymphocytes. Cancer Res. 1980, 40, 1718–1721.
7. Hiramoto, K.; Narahara, K.; Kimoto, H. Synchronization culture of amniotic fluid cells using excess thymidine block followed by deoxycytidine release and its application to high-resolution banding analysis of chromosomes. Jpn. J. Hum. Genet. 1990, 35, 195–206.
8. Morris, N.R.; Fischer, G.A. Studies concerning inhibition of the synthesis of deoxycytidine by phosphorylated derivatives of thymidine. Biochim. Biophys. Acta 1960, 42, 183–184.
9. Wheater, R.F.; Roberts, S.H. An improved lymphocyte culture technique: Deoxycytidine release of a thymidine block and use of a constant humidity chamber for slide making. J. Med. Genet. 1987, 24, 113–114.
10. Ehmann, U.K.; Williams, J.R.; Nagle, W.A.; Brown, J.A.; Belli, J.A.; Lett, J.T. Perturbations in cell cycle progression from radioactive DNA precursors. Nature 1975, 258, 633–636.
11. Hoy, C.A.; Lewis, E.D.; Schimke, R.T. Perturbation of DNA replication and cell cycle progression by commonly used [3H]thymidine labeling protocols. Mol. Cell Biol. 1990, 10, 1584–1592.
12. Hu, V.W.; Black, G.E.; Torres-Duarte, A.; Abramson, F.P. 3H-thymidine is a defective tool with which to measure rates of DNA synthesis. FASEB J. 2002, 16, 1456–1457.
13. Pollack, A.; Bagwell, C.B.; Irvin, G.L., 3rd. Radiation from tritiated thymidine perturbs the cell cycle progression of stimulated lymphocytes. Science 1979, 203, 1025–1027.
14. Bedford, J.S.; Mitchell, J.B.; Griggs, H.G.; Bender, M.A. Cell killing by gamma rays and beta particles from tritiated water and incorporated tritiated thymidine. Radiat. Res. 1975, 63, 531–543.
15. Burki, H.J.; Koch, C.; Wolff, S. Molecular suicide studies of 125I and 3H disintegration in the DNA of Chinese hamster cells. Curr. Top. Radiat. Res. Q 1978, 12, 408–425.
16. Burki, H.J.; Okada, S. Killing of cultured mammalian cells by radioactive decay of tritiated thymidine at −196 °C. Radiat. Res. 1970, 41, 409–424.
17. Burki, H.J.; Roots, R.; Feinendegen, L.E.; Bond, V.P. Inactivation of mammalian cells after disintegration of 3H or 125I in cell DNA at −196 °C. Int. J. Radiat. Biol. Relat. Stud. Phys. Chem. Med. 1973, 24, 363–375.
18. Chan, P.C.; Lisco, E.; Lisco, H.; Adelstein, S.J. The radiotoxicity of iodine-125 in mammalian cells II. A comparative study on cell survival and cytogenetic responses to 125IUdR, 131TUdR, and 3HTdR. Radiat. Res. 1976, 67, 332–343.
19. Marin, G.; Bender, M.A. Survival Kinetics of Hela S-3 Cells after Incorporation of 3h-Thymidine or 3h-Uridine. Int. J. Radiat. Biol. Relat. Stud. Phys. Chem. Med. 1963, 7, 221–233.
20. Drew, R.M.; Painter, R.B. Action of tritiated thymidine on the clonal growth of mammalian cells. Radiat. Res. 1959, 11, 535–544.
21. Drew, R.M.; Painter, R.B. Further studies on the clonal growth of HeLa S3 cells treated with tritiated thymidine. Radiat. Res. 1962, 16, 303–311.
22. Keprtova, J.; Minarova, E. The effect of 3H-thymidine on the proliferation of in vitro cultured mammalian cells. Gen. Physiol. Biophys. 1985, 4, 81–92.
23. Persaud, R.; Zhou, H.; Baker, S.E.; Hei, T.K.; Hall, E.J. Assessment of low linear energy transfer radiation-induced bystander mutagenesis in a three-dimensional culture model. Cancer Res. 2005, 65, 9876–9882.
24. Panter, H.C. Cell inactivation by tritium decays at 37 and-196 degrees C: Some comparisons with X rays. Radiat. Res. 1981, 87, 79–89.
25. Buyse, M.; George, S.L.; Evans, S.; Geller, N.L.; Ranstam, J.; Scherrer, B.; Lesaffre, E.; Murray, G.; Edler, L.; Hutton, J.; et al. The role of biostatistics in the prevention, detection and treatment of fraud in clinical trials. Stat. Med. 1999, 18, 3435–3451.
26. Carlisle, J.B. The analysis of 168 randomised controlled trials to test data integrity. Anaesthesia 2012, 67, 521–537.
27. Hudes, M.L.; McCann, J.C.; Ames, B.N. Unusual clustering of coefficients of variation in published articles from a medical biochemistry department in India. FASEB J. 2009, 23, 689–703.
28. Al-Marzouki, S.; Evans, S.; Marshall, T.; Roberts, I. Are these data real? Statistical methods for the detection of data fabrication in clinical trials. BMJ 2005, 331, 267–270.
29. Baggerly, K.A.; Coombes, K.R. Deriving chemosensitivity from cell lines: Forensic bioinformatics and reproducible research in high-throughput biology. Ann. Appl. Stat. 2009, 3, 1309–1334.
30. Simonsohn, U. The lesson from two cases of fabricated data detected by statistics alone. Available online: http://ssrn.com/abstract=2114571 or http://dx.doi.org/10.2139/ssrn.2114571 (accessed on 21 November 2012).
31. Forensic Tools. Office of Research Integrity: Rockville MD, USA. Available online: http://ori.hhs.gov/forensic-tools (accessed on 26 August 2014)