A one-step multiplex real-time RT-PCR for the universal detection of all currently known CCHFV genotypes

Journal of Virological Methods

Volumn 255, Issue , 2018, Pages 38-43

  Sas, Miriam A ^a   Vina Rodriguez, Ariel ^a   Mertens, Marc ^a   Eiden, Martin ^a   Emmerich, Petra ^b,c   Chaintoutis, Serafeim C ^d   Mirazimi, Ali ^e   Groschup, Martin H ^a  

^a FEDERAL RESEARCH INSTITUTE FOR ANIMAL HEALTH   (Germany)

^b BERNHARD NOCHT INSTITUTE FOR TROPICAL MEDICINE   (Germany)

^c UNIVERSITY OF ROSTOCK   (Germany)

^d ARISTOTLE UNIVERSITY OF THESSALONIKI   (Greece)

^e KAROLINSKA INSTITUTE   (Sweden)

Author keywords

CCHFV; Crimean Congo hemorrhagic fever virus; Multiplex RT qPCR

Indexed keywords

PRIMER DNA; VIRUS RNA;

ARTICLE; BOVINE; CAMEL; CONTROLLED STUDY; CRIMEAN CONGO HEMORRHAGIC FEVER; CRIMEAN-CONGO HEMORRHAGIC FEVER VIRUS; GENE SEQUENCE; GENOTYPE; HARE; HEDGEHOG; HUMAN; HYALOMMA; IXODIDAE; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SEROCONVERSION; SEROPREVALENCE; VIREMIA; VIRUS DETECTION; VIRUS STRAIN; VIRUS TRANSMISSION; ANIMAL; CLASSIFICATION; GENETICS; PROCEDURES; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; VIROLOGY;

ANIMALS; GENOTYPE; HEMORRHAGIC FEVER VIRUS, CRIMEAN-CONGO; HEMORRHAGIC FEVER, CRIMEAN; MULTIPLEX POLYMERASE CHAIN REACTION; REAL-TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; RNA, VIRAL; SENSITIVITY AND SPECIFICITY;

EID: 85042065393     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2018.01.013     Document Type: Article

References (22)

1. Atkinson, B., Chamberlain, J., Logue, C.H., Cook, N., Bruce, C., Dowall, S.D., Hewson, R., Development of a real-time RT-PCR assay for the detection of Crimean-Congo hemorrhagic fever virus. Vector Borne Zoonotic Dis. 12 (2012), 786–793.
2. Bente, D.A., Forrester, N.L., Watts, D.M., Mcauley, A.J., Whitehouse, C.A., Bray, M., Crimean-Congo hemorrhagic fever: history, epidemiology, pathogenesis, clinical syndrome and genetic diversity. Antivir. Res. 100 (2013), 159–189.
3. Carroll, S.A., Bird, B.H., Rollin, P.E., Nichol, S.T., Ancient common ancestry of Crimean-Congo hemorrhagic fever virus. Mol. Phylogenet. Evol. 55 (2010), 1103–1110.
4. Deyde, V.M., Khristova, M.L., Rollin, P.E., Ksiazek, T.G., Nichol, S.T., Crimean-Congo hemorrhagic fever virus genomics and global diversity. J. Virol. 80 (2006), 8834–8842.
5. Gonzalez, J.P., Camicas, J.L., Cornet, J.P., Faye, O., Wilson, M.L., Sexual and transovarian transmission of Crimean-Congo haemorrhagic fever virus in Hyalomma truncatum ticks. Res. Virol. 143 (1992), 23–28.
6. Hoffmann, B., Depner, K., Schirrmeier, H., Beer, M., A universal heterologous internal control system for duplex real-time RT-PCR assays used in a detection system for pestiviruses. J. Virol Methods 136 (2006), 200–209.
7. Holland, J., Esteban, D., Origin and evolution of viruses. Virus Genes 16:1 (1998), 13–21.
8. Hoogstraal, H., The epidemiology of tick-borne Crimean-Congo hemorrhagic fever in Asia, Europe, and Africa. J. Med. Entomol. 15 (1979), 307–417.
9. Jaaskelainen, A.J., Kallio-Kokko, H., Ozkul, A., Bodur, H., Korukruoglu, G., Mousavi, M., Pranav, P., Vaheri, A., Mirazimi, A., Vapalahti, O., Development and evaluation of a real-time RT-qPCR for detection of Crimean-Congo hemorrhagic fever virus representing different genotypes. Vector Borne Zoonotic Dis. 14 (2014), 870–872.
10. Logan, T.M., Linthicum, K.J., Bailey, C.L., Watts, D.M., Moulton, J.R., Experimental transmission of Crimean-Congo hemorrhagic fever virus by Hyalomma truncatum Koch. Am. J. Trop Med. Hyg. 40 (1989), 207–212.
11. Lukashev, A.N., Klimentov, A.S., Smirnova, S.E., Dzagurova, T.K., Drexler, J.F., Gmyl, A.P., Phylogeography of Crimean Congo hemorrhagic fever virus. PLoS One, 11, 2016, e0166744.
12. Mertens, M., Schmidt, K., Ozkul, A., Groschup, M.H., The impact of Crimean-Congo hemorrhagic fever virus on public health. Antivir. Res. 98 (2013), 248–260.
13. OIE, Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2016. (Online). http://www.oie.int/international-standard-setting/terrestrial-manual/access-online/. (Accessed 28th November 2016). 2016.
14. Sikorsky, J.A., Primerano, D.A., Fenger, T.W., Denvir, J., Effect of DNA damage on PCR amplification efficiency with the relative threshold cycle method. Biochem. Biophys Res. Commun. 323 (2004), 823–830.
15. Sikorsky, J.A., Primerano, D.A., Fenger, T.W., Denvir, J., DNA damage reduces Taq DNA polymerase fidelity and PCR amplification efficiency. Biochem. Biophys. Res. Commun. 355 (2007), 431–437.
16. Vina-Rodriguez, A., Eiden, M., Groschup, M.H., 2017. VisualOligoDeg: visual selection of PCR and microarray primers and probes from aligned sequences (in press).
17. Weaver, S.C., Evolutionary influences in arboviral disease. Curr. Top. Microbiol. Immunol. 299 (2006), 285–314.
18. Whitehouse, C.A., Crimean-Congo hemorrhagic fever. Antivir. Res. 64 (2004), 145–160.
19. Wolfel, R., Paweska, J.T., Petersen, N., Grobbelaar, A.A., Leman, P.A., Hewson, R., Georges-Courbot, M.C., Papa, A., Gunther, S., Drosten, C., Virus detection and monitoring of viral load in Crimean-Congo hemorrhagic fever virus patients. Emerg. Infect. Dis. 13 (2007), 1097–1100.
20. Yang, G.P., Erdman, D.D., Tondella, M.L., Fields, B.S., Evaluation of tetramethylrhodamine and black hole quencher 1 labeled probes and five commercial amplification mixes in TaqMan real-time RT-PCR assays for respiratory pathogens. J. Virol. Methods 162 (2009), 288–290.
21. Yen, Y.C., Kong, L.X., Lee, L., Zhang, Y.Q., LI, F., Cai, B.J., Gao, S.Y., Characteristics of Crimean-Congo hemorrhagic fever virus (Xinjiang strain) in China. Am. J. Trop. Med. Hyg. 34 (1985), 1179–1182.
22. Yilmaz, G.R., Buzgan, T., Irmak, H., Safran, A., Uzun, R., Cevik, M.A., Torunoglu, M.A., The epidemiology of Crimean-Congo hemorrhagic fever in Turkey, 2002–2007. Int. J. Infect. Dis. 13 (2009), 380–386.