On-line monitoring of physiological parameters in high cell density cultivations with recombinant E. coli

IFAC Proceedings Volumes (IFAC-PapersOnline)

Volumn 37, Issue 3, 2004, Pages 333-338

  Peuker, T ^a   Ellert, A ^a   Kaiser, C ^a   Lenz, K ^a   Eisholz, O ^a   Luttmann, R ^a  

^a UNIVERSITY OF APPLIED SCIENCES   (Germany)

Author keywords

Acetate; Ammonia; At line measurement of glucose; Cell specific reaction monitoring; E. coli; Flow analysis techniques; High cell density cultivation; MATLAB; OPC

Indexed keywords

AMMONIA; PHYSIOLOGICAL MODELS; SCADA SYSTEMS;

GREEN FLUORESCENCE PROTEINS; HIGH-CELL-DENSITY CULTIVATIONS; LINE MEASUREMENTS; MONITORING SYSTEM; NITROGEN SOURCES; ONLINE MONITORING; RECOMBINANT E. COLI; WILD-TYPE STRAIN;

ESCHERICHIA COLI;

EID: 85083232435     PISSN: 14746670     EISSN: None     Source Type: Conference Proceeding    
DOI: None     Document Type: Conference Paper

References (5)

1. Bech Jensen, E. and Carlsen, S. (1990): Production of recombinant human growth hormone in Escherichia coli: expression of different precursors and physiological effects of glucose and salts. Biotech. Bioeng., 36, pp. 1-11.
2. Luli, G. W. and Strahl, W. R. (1990): Comparison of growth, acetate production, and acetate inhibition of Escherichia coli strains in batch and fed-batch fermentations. Appl. Environ. Microbiol, 56, pp. 1004-1011.
3. Luttmann, R. and Gollmer, K. (2000): On-line Simulation Techniques for Bioreactor Control Development, in: K. Schügerl, K.-H. Bellgardt (eds): Bioreaction Engineering - Modeling and Control. Springer, Heidelberg, New York.
4. Riesenberg, D., Menzel, K., Schulz, V., Schumann, K., Veith, G., Zuber, G. and Knorre, W. A. (1990): High Cell Density Fermentation of Recombinant Escherichia coli Expressing Human Interferon Alphal. Appl. Microbiol. Biotech., 34, pp. 77-82.
5. Xu, B., Jahic, M., and Enfors, S.-O. (1999): Modeling of Overflow Metabolism in Batch and Fed-Batch Cultures of Escherichia coli, Biotechnol. Prog., 15, pp. 81-90