A fluorogenic peptide cleavage assay to screen for proteolytic activity: Applications for coronavirus spike protein activation

Journal of Visualized Experiments

Volumn 2019, Issue 143, 2019, Pages

  Jaimes, Javier A ^a,b   Millet, Jean K ^a,c   Goldstein, Monty E ^a,d   Whittaker, Gary R ^a   Straus, Marco R ^a  

^a Cornell University ^*  (United States)

^b INRA ^*  (United States)

^c INRA Institut National de la Recherche Agronomique   (France)

^d UNIVERSITY OF MARYLAND   (United States)

Author keywords

Biochemistry; Coronavirus; Feline coronavirus; Furin; Fusion peptide; Issue 143; Mers cov; Peptide assay; Protease cleavage

Indexed keywords

CORONAVIRUS SPIKE GLYCOPROTEIN; PEPTIDE;

ANIMAL; CAMEL; GENETICS; HUMAN; METABOLISM; MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS; PROTEIN DEGRADATION;

ANIMALS; CAMELUS; HUMANS; MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS; PEPTIDES; PROTEOLYSIS; SPIKE GLYCOPROTEIN, CORONAVIRUS;

EID: 85060610795     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/58892     Document Type: Article

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