Attenuated West Nile virus mutant NS1130-132QQA/175A/207A exhibits virus-induced ultrastructural changes and accumulation of protein in the endoplasmic reticulum

Journal of Virology

Volumn 89, Issue 2, 2015, Pages 1474-1478

  Whiteman, Melissa C ^a   Popov, Vsevolod ^b   Sherman, Michael B ^a   Wen, Julie ^b   Barrett, Alan D T ^a,b  

^a University of Texas Medical Branch School of Medicine   (United States)

^b UNIVERSITY OF TEXAS MEDICAL BRANCH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

NONSTRUCTURAL PROTEIN 1; NONSTRUCTURAL PROTEIN 4A; NONSTRUCTURAL PROTEIN 4B; NONSTRUCTURAL PROTEIN 5; PROTEIN DISULFIDE ISOMERASE; MUTANT PROTEIN; NONSTRUCTURAL PROTEIN 1, WEST NILE VIRUS; VIRAL PROTEIN;

3' UNTRANSLATED REGION; 5' UNTRANSLATED REGION; ARTICLE; CONTROLLED STUDY; ENDOPLASMIC RETICULUM; GOLGI COMPLEX; HUMAN; MONOLAYER CULTURE; PHENOTYPE; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN STRUCTURE; SITE DIRECTED MUTAGENESIS; TRANSMISSION ELECTRON MICROSCOPY; VIRUS ATTENUATION; VIRUS CAPSID; VIRUS ENVELOPE; VIRUS GENOME; VIRUS MUTANT; VIRUS REPLICATION; VIRUS STRAIN; WEST NILE FLAVIVIRUS; AMINO ACID SUBSTITUTION; ANIMAL; CELL ORGANELLE; CHEMISTRY; CHLOROCEBUS AETHIOPS; ELECTRON TOMOGRAPHY; FLUORESCENCE MICROSCOPY; GENETICS; GLYCOSYLATION; METABOLISM; MOUSE; PHYSIOLOGY; ULTRASTRUCTURE; VERO CELL LINE; WEST NILE VIRUS;

WEST NILE VIRUS;

AMINO ACID SUBSTITUTION; ANIMALS; CERCOPITHECUS AETHIOPS; ELECTRON MICROSCOPE TOMOGRAPHY; ENDOPLASMIC RETICULUM; GLYCOSYLATION; MICE; MICROSCOPY, ELECTRON, TRANSMISSION; MICROSCOPY, FLUORESCENCE; MUTANT PROTEINS; ORGANELLES; VERO CELLS; VIRAL NONSTRUCTURAL PROTEINS; VIRUS REPLICATION; WEST NILE VIRUS;

EID: 85047697253     PISSN: 0022538X     EISSN: 10985514     Source Type: Journal    
DOI: 10.1128/JVI.02215-14     Document Type: Article

References (22)

1. Whiteman MC, Li L, Wicker JA, Kinney RM, Huang C, Beasley DW, Chung KM, Diamond MS, Solomon T, Barrett AD. 2010. Development and characterization of non-glycosylated E and NS1 mutant viruses as a potential candidate vaccine for West Nile virus. Vaccine 28:1075-1083. http://dx.doi.org/10.1016/j.vaccine.2009.10.112.
2. Whiteman MC, Wicker JA, Kinney RM, Huang CY, Solomon T, Barrett AD. 2011. Multiple amino acid changes at the first glycosylation motif in NS1 protein of West Nile virus are necessary for complete attenuation for mouse neuroinvasiveness. Vaccine 29:9702-9710. http://dx.doi.org/10.1016/j.vaccine.2011.09.036.
3. Blitvich BJ, Scanlon D, Shiell BJ, Mackenzie JS, Pham K, Hall RA. 2001. Determination of the intramolecular disulfide bond arrangement and biochemical identification of the glycosylation sites of the nonstructural protein NS1 of Murray Valley encephalitis virus. J Gen Virol 82(Pt 9):2251-2256.
4. Flamand M, Megret F, Mathieu M, Lepault J, Rey FA, Deubel V. 1999. Dengue virus type 1 nonstructural glycoprotein NS1 is secreted from mammalian cells as a soluble hexamer in a glycosylation-dependent fashion. J Virol 73:6104-6110.
5. Mason PW. 1989. Maturation of Japanese encephalitis virus glycoproteins produced by infected mammalian and mosquito cells. Virology 169: 354-364. http://dx.doi.org/10.1016/0042-6822(89)90161-X.
6. Schlesinger JJ, Brandriss MW, Putnak JR, Walsh EE. 1990. Cell surface expression of yellow fever virus non-structural glycoprotein NS1: consequences of interaction with antibody. J Gen Virol 71(Pt 3):593-599. http://dx.doi.org/10.1099/0022-1317-71-3-593.
7. Winkler G, Randolph VB, Cleaves GR, Ryan TE, Stollar V. 1988. Evidence that the mature form of the flavivirus nonstructural protein NS1 is a dimer. Virology 162:187-196. http://dx.doi.org/10.1016/0042-6822(88)90408-4.
8. Winkler G, Maxwell SE, Ruemmler C, Stollar V. 1989. Newly synthesized dengue-2 virus nonstructural protein NS1 is a soluble protein but becomes partially hydrophobic and membrane-associated after dimerization. Virology 171:302-305. http://dx.doi.org/10.1016/0042-6822(89)90544-8.
9. Crooks AJ, Lee JM, Easterbrook LM, Timofeev AV, Stephenson JR. 1994. The NS1 protein of tick-borne encephalitis virus forms multimeric species upon secretion from the host cell. J Gen Virol 75(Pt 12):3453-3460. http://dx.doi.org/10.1099/0022-1317-75-12-3453.
10. Post PR, Carvalho R, Galler R. 1991. Glycosylation and secretion of yellow fever virus nonstructural protein NS1. Virus Res 18:291-302. http://dx.doi.org/10.1016/0168-1702(91)90025-Q.
11. Beasley DW, Holbrook MR, Travassos Da Rosa AP, Coffey L, Carrara AS, Phillippi-Falkenstein K, Bohm RP, Ratterree MS, Lillibridge KM, Ludwig GV, Estrada-Franco J, Weaver SC, Tesh RB, Shope RE, Barrett AD. 2004. Use of a recombinant envelope protein subunit antigen for specific serological diagnosis of West Nile virus infection. J Clin Microbiol 42:2759-2765. http://dx.doi.org/10.1128/JCM.42.6.2759-2765.2004.
12. Lindenbach BD, Rice CM. 1997. trans-complementation of yellow fever virus NS1 reveals a role in early RNA replication. J Virol 71:9608-9617.
13. Lindenbach BD, Rice CM. 1999. Genetic interaction of flavivirus nonstructural proteins NS1 and NS4A as a determinant of replicase function. J Virol 73:4611-4621.
14. Gillespie LK, Hoenen A, Morgan G, Mackenzie JM. 2010. The endoplasmic reticulum provides the membrane platform for biogenesis of the flavivirus replication complex. J Virol 84:10438-10447. http://dx.doi.org/10.1128/JVI.00986-10.
15. Mackenzie JM, Jones MK, Westaway EG. 1999. Markers for trans-Golgi membranes and the intermediate compartment localize to induced membranes with distinct replication functions in flavivirus-infected cells. J Virol 73:9555-9567.
16. Kaufusi PH, Kelley JF, Yanagihara R, Nerurkar VR. 2014. Induction of endoplasmic reticulum-derived replication-competent membrane structures by West Nile virus non-structural protein 4B. PLoS One 9:e84040. http://dx.doi.org/10.1371/journal.pone.0084040.
17. Westaway EG, Mackenzie JM, Kenney MT, Jones MK, Khromykh AA. 1997. Ultrastructure of Kunjin virus-infected cells: colocalization of NS1 and NS3 with double-stranded RNA, and of NS2B with NS3, in virusinduced membrane structures. J Virol 71:6650-6661.
18. Offerdahl DK, Dorward DW, Hansen BT, Bloom ME. 2012. A threedimensional comparison of tick-borne flavivirus infection in mammalian and tick cell lines. PLoS One 7:e47912. http://dx.doi.org/10.1371/journal.pone.0047912.
19. Leary K, Blair CD. 1980. Sequential events in the morphogenesis of Japanese encephalitis virus. J Ultrastruct Res 72:123-129. http://dx.doi.org/10.1016/S0022-5320(80)90050-7.
20. den Boon JA, Ahlquist P. 2010. Organelle-like membrane compartmentalization of positive-strand RNA virus replication factories. Annu Rev Microbiol 64:241-256. http://dx.doi.org/10.1146/annurev.micro.112408.134012.
21. Mastronarde DN. 2005. Automated electron microscope tomography using robust prediction of specimen movements. J Struct Biol 152:36-51. http://dx.doi.org/10.1016/j.jsb.2005.07.007.
22. Kremer JR, Mastronarde DN, McIntosh JR. 1996. Computer visualization of three-dimensional image data using IMOD. J Struct Biol 116:71-76. http://dx.doi.org/10.1006/jsbi.1996.0013.