Rapid multiplex creation of Escherichia coli strains capable of interfering with phage infection through CRISPR

Methods in Molecular Biology

Volumn 1311, Issue , 2015, Pages 147-159

  Strotksaya, Alexandra ^a   Semenova, Ekaterina ^a   Savitskaya, Ekaterina ^a   Severinov, Konstantin ^a  

^a NONE

Author keywords

Bacteriophage; Cas proteins; CRISPR; Escherichia coli; Spacers; Strain construction

Indexed keywords

BACTERIAL DNA; BACTERIOPHAGE DNA; CELLULAR APOPTOSIS SUSCEPTIBILITY PROTEIN; GENOMIC DNA; OLIGONUCLEOTIDE; PLASMID DNA; SPACER DNA;

ARTICLE; BACTERIAL CELL; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIOPHAGE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CONTROLLED STUDY; CRISPR CAS SYSTEM; ENTEROBACTERIA PHAGE M13; ENTEROBACTERIA PHAGE T4; ESCHERICHIA COLI; GENE CASSETTE; NONHUMAN; PENICILLIN RESISTANCE; PRIORITY JOURNAL; PROMOTER REGION; BACTERIUM TRANSFORMATION; GENETIC ENGINEERING; GENETICS; IMMUNOLOGY; PHYSIOLOGY; PLASMID; PROCEDURES; VIROLOGY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

BACTERIOPHAGES; CRISPR-CAS SYSTEMS; ESCHERICHIA COLI; GENETIC ENGINEERING; PLASMIDS; TRANSFORMATION, BACTERIAL;

EID: 85025147704     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-2687-9_9     Document Type: Article

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