Large-scale benchmarking reveals false discoveries and count transformation sensitivity in 16S rRNA gene amplicon data analysis methods used in microbiome studies

Microbiome

Volumn 4, Issue 1, 2016, Pages 62-

  Thorsen, Jonathan ^a   Brejnrod, Asker ^a   Mortensen, Martin ^a   Rasmussen, Morten A ^a   Stokholm, Jakob ^a   Al Soud, Waleed Abu ^a   Sørensen, Søren ^a   Bisgaard, Hans ^a   Waage, Johannes ^a  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

16S sequencing; Benchmark; Beta diversity; Differential relative abundance; Microbiome

Indexed keywords

RNA 16S;

BACTERIUM; BENCHMARKING; BIOLOGY; CASE CONTROL STUDY; CLASSIFICATION; FALSE POSITIVE RESULT; GENETICS; HIGH THROUGHPUT SEQUENCING; HUMAN; MICROFLORA; NUCLEOTIDE SEQUENCE; PROCEDURES; SEQUENCE ANALYSIS;

BACTERIA; BASE SEQUENCE; BENCHMARKING; CASE-CONTROL STUDIES; COMPUTATIONAL BIOLOGY; FALSE POSITIVE REACTIONS; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; HUMANS; MICROBIOTA; RNA, RIBOSOMAL, 16S; SEQUENCE ANALYSIS, RNA;

EID: 85020054792     PISSN: None     EISSN: 20492618     Source Type: Journal    
DOI: 10.1186/s40168-016-0208-8     Document Type: Article

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