Whole genome analysis of hypervirulent Klebsiella pneumoniae isolates from community and hospital acquired bloodstream infection

BMC Microbiology

Volumn 18, Issue 1, 2018, Pages

  Shankar, Chaitra ^a   Veeraraghavan, Balaji ^a   Nabarro, Laura Eve Buchnan ^a   Ravi, Raji ^a   Ragupathi, Naveen Kumar Devanga ^a   Rupali, Priscilla ^a  

^a CHRISTIAN MEDICAL COLLEGE   (India)

Author keywords

Bacteremia; Hypervirulent; K. pneumoniae; K. quasipneumoniae; Virulence factors; Whole genome sequencing

Indexed keywords

AEROBACTIN; ALLANTOIN; ENTEROCHELIN; SIDEROPHORE; YERSINIABACTIN; BACTERIAL PROTEIN; RMPA PROTEIN, KLEBSIELLA PNEUMONIAE; RMPA2 PROTEIN, KLEBSIELLA PNEUMONIAE; TRANSCRIPTION FACTOR; VIRULENCE FACTOR;

ADULT; AGED; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTEREMIA; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIAL VIRULENCE; BACTERIUM ISOLATE; BLOODSTREAM INFECTION; CHRONIC LIVER DISEASE; CLINICAL ARTICLE; CLINICAL OUTCOME; COMMUNITY ACQUIRED INFECTION; CONTROLLED STUDY; DISEASE ASSOCIATION; FEMALE; GENETIC CODE; GENETIC RESISTANCE; GENOTYPE; HOSPITAL INFECTION; HUMAN; IRON TRANSPORT; KLEBSIELLA PNEUMONIAE; KLEBSIELLA QUASIPNEUMONIAE; MALE; MOLECULAR EPIDEMIOLOGY; MULTIDRUG RESISTANCE; PHENOTYPE; POLYMERASE CHAIN REACTION; PREVALENCE; RMPA GENE; RMPA2 GENE; WHOLE GENOME SEQUENCING; BACTERIAL CAPSULE; BACTERIAL GENOME; CROSS INFECTION; GENETICS; INDIA; ISOLATION AND PURIFICATION; KLEBSIELLA INFECTION; LIVER DISEASE; MICROBIAL SENSITIVITY TEST; MICROBIOLOGY; MIDDLE AGED; PATHOGENICITY; PHYLOGENY; TREATMENT OUTCOME; VERY ELDERLY; VIRULENCE;

ADULT; AGED; AGED, 80 AND OVER; BACTEREMIA; BACTERIAL CAPSULES; BACTERIAL PROTEINS; COMMUNITY-ACQUIRED INFECTIONS; CROSS INFECTION; FEMALE; GENES, BACTERIAL; GENOME, BACTERIAL; GENOTYPE; HUMANS; INDIA; KLEBSIELLA INFECTIONS; KLEBSIELLA PNEUMONIAE; LIVER DISEASES; MALE; MICROBIAL SENSITIVITY TESTS; MIDDLE AGED; MOLECULAR EPIDEMIOLOGY; PHYLOGENY; SIDEROPHORES; TRANSCRIPTION FACTORS; TREATMENT OUTCOME; VIRULENCE; VIRULENCE FACTORS; WHOLE GENOME SEQUENCING;

EID: 85041955452     PISSN: None     EISSN: 14712180     Source Type: Journal    
DOI: 10.1186/s12866-017-1148-6     Document Type: Article

References (34)

1. Brisse S, Passet V, Grimont PA. Description of Klebsiella quasipneumoniae sp. nov., isolated from human infections, with two subspecies, Klebsiella quasipneumoniae subsp. quasipneumoniae subsp. nov. and Klebsiella quasipneumoniae subsp. similipneumoniae subsp. nov., and demonstration that Klebsiella singaporensis is a junior heterotypic synonym of Klebsiella variicola. Int J Syst Evol Microbiol. 2014;64(9):3146-52.
2. Shon AS, Bajwa RP, Russo TA. Hypervirulent (hypermucoviscous) Klebsiella pneumoniae: a new and dangerous breed. Virulence. 2013;4(2):107-18.
3. Breurec S, Melot B, Hoen B, Passet V, Schepers K, Bastian S, Brisse S. Liver abscess caused by infection with community-acquired Klebsiella quasipneumoniae subsp. quasipneumoniae. Emerg Infect Dis. 2016;22(3):529.
4. Wacharotayankun R, Arakawa Y, Ohta M, Tanaka K, Akashi T, Mori M, Kato N. Enhancement of extracapsular polysaccharide synthesis in Klebsiella pneumoniae by RmpA2, which shows homology to NtrC and FixJ. Infect Immun. 1993;61(8):3164-74.
5. Zhang Y, Zhao C, Wang Q, Wang X, Chen H, Li H, Zhang F, Li S, Wang R, Wang H. High prevalence of Hypervirulent Klebsiella pneumoniae infection in China: geographic distribution, clinical characteristics, and antimicrobial resistance. Antimicrob Agents Chemother. 2016;60(10):6115-20.
6. Compain F, Babosan A, Brisse S, Genel N, Audo J, Ailloud F, Kassis-Chikhani N, Arlet G, Decré D. Multiplex PCR for detection of seven virulence factors and K1/K2 capsular serotypes of Klebsiella pneumoniae. J Clin Microbiol. 2014;52(12):4377-80.
7. Versalovic J, Carroll KC, Funke G, Jorgensen JH, Landry ML, Warnock DW. Manual of clinical microbiology. 10th ed. Washington DC: American Society for Microbiology; 2010.
8. CLSI. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically. Approved Standard- Tenth Edition. CLSI document. M07- A10. Wayne: Clinical and Laboratory Standards Institute; 2015.
9. CLSI. Performance Standards for Antimicrobial Susceptibility Testing. Twenty fifth International Supplement. CLSI document M100-S25. Wayne: Clinical and Laboratory Standards Institute; 2015.
10. Brisse S, Fevre C, Passet V, Issenhuth-Jeanjean S, Tournebize R, Diancourt L, Grimont P. Virulent clones of Klebsiella Pneumoniae: identification and evolutionary scenario based on genomic and phenotypic characterization. PLoS One. 2009;4:3-e4982.
11. Turton JF, Baklan H, Siu LK, Kaufmann ME, Pitt TL. Evaluation of a multiplex PCR for detection of serotypes K1, K2 and K5 in Klebsiella sp. and comparison of isolates within these serotypes. FEMS Microbiol Lett. 2008;284(2):247-52.
12. Veeraraghavan B, Anandan S, Sekar SK, Gopi R, Ragupathi NK, Ramesh S, Verghese VP, Korulla S, Mathai S, Sangal L, Joshi S. First report on the draft genome sequences of Corynebacterium diphtheriae isolates from India. Genome Announce. 2016;4(6):e01316.
13. Shankar C, Nabarro LE, Ragupathi NK, Sethuvel DP, Daniel JL, Veeraraghavan B. Draft genome sequences of three hypervirulent carbapenem-resistant Klebsiella pneumoniae isolates from bacteremia. Genome Announce. 2016;4(6):e01081-16.
14. Shankar C, Santhanam S, Kumar M, Gupta V, Ragupathi NK, Veeraraghavan B. Draft genome sequence of an extended-Spectrum-β-Lactamase-positive Hypervirulent Klebsiella pneumoniae strain with novel sequence type 2318 isolated from a neonate. Genome Announce. 2016;4(6):e01273-16.
15. Shankar C, Nabarro LE, Sethuvel DP, Raj A, Ragupathi NK, Doss GP, Veeraraghavan B. Draft genome of a hypervirulent Klebsiella quasipneumoniae subsp. similipneumoniae with novel sequence type ST2320 isolated from a chronic liver disease patient. J Glob Antimicrob Resist. 2017;9:30-1.
16. SHV related to susceptibility to ampicillin in Klebsiella pneumoniae. Int J Antimicrob Agents. 2007;29(3):344-7.
17. Fang CT, Lai SY, Yi WC, Hsueh PR, Liu KL, Chang SC. Klebsiella pneumoniae genotype K1: an emerging pathogen that causes septic ocular or central nervous system complications from pyogenic liver abscess. Clin Infect Dis. 2007;45:284-93.
18. Lin YT, Jeng YY, Chen TL, Fung CP. Bacteremic community-acquired pneumonia due to Klebsiella pneumoniae: clinical and microbiological characteristics in Taiwan, 2001-2008. BMC Infect Dis. 2010;10(1):1.
19. Zhang Y, Zeng J, Liu W, Zhao F, Hu Z, Zhao C, Wang Q, Wang X, Chen H, Li H, Zhang F, Li S, Cao B, Wang H. Emergence of a hypervirulent carbapenem-resistant Klebsiella pneumoniae isolate from clinical infections in China. J Inf Secur. 2015;71(5):553-60.
20. Russo TA, Shon AS, Beanan JM, Olson R, MacDonald U, Pomakov AO, Visitacion MP. Hypervirulent K. pneumoniae secretes more and more active iron-acquisition molecules than "classical" K. pneumoniae thereby enhancing its virulence. PLoS One. 2011;6(10):e26734.
21. Jung SW, Chae HJ, Park YJ, Yu JK, Kim SY, Lee HK, Lee JH, Kahng JM, Lee SO, Lee MK, Lim JH. Microbiological and clinical characteristics of bacteraemia caused by the hypermucoviscosity phenotype of Klebsiella pneumoniae in Korea. Epidemiol Infect. 2013;141(02):334-40.
22. Sah JP, Shah SK, Sharma B, Shah M, Salahuddin M, Mahato RV, Yadav C, Razin MS, Rashid M. Siderophore production: a unique quality of pathogenic Klebsiella pneumonia to survive in low iron concentration. Asian J Med Biol Res. 2015;1(1):130-8.
23. Pomakova DK, Hsiao CB, Beanan JM, Olson R, MacDonald U, Keynan Y, Russo TA. Clinical and phenotypic differences between classic and hypervirulent Klebsiella pneumonia: an emerging and under-recognized pathogenic variant. Eur J Clin Microbiol Infect Dis. 2012;31(6):981-9.
24. Chen YT, Chang HY, Lai YC, Pan CC, Tsai SF, Peng HL. Sequencing and analysis of the large virulence plasmid pLVPK of Klebsiella pneumoniae CG43. Gene. 2004;337:189-98.
25. Tang HL, Chiang MK, Liou WJ, Chen YT, Peng HL, Chiou CS, Liu KS, Lu MC, Tung KC, Lai YC. Correlation between Klebsiella pneumoniae carrying pLVPK-derived loci and abscess formation. Eur J Clin Microbiol Infect Dis. 2010;29(6):689-98.
26. Hsieh PF, Lin TL, Lee CZ, Tsai SF, Wang JT. Serum-induced iron-acquisition systems and TonB contribute to virulence in Klebsiella pneumoniae causing primary pyogenic liver abscess. J Infect Dis. 2008;197:1717-27.
27. Russo TA, Olson R, MacDonald U, Beanan J, Davidson BA. Aerobactin, but not yersiniabactin, salmochelin, or enterobactin, enables the growth/survival of hypervirulent (hypermucoviscous) Klebsiella pneumoniae ex vivo and in vivo. Infect Immun. 2015;83(8):3325-33.
28. Lawlor MS, O'Connor C, Miller VL. Yersiniabactin is a virulence factor for Klebsiella pneumoniae during pulmonary infection. Infect Immun. 2007;75(3):1463-72.
29. Huang YJ, Liao HW, Wu CC, Peng HL. MrkF is a component of type 3 fimbriae in Klebsiella pneumoniae. Res Microbiol. 2009;160(1):71-9.
30. Lagos R, Baeza M, Corsini G, Hetz C, Strahsburger E, Castillo JA, Vergara C, Monasterio O. Structure, organization and characterization of the gene cluster involved in the production of microcin E492, a channel-forming bacteriocin. Mol Microbiol. 2001;42(1):229-43.
31. Yu WL, Ko WC, Cheng KC, Lee CC, Lai CC, Chuang YC. Comparison of prevalence of virulence factors for Klebsiella pneumoniae liver abscesses between isolates with capsular K1/K2 and non-K1/K2 serotypes. Diagn Microbiol Infect Dis. 2008;62(1):1-6.
32. Cubero M, Grau I, Tubau F, Pallarés R, Dominguez MA, Liñares J, Ardanuy C. Hypervirulent Klebsiella pneumoniae clones causing bacteraemia in adults in a teaching hospital in Barcelona, Spain (2007-2013). Clin Microbiol Infect. 2016;22(2):154-60.
33. Qu TT, Zhou JC, Jiang Y, Shi KR, Li B, Shen P, Wei ZQ, Yu YS. Clinical and microbiological characteristics of Klebsiella pneumoniae liver abscess in East China. BMC Infect Dis. 2015;15(1):1.
34. Luo Y, Wang Y, Ye L, Yang J. Molecular epidemiology and virulence factors of pyogenic liver abscess causing Klebsiella pneumoniae in China. Clin Microbiol Infect. 2014;20(11):O818-24.