A lock-docking oligo(dT) primer for 5′ and 3′ RACE PCR

Genome Research

Volumn 2, Issue 2, 1992, Pages 144-148

  Borson, Nancy D ^a   Salo, Wilmar L ^a   Drewes, Lester R ^a  

^a UNIVERSITY OF MINNESOTA DULUTH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; GLUCOSE TRANSPORTER; GLUCOSE TRANSPORTER 3; NERVE PROTEIN; POLYTHYMIDYLIC ACID;

ANIMAL; ARTICLE; DOG; GENETICS; METHODOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION;

ANIMALS; BASE SEQUENCE; DNA; DOGS; GLUCOSE TRANSPORTER TYPE 3; MOLECULAR SEQUENCE DATA; MONOSACCHARIDE TRANSPORT PROTEINS; NERVE TISSUE PROTEINS; POLY T; POLYMERASE CHAIN REACTION;

EID: 85045496218     PISSN: 10889051     EISSN: None     Source Type: Journal    
DOI: 10.1101/gr.2.2.144     Document Type: Article

References (24)

1. Frohman, M.A. 1990. RACE: Rapid amplification of cDNA ends. In PCR protocols: A guide to methods and applications (eds. M.A. Innis, D.H. Gelfand, J.J. Sninsky, and T.J. White), pp. 28-38. Academic, San Diego.
2. Frohman, M.A., M.K. Dush, and G.R. Martin. 1988. Rapid production of full-length cDNAs from rare transcripts: Amplification using a single gene-specific oligonucleotide primer. Proc. Natl. Acad. Sci. 85: 8998-9002.
3. Al-Ubaidi, M.R., S.J. Pittler, M.S. Champagne, J.T. Triantafyllos, J.F. McGinnis, and W. Baehr. 1990. Mouse opsin: gene structure and molecular basis of multiple transcripts. J. Biol. Chem. 265: 20563-20569.
4. Frohman, M.A. and G.R. Martin. 1989. Rapid amplification of cDNA ends using nested primers. Technique 1: 165-170.
5. Dumas, J.B., M. Edwards, J. Delort, and J. Mallet. 1991. Oligodeoxyribonucleotide ligation to single-stranded cDNAs: A new tool for cloning 5′ ends of mRNAs and for constructing cDNA libraries by in vitro amplification. Nucleic Acids Res. 19: 5227-5232.
6. Fritz, J.D., M.L Greaser, and J.A. Wolff. 1991. A novel 3′ extension technique using random primers in RNA-PCR. Nucleic Acids Res. 19: 3747.
7. Jain, R., R.H. Gomer, and J.J. Murtagh, Jr. 1992. Increasing specificity from the PCR-RACE technique. BioTechniques 12: 58-59.
8. Gerhart, D.Z., M.A. Broderius, N.D. Bor-son, and L.R. Drewes. 1992. Neurons and microvessels express the brain glucose transporter protein GLUT3. Proc. Natl. Acad. Sci. 89: 733-737.
9. Nagamatsu, S., J.M. Kornhauser, C.F. Burant, S. Seino, K.E. Mayo, and G.I. Bell. 1992. Glucose transporter expression in brain: cDNA sequence of mouse Glut3, the brain facilitative glucose transporter isoform, and identification of sites of expression by in situ hybridization. J. Biol. Chem. 267: 467-472.
10. Maher, F., S. Vannucci, J. Takeda, and I.A. Simpson. 1992. Expression of mouse-Glut3 and human-Gluts glucose transporter proteins in brain. Biochem. Biophys. Res. Commun. 182: 703-711.
11. Gerhart, D.Z., R.J. LeVasseur, M.A. Broderius, and L.R. Drewes. 1989. Glucose transporter localization in brain using light and electron immunocytochemistry. J. Neurosci. Res. 22: 464-472.
12. Pardridge, W.M., R.J. Boado, and C.R. Farrell. 1990. Brain-type glucose transporter (Glut-1) is selectively localized to the blood-brain barrier. J. Biol. Chem. 265: 18035-18040.
13. Kayano, T., H. Fukumoto, R.L. Eddy, Y. Fan, M.G. Byers, T.B. Shows, and G. I. Bell. 1988. Evidence for a family of human glucose transporter-like proteins: sequence and gene localization of a protein expressed in fetal skeletal muscle and other tissues. J. Biol. Chem. 263: 15245-15248.
14. Mueckler, M., C. Caruso, S.A. Baldwin, M. Panico, I. Blench, H.R. Morris, W.J. Allard, G.E. Lienhard, and H.F. Lodish. 1985. Sequence and structure of a human glucose transporter. Science 299: 941-945.
15. Birnbaum, M.J., H.C. Haspel, and O.M. Rosen. 1986. Cloning and characterization of a cDNA encoding the rat brain glucose-transporter protein. Proc. Natl. Acad. Sci. 83: 5784-5788.
16. Asano, T., Y. Shibasaki, M. Kasuga, Y. Kanazawa, F. Takaku, Y. Akanuma, and Y. Oka. 1988. Cloning of a rabbit brain glucose transporter cDNA and alteration of glucose transporter mRNA during tissue development. Biochem. Biophys. Res. Commun. 154: 1204-1211.
17. Weiler-Guttler, H., H. Zinke, B. Mockel, A. Frey, and H.G. Gassen. 1989. cDNA cloning and sequence analysis of the glucose transporter from porcine blood-brain barrier. Biol. Chem. Hoppe-Seyler 370: 467-473.
18. Kaestner, K. H., R.J. Christy, J.C. McLenithan, L.T. Braiterman, P. Cornelius, P.H. Pekala, and D.M. Lane. 1989. Sequence, tissue distribution, and differential expression of mRNA for a putative insulin-responsive glucose transporter in mouse 3T3-L1 adipocytes. Proc. Natl. Acad. Sci. 86: 3150-3154.
19. Thweatt, R., S. Goldstein, and R.J. Shmookler Reis. 1990. A universal primer mixture for sequence determination at the 3′ ends of cDNAs. Anal. Biochem. 190: 314-316.
20. Don, R.H., P.T. Cox, B.J. Wainwright, K. Baker, and J.S. Mattick. 1991. "Touch-down" PCR to circumvent spurious priming during gene amplification. Nucleic Acids Res. 19: 4008.
21. Schwarz, K., T. Hansen-Hagge, and C. Bartram. 1990. Improved yields of long PCR products using gene 32 protein. Nucleic Acids Res. 18: 1079.
22. Dr. Daniel Becker, personal communication.
23. Higuchi, R. 1989. Using PCR to engineer DNA. In PCR technology: Principles and applications for DNA amplification (ed. H.A. Erlich), pp. 61-70. Stockton Press, New York.
24. Paabo, S. and A.C. Wilson. 1988. Polymerase chain reaction reveals cloning artifacts. Nature 334: 387-388.