A fatal outbreak of ST11 carbapenem-resistant hypervirulent Klebsiella pneumoniae in a Chinese hospital: a molecular epidemiological study

The Lancet Infectious Diseases

Volumn 18, Issue 1, 2018, Pages 37-46

  Gu, Danxia ^a,b   Dong, Ning ^c   Zheng, Zhiwei ^c   Lin, Di ^a   Huang, Man ^a   Wang, Lihua ^a   Chan, Edward Wai Chi ^c   Shu, Lingbin ^a   Yu, Jiang ^a   Zhang, Rong ^a   Chen, Sheng ^c  

^a THE SECOND AFFILIATED HOSPITAL OF ZHEJIANG UNIVERSITY SCHOOL OF MEDICINE   (China)

^b ZHEJIANG PROVINCIAL PEOPLE'S HOSPITAL   (China)

^c HONG KONG POLYTECHNIC UNIVERSITY   (Hong Kong)

Author keywords

[No Author keywords available]

Indexed keywords

AMIKACIN; AMOXICILLIN PLUS CLAVULANIC ACID; AZTREONAM; CARBAPENEM; CEFAZOLIN; CEFEPIME; CEFTRIAXONE; CIPROFLOXACIN; ERTAPENEM; GENTAMICIN; IMIPENEM; PIPERACILLIN PLUS TAZOBACTAM; TIGECYCLINE; TOBRAMYCIN;

ADULT; AGED; ANTIBIOTIC SENSITIVITY; ANTIBIOTIC THERAPY; ARTICLE; ARTIFICIAL VENTILATION; BACTERIAL STRAIN; BACTERIAL VIRULENCE; CHINA; CONTROLLED STUDY; DISEASE SEVERITY; GALLERIA MELLONELLA; HUMAN; INTENSIVE CARE UNIT; KLEBSIELLA PNEUMONIAE; LUNG INFECTION; MULTILOCUS SEQUENCE TYPING; MULTIPLE ORGAN FAILURE; MULTIPLE TRAUMA; PATIENT SELECTION; PRIORITY JOURNAL; SEPTIC SHOCK; TREATMENT RESPONSE; VENTILATOR ASSOCIATED PNEUMONIA; WHOLE GENOME SEQUENCING; ANIMAL; CARBAPENEM-RESISTANT ENTEROBACTERIACEAE; CLASSIFICATION; ENZYMOLOGY; EPIDEMIC; GENETICS; GENOTYPE; HOSPITAL; IMMUNOLOGY; ISOLATION AND PURIFICATION; KLEBSIELLA INFECTION; LEPIDOPTERA; MICROBIAL SENSITIVITY TEST; MICROBIOLOGY; MIDDLE AGED; MOLECULAR EPIDEMIOLOGY; NEUTROPHIL; PHYSIOLOGY; RETROSPECTIVE STUDY; SURVIVAL ANALYSIS; VIRULENCE;

AGED; ANIMALS; CARBAPENEM-RESISTANT ENTEROBACTERIACEAE; CHINA; DISEASE OUTBREAKS; GENOTYPE; HOSPITALS; HUMANS; KLEBSIELLA INFECTIONS; KLEBSIELLA PNEUMONIAE; LEPIDOPTERA; MICROBIAL SENSITIVITY TESTS; MIDDLE AGED; MOLECULAR EPIDEMIOLOGY; MULTILOCUS SEQUENCE TYPING; NEUTROPHILS; PNEUMONIA, VENTILATOR-ASSOCIATED; RETROSPECTIVE STUDIES; SURVIVAL ANALYSIS; VIRULENCE; WHOLE GENOME SEQUENCING;

EID: 85028557993     PISSN: 14733099     EISSN: 14744457     Source Type: Journal    
DOI: 10.1016/S1473-3099(17)30489-9     Document Type: Article

References (31)

1. Meatherall, BL, Gregson, D, Ross, T, Pitout, JD, Laupland, KB, Incidence, risk factors, and outcomes of Klebsiella pneumoniae bacteremia. Am J Med 122 (2009), 866–873.
2. Lin, YT, Liu, CJ, Chen, TJ, et al. Pyogenic liver abscess as the initial manifestation of underlying hepatocellular carcinoma. Am J Med 124 (2011), 1158–1164.
3. Shon, AS, Bajwa, RP, Russo, TA, Hypervirulent (hypermucoviscous) Klebsiella pneumoniae: a new and dangerous breed. Virulence 4 (2013), 107–118.
4. Alcantar-Curiel, MD, Giron, JA, Klebsiella pneumoniae and the pyogenic liver abscess: implications and association of the presence of rpmA genes and expression of hypermucoviscosity. Virulence 6 (2015), 407–409.
5. Chen, YT, Chang, HY, Lai, YC, Pan, CC, Tsai, SF, Peng, HL, Sequencing and analysis of the large virulence plasmid pLVPK of Klebsiella pneumoniae CG43. Gene 337 (2004), 189–198.
6. Tang, HL, Chiang, MK, Liou, WJ, et al. Correlation between Klebsiella pneumoniae carrying pLVPK-derived loci and abscess formation. Eur J Clin Microbiol Infect Dis 29 (2010), 689–698.
7. Yeh, KM, Kurup, A, Siu, LK, et al. Capsular serotype K1 or K2, rather than magA and rmpA, is a major virulence determinant for Klebsiella pneumoniae liver abscess in Singapore and Taiwan. J Clin Microbiol 45 (2007), 466–471.
8. Grundmann, H, Glasner, C, Albiger, B, et al. Occurrence of carbapenemase-producing Klebsiella pneumoniae and Escherichia coli in the European survey of carbapenemase-producing Enterobacteriaceae (EuSCAPE): a prospective, multinational study. Lancet Infect Dis 17 (2017), 153–163.
9. Zhang, R, Liu, L, Zhou, H, et al. Nationwide surveillance of clinical carbapenem-resistant enterobacteriaceae (CRE) strains in China. EBioMedicine 19 (2017), 98–106.
10. Chen, L, Mathema, B, Chavda, KD, DeLeo, FR, Bonomo, RA, Kreiswirth, BN, Carbapenemase-producing Klebsiella pneumoniae: molecular and genetic decoding. Trends Microbiol 22 (2014), 686–696.
11. Chen, L, Mathema, B, Pitout, JD, DeLeo, FR, Kreiswirth, BN, Epidemic Klebsiella pneumoniae ST258 is a hybrid strain. mBio 5 (2014), e01355–e01360.
12. CLSI. Performance standards for antimicrobial susceptibility testing; twenty-sixth informational supplement. CLSI document M100-S26, 2016, Clinical and Laboratory Standards Institute, Wayne, PA.
13. Turton, JF, Perry, C, Elgohari, S, Hampton, CV, PCR characterization and typing of Klebsiella pneumoniae using capsular type-specific, variable number tandem repeat and virulence gene targets. J Med Microbiol 59 (2010), 541–547.
14. Huang, Y, Yu, X, Xie, M, et al. Widespread dissemination of carbapenem-resistant escherichia coli sequence type 167 strains harboring blaNDM-5 in clinical settings in China. Antimicrob Agents Chemother 60 (2016), 4364–4368.
15. El-Mansi, M, Anderson, KJ, Inche, CA, Knowles, LK, Platt, DJ, Isolation and curing of the Klebsiella pneumoniae large indigenous plasmid using sodium dodecyl sulphate. Res Microbiol 151 (2000), 201–208.
16. Deleo, FR, Chen, L, Porcella, SF, et al. Molecular dissection of the evolution of carbapenem-resistant multilocus sequence type 258 Klebsiella pneumoniae. Proc Natl Acad Sci USA 111 (2014), 4988–4993.
17. Diago-Navarro, E, Chen, L, Passet, V, et al. Carbapenem-resistant Klebsiella pneumoniae exhibit variability in capsular polysaccharide and capsule associated virulence traits. J Infect Dis 210 (2014), 803–813.
18. McLaughlin, MM, Advincula, MR, Malczynski, M, Barajas, G, Qi, C, Scheetz, MH, Quantifying the clinical virulence of Klebsiella pneumoniae producing carbapenemase Klebsiella pneumoniae with a Galleria mellonella model and a pilot study to translate to patient outcomes. BMC Infect Dis, 14, 2014, 31.
19. Antipov, D, Korobeynikov, A, McLean, JS, Pevzner, PA, hybridSPAdes: an algorithm for hybrid assembly of short and long reads. Bioinformatics 32 (2016), 1009–1015.
20. Overbeek, R, Olson, R, Pusch, GD, et al. The SEED and the rapid annotation of microbial genomes using subsystems technology (RAST). Nucleic Acids Res 42:D1 (2014), D206–D214.
21. Seemann, T, Prokka: rapid prokaryotic genome annotation. Bioinformatics 30 (2014), 2068–2069.
22. Nabil-Fareed Alikhan, NKP, Zakour, NLB, Beatson, SA, BLAST ring image generator (BRIG): simple prokaryote genome comparisons. BMC Genomics, 12, 2011, 402.
23. Page, AJ, Cummins, CA, Hunt, M, et al. Roary: rapid large-scale prokaryote pan genome analysis. Bioinformatics 31 (2015), 3691–3693.
24. Wyres, KL, Wick, RR, Gorrie, C, et al. Identification of Klebsiella capsule synthesis loci from whole genome data. Microb Genom, 2, 2016, e000102.
25. Zankari, E, Hasman, H, Cosentino, S, et al. Identification of acquired antimicrobial resistance genes. J Antimicrob Chemother 67 (2012), 2640–2644.
26. Bialek-Davenet, S, Criscuolo, A, Ailloud, F, et al. Genomic definition of hypervirulent and multidrug-resistant Klebsiella pneumoniae clonal groups. Emerg Infect Dis 20 (2014), 1812–1820.
27. Sturn, A, Quackenbush, J, Trajanoski, Z, Genesis: cluster analysis of microarray data. Bioinformatics 18 (2002), 207–208.
28. Jiang, Y, Wei, Z, Wang, Y, Hua, X, Feng, Y, Yu, Y, Tracking a hospital outbreak of KPC-producing ST11 Klebsiella pneumoniae with whole genome sequencing. Clin Microbiol 21 (2015), 1001–1007.
29. Zhang, R, Lin, D, Chan, EW, Gu, D, Chen, GX, Chen, S, Emergence of carbapenem-resistant serotype K1 hypervirulent Klebsiella pneumoniae strains in China. Antimicrob Agents Chemother 60 (2015), 709–711.
30. Paczosa, MK, Mecsas, J, Klebsiella pneumoniae: going on the offense with a strong defense. Microbiol Mol Biol Rev 80 (2016), 629–661.
31. Aljanaby, AAJ, Alhasani, AHA, Virulence factors and antibiotic susceptibility patterns of multidrug resistance Klebsiella pneumoniae isolated from different clinical infections. African J Microbiol Res 10 (2016), 829–843.