A simple, flexible and high-throughput cloning system for plant genome editing via CRISPR-Cas system

Journal of integrative plant biology

Volumn 58, Issue 8, 2016, Pages 705-712

  Kim, Hyeran ^a   Kim, Sang Tae ^a   Ryu, Jahee ^a   Choi, Min Kyung ^a   Kweon, Jiyeon ^b   Kang, Beum Chang ^a   Ahn, Hyo Min ^a   Bae, Suji ^a   Kim, Jungeun ^a,b   Kim, Jin Soo ^a,b   Kim, Sang Gyu ^a  

^a Institute for Basic Science   (South Korea)

^b Seoul National University   (South Korea)

Author keywords

AarI mediated sgRNA cloning; CRISPR Cas9 T DNA binary vector; Exchangeable U6 U3 promoter; Gateway compatible Cas9 cloning

Indexed keywords

GUIDE RNA;

DNA; GENOME; PLANT; PROTEIN; RNA; TOBACCO;

ARABIDOPSIS; CRISPR CAS SYSTEM; GENE EDITING; GENE VECTOR; GENETIC TRANSFORMATION; GENETICS; MOLECULAR CLONING; NUCLEOTIDE SEQUENCE; PLANT GENE; PLANT GENOME; PROCEDURES; TRANSGENIC PLANT;

ARABIDOPSIS; BASE SEQUENCE; CLONING, MOLECULAR; CRISPR-CAS SYSTEMS; GENE EDITING; GENES, PLANT; GENETIC VECTORS; GENOME, PLANT; PLANTS, GENETICALLY MODIFIED; RNA, GUIDE; TRANSFORMATION, GENETIC;

AGROBACTERIUM; ANIMALIA; ARABIDOPSIS THALIANA; NICOTIANA ATTENUATA; NICOTIANA RUSTICA; STREPTOCOCCUS PYOGENES;

EID: 85027950432     PISSN: None     EISSN: 17447909     Source Type: Journal    
DOI: 10.1111/jipb.12474     Document Type: Article

References (45)