Evaluation of Consistency in Spheroid Invasion Assays

Scientific Reports

Volumn 6, Issue , 2016, Pages

  Castillo, Liliana R Cisneros ^a   Oancea, Andrei Dumitru ^b   Stüllein, Christian ^b   Régnier Vigouroux, Anne ^a  

^a JOHANNES GUTENBERG UNIVERSITY   (Germany)

^b CLADIAC GmbH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

COLLAGEN;

CELL CULTURE TECHNIQUE; CELL MOTION; CELL PROLIFERATION; CHEMISTRY; CYTOLOGY; DEVICES; HUMAN; MULTICELLULAR SPHEROID; PATHOLOGY; REPRODUCIBILITY; STATISTICAL MODEL; TUMOR CELL CULTURE; TUMOR CELL LINE; TUMOR INVASION;

CELL CULTURE TECHNIQUES; CELL LINE, TUMOR; CELL MOVEMENT; CELL PROLIFERATION; COLLAGEN; HUMANS; MODELS, STATISTICAL; NEOPLASM INVASIVENESS; REPRODUCIBILITY OF RESULTS; SPHEROIDS, CELLULAR; TUMOR CELLS, CULTURED;

EID: 85046278476     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep28375     Document Type: Article

References (18)

1. Werb, Z. & Lu, P. The Role of Stroma in Tumor Development. Cancer J. 21, 250-253 (2015).
2. Gutmann, D. H. Microglia in the tumor microenvironment: taking their TOLL on glioma biology. Neuro Oncol. 17, 171-173 (2015).
3. Lorger, M. Tumor microenvironment in the brain. Cancers (Basel) 4, 218-243 (2012).
4. Ivanov, D. P. et al. Multiplexing spheroid volume, resazurin and acid phosphatase viability assays for high-throughput screening of tumour spheroids and stem cell neurospheres. PLoS One 9, e103817 (2014).
5. Birgersdotter, A., Sandberg, R. & Ernberg, I. Gene expression perturbation in vitro - a growing case for three-dimensional (3D) culture systems. Semin. Cancer Biol. 15, 405-412 (2005).
6. Fitzgerald, K. A., Malhotra, M., Curtin, C. M., O'Brien, F. J. & Driscoll, C. M. Life in 3D is never flat: 3D models to optimise drug delivery. J. Control Release 215, 39-54 (2015).
7. Vinci, M. et al. Advances in establishment and analysis of three-dimensional tumor spheroid-based functional assays for target validation and drug evaluation. BMC Biol. 10, 29 (2012).
8. Kunz-Schughart, L. A., Freyer, J. P., Hofstaedter, F. & Ebner, R. The use of 3-D cultures for high-throughput screening: the multicellular spheroid model. J. Biomol. Screen 9, 273-285 (2004).
9. Beaumont, K., Mohana-Kumaran, N. & Haass, N. Modeling Melanoma In Vitro and In Vivo. Healthcare 2, 27-46 (2013).
10. Wenzel, C. et al. 3D high-content screening for the identification of compounds that target cells in dormant tumor spheroid regions. Exp. Cell Res. 323, 131-143 (2014).
11. Seliger, C. et al. Lactate-modulated induction of THBS-1 activates transforming growth factor (TGF)-beta2 and migration of glioma cells in vitro. PLoS One 8, e78935 (2013).
12. Baumann, F. et al. Lactate promotes glioma migration by TGF-beta2-dependent regulation of matrix metalloproteinase-2. Neuro Oncol. 11, 368-380 (2009).
13. Zhuang, T. et al. Involvement of nitric oxide synthase in matrix metalloproteinase-9-and/or urokinase plasminogen activator receptor-mediated glioma cell migration. BMC Cancer 13, 590 (2013).
14. Koshkin, V., Ailles, L. E., Liu, G. & Krylov, S. N. Metabolic suppression of a drug resistant subpopulation in cancer spheroid cells. J. Cell Biochem. doi: 10.1002/jcb.25247 (2015).
15. Stein, A. M., Demuth, T., Mobley, D., Berens, M. & Sander, L. M. A mathematical model of glioblastoma tumor spheroid invasion in a three-dimensional in vitro experiment. Biophys. J. 92, 356-365 (2007).
16. Cheng, V., Esteves, F., Chakrabarty, A., Cockle, J., Short, S. & Bruning-Richardson, A. High-content analysis of tumour cell invasion in three-dimensional spheroid assays. Oncoscience 2, 596-606 (2015).
17. Del Duca, D., Werbowetski, T. & Del Maestro, R. F. Spheroid preparation from hanging drops: characterization of a model of brain tumor invasion. J. Neurooncol. 67, 295-303 (2004).
18. Noack, J., Choi, J., Richter, K., Kopp-Schneider, A. & Régnier-Vigouroux, A. A sphingosine kinase inhibitor combined with temozolomide induces glioblastoma cell death through accumulation of dihydrosphingosine and dihydroceramide, endoplasmic reticulum stress and autophagy. Cell Death & Disease 5, e1425 (2014).