Basic techniques in mammalian cell tissue culture

Current Protocols in Toxicology

Volumn 2016, Issue , 2016, Pages A.3B.1-A.3B.22

  Phelan, Katy ^a   May, Kristin M ^b  

^a Florida Cancer Specialists   (United States)

^b Children's Hospital   (United States)

Author keywords

Aseptic technique; Freezing cells; Mammalian cells; Medium; Passaging cells; Tissue culture

Indexed keywords

ANIMAL; CELL CULTURE; CELL CULTURE TECHNIQUE; CRYOPRESERVATION; CULTURE MEDIUM; FREEZING; HUMAN; MAMMAL; PRIMARY CELL CULTURE; PROCEDURES; STANDARDS; TISSUE CULTURE TECHNIQUE; TISSUE PRESERVATION;

ANIMALS; CELL CULTURE TECHNIQUES; CELLS, CULTURED; CRYOPRESERVATION; CULTURE MEDIA; FREEZING; HUMANS; MAMMALS; PRIMARY CELL CULTURE; TISSUE CULTURE TECHNIQUES; TISSUE PRESERVATION;

EID: 85026650593     PISSN: 19349254     EISSN: 19349262     Source Type: Journal    
DOI: 10.1002/cptx.13     Document Type: Article

References (25)

1. Burgener, J. 2006. Position paper on the use of ultraviolet lights in biological safety cabinets. Appl. Biosaf. 11:228-230.
2. Coté, R. J. 1998. Aseptic technique for cell culture. Curr. Protoc. Cell Biol. 00:1.3.1-1.3.10. doi: 10.1002/0471143030.cb0103s00.
3. Coté, R.J. 1999a. Sterilization and filtration. Curr. Protoc. Cell Biol. 1:1.4.1-1.4.21. doi: 10.1002/0471143030.cb0104s01.
4. Coté, R. 1999b. Assessing and controlling microbial contamination in cell cultures. Curr. Protoc. Cell Biol. 1:1.5.1-1.5.18. doi: 10.1002/ 0471143030.cb0105s01.
5. Freshney, R.I. 2010. Culture of Animal Cells. A Manual of Basic Technique and Specialized Applications, 6th ed. Wiley-Blackwell, New York.
6. Meechan, P.J. and Wilson, C. 2006. Use of ultraviolet lights in biological safety cabinets: A contrarian view. Appl. Biosaf. 11:222-227.
7. Newman, C. 2003. Serum-free cell culture—the ethical, scientific and economic choice. The Biomedical Scientist Sept:941-942.
8. Priest, J.H. 1997. General cell culture principles and fibroblast culture. In The AGT Cytogenetics Laboratory Manual, 3rd ed. (M.J. Barch, T. Knutsen, and J.L. Spurbeck, eds.). pp. 173-197. Lippincott-Raven, Philadelphia.
9. Rooney, D.E. (ed). 2001. Human Cytogenetics: Constitutional Analysis: A Practical Approach, 3rd ed. Oxford University Press, New York.
10. Sato, J. D. and Kan, M. 1998. Media for culture of mammalian cells. Curr. Protoc. Cell Biol. 00:1.2.1-1.2.15. doi: 10.1002/ 0471143030.cb0102s00.
11. Triglia, R.P. and Linscott, W.D. 1980. Titers of nine complement components, conglutinin and C3b inactivator in adult and fetal bovine sera. Mol. Immunol. 17:741-748.
12. Uphoff, C.C. and Drexler, H.G. 2014. Eradication of mycoplasma contaminations from cell cultures. Curr. Protoc. Mol. Biol. 106:28.5:28.5.1-28.5.12. doi: 10.1002/0471142727.mb2805s106.
13. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS Publication No. (CDC) 21-1112. Washington DC, U.S. Governmental Printing Office:2009. Available at http://cdc.gov/biosafety/publications/bmbl5/BMBL.pdf.
14. Volokhov, D.V., Graham, L.J., Brorson, K.A., and Chizhikov, V.E. 2011. Mycoplasma testing of cell substrates and biologics: Review of alternative non-microbiological techniques. Mol. Cell. Probes 25:69-77.
15. Freshney, R.I. 2010. Culture of Animal Cells. A Manual of Basic Technique and Special Applications, 6th ed. Wiley-Blackwell, New York
16. http://www.atcc.org
17. The Web site of the American Type Culture Collection, a nonprofit biological resource center, has an excellent document library for various cell culture topics.
18. http://www.coriell.org
19. The Web site for the Coriell Institute for Medical Research
20. http://www.lifetechnologies.com/us/en/home/ technical-resources/technical-reference-library.html
21. This Web site has documents about general cell culture, protocols, and troubleshooting.
22. http://www.lifescience.roche.com
23. The document resources section of the Roche Life Science site contains a technique guide for culture of animal cells.
24. http://www.phe-culturecollections.org.uk
25. The Web site for Public Health England includes four separate collections of cell lines and microbial strains. A free handbook on cell culture is available in the section for the European Collection of Cell Cultures (ECACC).