Preparation of cell cultures and vaccinia virus stocks

Current Protocols in Molecular Biology

Volumn 2017, Issue , 2017, Pages

  Cotter, Catherine A ^a   Earl, Patricia L ^a   Wyatt, Linda S ^a   Moss, Bernard ^a  

^a NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

Author keywords

Cell culture; Modified vaccinia virus Ankara; Vaccinia virus

Indexed keywords

VIRUS DNA;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL LINE; CHEMICAL PROCEDURES; CHICK EMBRYO; EMBRYO; FIBROBLAST; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; NONHUMAN; PRIORITY JOURNAL; TITRIMETRY; TRYPSINIZATION; VACCINIA VIRUS; VIRUS PURIFICATION; VIRUS STRAIN; ANIMAL; CELL CULTURE TECHNIQUE; GROWTH, DEVELOPMENT AND AGING; ISOLATION AND PURIFICATION; PROCEDURES; VIROLOGY;

ANIMALS; CELL CULTURE TECHNIQUES; CELL LINE; CELLS, CULTURED; CHICK EMBRYO; DNA, VIRAL; FIBROBLASTS; HUMANS; VACCINIA VIRUS;

EID: 85024841426     PISSN: 19343639     EISSN: 19343647     Source Type: Journal    
DOI: 10.1002/cpmb.33     Document Type: Article

References (10)

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2. Carroll, M.W. and Moss, B. 1997. Host range and cytopathogenicity of the highly attenuatedMVA strain of vaccinia virus: Propagation and generation of recombinant viruses in a nonhuman mammalian cell line. Virology 238:198-211. doi: 10.1006/viro.1997.8845.
3. Elroy-Stein, O. and Moss, B. 2001. Gene expression using the vaccinia virus/T7 RNA polymerase hybrid system. Curr. Protoc. Mol. Biol. 43:16.19.1-16.19.11. doi: 10.1002/0471142727.mb1619s43.
4. Linscott, W.D. 1998. Linscott's Directory of Immunological and Biological Reagents, 10th ed. W.D. Linscott, Santa Rosa, CA.
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6. Meyer, H., Sutter, G., and Mayer, A. 1991. Mapping of deletions in the genome of the highly attenuated vaccinia virus MVA and their influence on virulence. J. Gen. Virol. 72:1031-1038. doi: 10.1099/0022-1317-72-5-1031.
7. Moss, B. 2013. Poxviridae. In Fields Virology, Sixth ed., Vol. 2 (D.M. Knipe and P.M. Howley, eds.) pp. 2129-2159.Wolters Kluwer/Lippincott Williams & Wilkins, Philadelphia.
8. Phelan, M.C. 2006. Techniques for mammalian cell tissue culture. Curr. Protoc. Mol. Biol. 74:A.3F.1-A.3F.18. doi: 10.1002/0471142727.mba03fs74.
9. Sutter, G. and Moss, B. 1992. Nonreplicating vaccinia virus efficiently expresses recombinant genes. Proc. Natl. Acad. Sci. U.S.A. 89:10847-10851. doi: 10.1073/pnas.89.22.10847.
10. Wyatt, L.S., Earl, P.L., and Moss, B. 2015. Generation of recombinant vaccinia viruses. Curr. Protoc. Micribiol. 117:16.17.1-16.17.18. doi: 10.1002/cpmb.32.