Using total internal reflection fluorescence microscopy (Tirfm) to visualise insulin action

Neuromethods

Volumn 70, Issue , 2012, Pages 97-110

  Burchfield, James G ^a   Lopez, Jamie A ^a,b   Hughes, William E ^a,c  

^a GARVAN INSTITUTE OF MEDICAL RESEARCH   (Australia)

^b PETER MACCALLUM CANCER CENTRE   (Australia)

^c ST VINCENT S HOSPITAL   (Australia)

Author keywords

eGFP; Exocytosis; Fluorescence; GLUT4; Insulin; IRAP; Microscopy; pHluorin; TIRF; TIRFM; Trafficking

Indexed keywords

INSULIN;

3T3-L1 CELL LINE; ANIMAL CELL; CELL MIGRATION; ELECTROPORATION; EXOCYTOSIS; INSULIN LIKE ACTIVITY; INSULIN RESPONSE; NONHUMAN; PRIORITY JOURNAL; TOTAL INTERNAL REFLECTION FLUORESCENCE MICROSCOPY;

EID: 85037563497     PISSN: 08932336     EISSN: 19406045     Source Type: Book Series    
DOI: 10.1007/978-1-61779-897-9_5     Document Type: Chapter

References (7)

1. Burchfield JG, Lopez JA, Mele K, Vallotton P, Hughes WE (2010) Exocytotic vesicle behaviour assessed by total internal reflection fluorescence microscopy. Traffic 11:429–439
2. Lopez JA et al (2009) Identification of a distal GLUT4 trafficking event controlled by actin polymerization. Mol Biol Cell 20:3918–3929
3. Ng Y et al (2010) Cluster analysis of insulin action in adipocytes reveals a key role for Akt at the plasma membrane. J Biol Chem 285:2245–2257
4. Zhao P et al (2009) Variations in the requirement for v-SNAREs in GLUT4 trafficking in adipocytes. J Cell Sci 122:3472–3480
5. Rasband WS, Image J, U.S. National Institutes of Health. (Bethesda, Maryland, USA, http://imagej.nih.gov/ij/, 1997–2010)
6. Mele K, Burchfield JG, James DE, Vallotton P, Hughes WE (2009) Towards fully automated identification of vesicle-membrane fusion events in TIRF microscopy. Int J Comput Aided Eng Technol 1:502–515
7. Mele K et al (2009) Automatic identification of fusion events in TIRF microscopy image sequences. In: ICCV09 1st workshop on Video-oriented Object and Event Classification (VOEC’09), Kyoto, Japan, 2009