Randomization of genes by PCR mutagenesis

Genome Research

Volumn 2, Issue 1, 1992, Pages 28-33

  Cadwell, R Craig ^a   Joyce, Gerald F ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; PROTOZOAL PROTEIN; RECOMBINANT DNA; RIBOZYME;

ANIMAL; ARTICLE; GENE; GENETICS; METHODOLOGY; MOLECULAR GENETICS; MUTAGENESIS; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; RANDOMIZATION; TETRAHYMENA;

ANIMALS; BASE SEQUENCE; DNA; DNA, RECOMBINANT; GENES, PROTOZOAN; MOLECULAR SEQUENCE DATA; MUTAGENESIS; POLYMERASE CHAIN REACTION; PROTOZOAN PROTEINS; RANDOM ALLOCATION; RNA, CATALYTIC; TETRAHYMENA;

EID: 85045502002     PISSN: 10889051     EISSN: None     Source Type: Journal    
DOI: 10.1101/gr.2.1.28     Document Type: Article

References (20)

1. Chu, C.-T., D.S. Parris, R.A.F. Dixon, F.E. Farber, and P.A. Schaffer. 1979. Hydroxylamine mutagenesis of HSV DNA and DNA fragments: Introduction of mutations into selected regions of the viral genome. Virology 98: 168-181.
2. Shortle, D. and D. Botstein. 1983. Directed mutagenesis with sodium bisulfite. Methods Enzymol. 100: 457-468.
3. Myers, R.M., L.S. Lerman, and T. Maniatis. 1985. A general method for saturation mutagenesis of cloned DNA fragments. Science 229: 242-247.
4. Diaz, J.-J., D.D. Rhoads, and D.J. Roufa. 1991. PCR-mediated chemical mutagenesis of cloned duplex DNAs. BioTechniques 11: 204-211.
5. Flavell, R.A., D.L. Sabo, E.F. Bandle, and C. Weissmann. 1974. Site-directed mutagenesis: Generation of an extracistronic mutation in bacteriophage Qβ RNA. J. Mol. Biol. 89: 255-272.
6. Mueller, W., H. Weber, F. Meyer, and C. Weissmann. 1978. Site-directed mutagenesis in DNA: Generation of point mutations in cloned β-globin complementary DNA at the positions corresponding to amino acids 121 to 123. J. Mol. Biol. 124: 343-358.
7. Mott, J.E., J. van Arsdell, and T. Platt. 1984. Targeted mutagenesis in vitro: lac repressor mutations generated using AMV reverse transcriptase and dBrUTP. Nucleic Acids Res. 12: 4139-4152.
8. Cox, E.C. 1976. Bacterial mutator genes and the control of spontaneous mutation. Annu. Rev. Genet. 10: 135-156.
9. Matteucci, M.D. and H.L. Heyneker. 1983. Targeted random mutagenesis: The use of ambiguously synthesized oligonucleotides to mutagenize sequences immediately 5′ of an ATG initiation codon. Nucleic Acids Res. 11: 3113-3121.
10. Hui, A., J. Hayflick, K. Dinkelspiel, and H.A. de Boer. 1984. Mutagenesis of the three bases preceding the start codon of the β-galactosidase mRNA and its effect on translation in Escherichia coli. EMBO J. 3: 623-629.
11. Dreher, T.W., J.J. Bujarski, and T.C. Hall. 1984. Mutant viral RNAs synthesized in vitro show altered aminoacylation and replicase activities. Nature 311: 171-175.
12. Wells, J.A., M. Vasser, and D.B. Powers. 1985. Cassette mutagenesis: An efficient method for generation of multiple mutations at defined sites. Gene 34: 315-323.
13. McNeil, J.B. and M. Smith. 1985. Saccharomyces cerevisiae CYC1 mRNA 5′-end positioning: Analysis by in vitro mutagenesis using synthetic duplexes with random mismatch base pairs. Mol. Cell. Biol. 5: 3545-3551.
14. Oliphant, A.R., A.L. Nussbaum, and K. Struhl. 1986. Cloning of random-sequence oligodeoxynucleotides. Gene 44: 177-183.
15. Horwitz, M.S.Z. and LA. Loeb. 1986. Promoters selected from random DNA sequences. Proc. Natl. Acad. Sci. 83: 7405-7409.
16. Hutchison, C.A., S.K. Nordeen, K. Vogt, and M.H. Edgell. 1986. A complete library of point substitution mutations in the glucocorticoid response element of mouse mammary tumor virus. Proc. Natl. Acad. Sci. 83: 710-714.
17. Derbyshire, K.M., J.J. Salvo, and N.D.F. Grindley. 1986. A simple and efficient procedure for saturation mutagenesis using mixed oligodeoxynucleotides. Gene 46: 145-152.
18. Ner, S.S., D.B. Goodin, and M. Smith. 1988. A simple and efficient procedure for generating random point mutations and for codon replacements using mixed oligonucleotides. DNA 7: 127-134.
19. Reidhaar-Olson, J.F. and R.T. Sauer. 1988. Combinatorial cassette mutagenesis as a probe of the information content of protein sequences. Science 241: 53-57.
20. Hermes, J.D., S.M. Parekh, S.C. Blacklow, H. Köster, and J.R. Knowles. 1989. A reliable method for random mutagenesis: the generation of mutant libraries using spiked oligodeoxynucleotide primers. Gene 84: 143-151.