Rapid identification of a Mycobacterium tuberculosis full genetic drug resistance profile through whole genome sequencing directly from sputum

International Journal of Infectious Diseases

Volumn 62, Issue , 2017, Pages 44-46

  Nimmo, Camus ^a   Doyle, Ronan ^a   Burgess, Carrie ^a   Williams, Rachel ^a   Gorton, Rebecca ^a   McHugh, Timothy D ^a   Brown, Mike ^b   Morris Jones, Stephen ^b   Booth, Helen ^b   Breuer, Judith ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

^b UNIVERSITY COLLEGE LONDON HOSPITALS   (United Kingdom)

Author keywords

Clinical samples; Directly from sputum; Drug resistance; Tuberculosis; Whole genome sequencing

Indexed keywords

AMIKACIN; CYCLOSERINE; ETHAMBUTOL; ETHIONAMIDE; ISONIAZID; LINEZOLID; MOXIFLOXACIN; PYRAZINAMIDE; QUINOLONE DERIVATIVE; RIFAMPICIN; TUBERCULOSTATIC AGENT;

ACID FAST BACTERIUM; ADULT; ARTICLE; BACTERIAL MUTATION; BACTERIUM IDENTIFICATION; CASE REPORT; COUGHING; DISEASE DURATION; DRUG MEGADOSE; DRUG WITHDRAWAL; FEMALE; GENE MUTATION; GENETIC ANALYZER; GENETIC RESISTANCE; HUMAN; MICROSCOPY; MULTIDRUG RESISTANT TUBERCULOSIS; MYCOBACTERIUM TUBERCULOSIS; NIGERIAN; NIGHT SWEAT; SPUTUM ANALYSIS; TREATMENT DURATION; TREATMENT OUTCOME; WHOLE GENOME SEQUENCING; ANTIBIOTIC RESISTANCE; DRUG EFFECTS; GENETICS; ISOLATION AND PURIFICATION; LUNG TUBERCULOSIS; MICROBIOLOGY; MUTATION; SPUTUM;

ADULT; ANTITUBERCULAR AGENTS; DRUG RESISTANCE, BACTERIAL; FEMALE; HUMANS; ISONIAZID; MUTATION; MYCOBACTERIUM TUBERCULOSIS; RIFAMPIN; SPUTUM; TUBERCULOSIS, PULMONARY; WHOLE GENOME SEQUENCING;

EID: 85026353944     PISSN: 12019712     EISSN: 18783511     Source Type: Journal    
DOI: 10.1016/j.ijid.2017.07.007     Document Type: Article

References (5)

1. Brown, A.C., Bryant, J.M., Einer-Jensen, K., Holdstock, J., Houniet, D.T., Chan, J.Z., et al. Rapid Whole-Genome Sequencing of Mycobacterium tuberculosis Isolates Directly from Clinical Samples. J Clin Microbiol 53:7 (2015), 2230–2237.
2. Eilertson, B., Maruri, F., Blackman, A., Herrera, M., Samuels, D.C., Sterling, T.R., High proportion of heteroresistance in gyrA and gyrB in fluoroquinolone-resistant Mycobacterium tuberculosis clinical isolates. Antimicrob Agents Chemother 58:6 (2014), 3270–3275.
3. Machado, D., Perdigão, J., Ramos, J., Couto, I., Portugal, I., Ritter, C., et al. High-level resistance to isoniazid and ethionamide in multidrug-resistant Mycobacterium tuberculosis of the Lisboa family is associated with inhA double mutations. J Antimicrob Chemother 68:8 (2013), 1728–1732.
4. Pankhurst, L.J., Del Ojo Elias, C., Votintseva, A.A., Walker, T.M., Cole, K., Davies, J., et al. Rapid, comprehensive, and affordable mycobacterial diagnosis with whole-genome sequencing: a prospective study. Lancet Respir Med 4:1 (2016), 49–58.
5. Votintseva, A.A., Bradley, P., Pankhurst, L., Del Ojo Elias, C., Loose, M., Nilgiriwala, K., et al. Same-day diagnostic and surveillance data for tuberculosis via whole genome sequencing of direct respiratory samples. J Clin Microbiol, 2017.