Single-stranded DNA library preparation does not preferentially enrich circulating tumor DNA

Clinical Chemistry

Volumn 63, Issue 10, 2017, Pages 1656-1659

  Moser, Tina ^a   Ulz, Peter ^a   Zhou, Qing ^a   Perakis, Samantha ^a   Geigl, Jochen B ^a   Speicher, Michael R ^a,b   Heitzer, Ellen ^a,b  

^a MEDICAL UNIVERSITY OF GRAZ   (Austria)

^b BIOTECHMED GRAZ   (Austria)

Author keywords

[No Author keywords available]

Indexed keywords

CHLOROPLAST DNA; DNA FRAGMENT; DOUBLE STRANDED DNA; SINGLE STRANDED DNA; CIRCULATING TUMOR DNA;

DNA LIBRARY; HUMAN; LETTER; NUCLEOSOME; PREGNANT WOMAN; BLOOD; CHEMICAL STRUCTURE; DNA SEQUENCE; GENE LIBRARY; GENETICS; PROCEDURES;

CIRCULATING TUMOR DNA; DNA, SINGLE-STRANDED; GENE LIBRARY; HUMANS; MOLECULAR STRUCTURE; SEQUENCE ANALYSIS, DNA;

EID: 85030773268     PISSN: 00099147     EISSN: 15308561     Source Type: Journal    
DOI: 10.1373/clinchem.2017.277988     Document Type: Letter

References (5)

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2. Underhill HR, Kitzman JO, Hellwig S, Welker NC, Daza R, Baker DN, et al. Fragment length of circulating tumor DNA. PLoS Genet 2016; 12: E1006162.
3. Burnham P, Kim MS, Agbor-Enoh S, Luikart H, Valantine HA, Khush KK, De Vlaminck I. Single-stranded DNA library preparation uncovers the origin and diversity of ultrashort cell-free DNA in plasma. Sci Rep 2016; 6: 27859.
4. Vong JSL, Tsang JCH, Jiang P, Lee WS, Leung TY, Chan KCA, et al. Single-stranded DNA library preparation preferentially enriches short maternal DNA in maternal plasma. Clin Chem 2017; 63: 1031-7.
5. Scheinin I, Sie D, Bengtsson H, van de Wiel MA, Olshen AB, van Thuijl HF, et al. DNA copy number analysis of fresh and formalin-fixed specimens by shallow whole-genome sequencing with identification and exclusion of problematic regions in the genome assembly. Genome Res 2014; 24: 2022-32.